A Preliminary Study of Callus for Inducing Cold Tolerance in Alfalfa Breeding

doi:10.11733/j.issn.1007-0435.2003.01.013

›› 2003, Vol. 11 ›› Issue (1): 63-66.DOI: 10.11733/j.issn.1007-0435.2003.01.013

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A Preliminary Study of Callus for Inducing Cold Tolerance in Alfalfa Breeding

LI Bo¹, LI Hong², WANG Xiu-li¹, YU Fei¹

1. Life Science and Engineering College, Qiqihar University, Qiqihar, Heilongjiang Province, 161006, China;
2. Heilongjiang Institute of Animal Science, Qiqihar, Heilongjiang Province, 161006, China

Received:2002-08-22 Revised:2002-12-03 Online:2003-02-15 Published:2003-02-15

苜蓿抗性愈伤组织诱导的初步研究

李波¹, 李红², 王秀丽¹, 于非¹

1. 齐齐哈尔大学生命科学与工程学院, 黑龙江, 齐齐哈尔, 161006;
2. 黑龙江省畜牧研究所, 黑龙江, 齐齐哈尔, 161000

作者简介:李波(1962- ),女,辽宁省鞍山市人,副教授,从事牧草抗性研究,植物组织培养和细胞生物学教学与研究
基金资助:
黑龙江省教育委员会科研基金资助项目(2002)

Abstract

Abstract: MS was used as a fundamental medium for alfalfa breeding.DES and UV were successively used for callus mutation and selection.Eventually,some callus showed certain degree of cold tolerance.

Key words: Alfalfa, Callus, DES, UV

摘要： 以硫酸二乙酯(DES)和紫外线(UV)对准格尔苜蓿愈伤组织进行诱变处理,筛选其半致死浓度和剂量。对诱变处理后的材料进行脯氨酸含量测定。结果表明,脯氨酸含量明显高于对照。筛选出具有抗性的苜蓿愈伤组织,为苜蓿抗性育种提供基础材料。

关键词: 苜蓿, 愈伤组织, 硫酸二乙酯, 紫外线

CLC Number:

Q813.1

LI Bo, LI Hong, WANG Xiu-li, YU Fei. A Preliminary Study of Callus for Inducing Cold Tolerance in Alfalfa Breeding[J]. , 2003, 11(1): 63-66.

李波, 李红, 王秀丽, 于非. 苜蓿抗性愈伤组织诱导的初步研究[J]. , 2003, 11(1): 63-66.

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A Preliminary Study of Callus for Inducing Cold Tolerance in Alfalfa Breeding

苜蓿抗性愈伤组织诱导的初步研究

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