Detecition of Genetic Diversity of Stipa grandis in Inner Mongolia Using RAPD Markers

doi:10.11733/j.issn.1007-0435.2003.02.014

›› 2003, Vol. 11 ›› Issue (2): 170-177,188.DOI: 10.11733/j.issn.1007-0435.2003.02.014

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Detecition of Genetic Diversity of Stipa grandis in Inner Mongolia Using RAPD Markers

ZHANG Hong-mei¹, ZHAO Meng-li², LI Qing-feng², HAN Bing³, SUO Pei-feng³

1. Jiaxing Academy of Agricultural Sciences, Jiaxing Zhejiang Province 314016, China;
2. College of Ecology and Environmental Science, Inner Mongolia Agricultural University, Huhhot Inner Mongolia Autonomous Region 010019, China;
3. Department of Biology, Inner Mongolia Agricultural University, Huhhot Inner Mongolia Autonomous Region 010018, China

Received:2002-08-29 Revised:2002-11-19 Online:2003-05-15 Published:2003-05-15

内蒙古地区大针茅群体遗传多样性RAPD研究

张红梅¹, 赵萌莉², 李青丰², 韩冰³, 索培芬³

1. 浙江省嘉兴市农业科学院, 嘉兴, 314016;
2. 内蒙古农业大学生态环境学院, 呼和浩特, 010019;
3. 内蒙古农业大学生物工程学院, 呼和浩特, 010018

作者简介:张红梅,26岁,女,助理农艺师,主要从事分子生态学研究
基金资助:
国家自然基金(30060015);内蒙古自然基金(20001303)资助

Abstract

Abstract: Random amplified polymorphic DNA(RAPD) analyses were used to estimate the genetic diversity of different populations of Stipa grandis and their distribution, as well as the ecological factors in relation to the genetic differentiation of S.grandis. Among a total of 134 bands perceived via 16 primers from 7 populations of S.grandis in different regions,120 bands or 89.55% are polymorphic, showing the high genetic variation of individuals. Most of the molecular variation(58.66%) of the 7 S.grandis populations exists within each of the populations, while only 41.34% exists among the populations. The specific loci of the 7 populations of S.grandis are closely related to their geographical distribution.

Key words: Stipa grandis, RAPD, Genetic diversity, Ecological differentiation

摘要： 应用RAPD技术检测大针茅植物7个群体的遗传多样性及其分布,探索生态因子与遗传分化的相互联系。在大针茅7个群体中,利用16个有效引物共获得134条谱带,其中多态性谱带120条,占89.55%,说明个体间发生较高的遗传变异。7个大针茅群体遗传变异大部分存在于群体内(58.66%),只有少部分存在于群体之间(41.34%)。7个群体的特异性位点与其地理分布范围相关。

关键词: 大针茅, RAPD, 遗传多样性, 生态分化

CLC Number:

S812

ZHANG Hong-mei, ZHAO Meng-li, LI Qing-feng, HAN Bing, SUO Pei-feng. Detecition of Genetic Diversity of Stipa grandis in Inner Mongolia Using RAPD Markers[J]. , 2003, 11(2): 170-177,188.

张红梅, 赵萌莉, 李青丰, 韩冰, 索培芬. 内蒙古地区大针茅群体遗传多样性RAPD研究[J]. , 2003, 11(2): 170-177,188.

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Detecition of Genetic Diversity of Stipa grandis in Inner Mongolia Using RAPD Markers

内蒙古地区大针茅群体遗传多样性RAPD研究

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