Co-transformation of bar and P5CS Gene Using Bombardment on Lolium perenne L.

doi:10.11733/j.issn.1007-0435.2005.01.009

›› 2005, Vol. 13 ›› Issue (1): 34-38.DOI: 10.11733/j.issn.1007-0435.2005.01.009

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Co-transformation of bar and P5CS Gene Using Bombardment on Lolium perenne L.

YANG Cheng-min^1,2, WANG Hong-zhi¹, SUN Zhen-yuan³, WEI Jian-hua¹

1. Beijing Institute of Agriculture and Forestry, Beijing 100089 China;
2. College of Agronomy, Inner Mongolia Agriculture University, Hohhot, Inner Mongolia Autonomous Region 010018 China;
3. Laboratory of Biotechnology, CAF, Beijing 100091 China

Received:2003-11-20 Revised:2004-04-26 Online:2005-02-15 Published:2005-02-15

利用基因枪共转化法获得转bar与P5CS基因黑麦草

杨成民^1,2, 王宏芝¹, 孙振元³, 魏建华¹

1. 北京生物技术研究中心, 北京, 100089;
2. 内蒙古农业大学农学院, 呼和浩特, 010018;
3. 中国林业科学研究院生物技术实验室, 北京, 100083

通讯作者: 魏建华
作者简介:杨成民(1978- ),男,内蒙古通辽市人,内蒙古农业大学硕士生,研究方向为植物基因工程
基金资助:
国家转基因专项(J-2002-B-006)

Abstract

Abstract: The conditions of inducing the high qualified calli of perennial ryegrass mature embryo were studied. The genes of bar and P5CS were transformed into the calli using microprojectile bombardment. Under the selection of 1.2‰ Glufosinate resistance, some transformed plants were obtained. PCR analysis indicated that 18 transgenic plants contained bar gene and the frequency of transformation was 0.72%. Five of these 18 transgenic regenerate plants contained P5CS gene and the co-transformation frequency was 27.8%.

Key words: Perennial ryegrass, Mature embryo, bar gene, P5CS gene

摘要： 以多年生黑麦草(Lolium perenne L.)种子成熟胚为外植体进行高效诱导愈伤组织的组培条件,在此基础上用基因枪法轰击其成熟胚的愈伤组织,共转化分别携带bar基因和P5CS基因表达框架的2种质粒,经1.2‰草丁磷(Glufosinate)抗性筛选获得了转基因再生植株.经聚合酶链式反应(PCR)检测筛选到整合bar基因的株系18个,遗传转化率为0.72%,其中5个检测到P5CS,共转化效率为27.8%.

关键词: 黑麦草, 成熟胚, bar基因, P5CS基因

CLC Number:

Q943.1

YANG Cheng-min, WANG Hong-zhi, SUN Zhen-yuan, WEI Jian-hua. Co-transformation of bar and P5CS Gene Using Bombardment on Lolium perenne L.[J]. , 2005, 13(1): 34-38.

杨成民, 王宏芝, 孙振元, 魏建华. 利用基因枪共转化法获得转bar与P5CS基因黑麦草[J]. , 2005, 13(1): 34-38.

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Co-transformation of bar and P5CS Gene Using Bombardment on Lolium perenne L.

利用基因枪共转化法获得转bar与P5CS基因黑麦草

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