Effects of Adding Molasses and Lactic Acid Bacteria on the Fermentation Quality of Mixed Silage of Oat Straw and Perennial Ryegrass

doi:10.11733/j.issn.1007-0435.2014.02.031

›› 2014, Vol. 22 ›› Issue (2): 409-413.DOI: 10.11733/j.issn.1007-0435.2014.02.031

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Effects of Adding Molasses and Lactic Acid Bacteria on the Fermentation Quality of Mixed Silage of Oat Straw and Perennial Ryegrass

GUO Gang¹, YUAN Xian-jun¹, LIN Yuan-yuan¹, WEN Ai-you², RONG Hui¹, SHEN Zhen-xi³, YU Cheng-qun³, SHAO Tao¹

1. Institute of Ensiling and Processing of Grass, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China;
2. College of Animal Science, Anhui Science and Technology University, Fengyang, Anhui Province 233100, China;
3. Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 1001013, China

Received:2013-08-05 Revised:2013-10-23 Online:2014-04-15 Published:2014-04-21

添加糖蜜与乳酸菌对燕麦秸杆和黑麦草混合青贮品质的影响

郭刚¹, 原现军¹, 林园园¹, 闻爱友², 荣辉¹, 沈振西³, 余成群³, 邵涛¹

1. 南京农业大学动物科学技术学院饲草调制加工与贮藏研究所, 江苏南京 210095;
2. 安徽科学技术学院动物科学技术学院, 安徽凤阳 233100;
3. 中国科学院地理科学与资源研究所, 北京 100101

通讯作者: 邵涛
作者简介:郭刚（1984-），男，山西太原人，博士研究生，主要从事粗饲料加工与利用研究，E-mail：guosteel1984@126.com
基金资助:
“十二五”国家科技支撑计划“藏北退化草地综合整治技术与示范”（2010BAE00739-03-03-02）；“十二五”西藏重大科技专项“优质青贮饲料生产、加工和贮藏技术研究与示范”；中国科学院西藏区域创新平台建设项目“促进农牧民增收的西藏农牧结合技术体系构建与示范”资助

Abstract

Abstract: To improve the fermentation quality of mixed silage of oat straw and perennial ryegrass and enhance the utilization efficiency of oat straw resource in Tibet, the mixture of oat straw and perennial ryegrass (4:6) were ensiled with different additives. The treatments were designed as follows: control, lactic acid bacteria (LAB, 1×10⁶ cfu·g^-1FW), molasses (4% FW) and LAB + molasses, six reduplicate silos per treatment. The fermentation quality was analyzed after ensiling 60 days. Molasses or/and LAB additives significantly decreased the pH value, ammonia/total N (AN/TN), propionic and butyric acid contents of mixed silage (P<0.05), while significantly increased the lactic/acetic acid ratio and lactic acid contents of mixed silage compared to control silage (P<0.05). The silage added with molasses had higher lactic acid and water soluble carbohydrate (WSC) contents than the one added with LAB. However, there was no significantly different fermentation quality between the silage added with molasses and the one added with LAB + molasses. Therefore, it was suggested that 4% molasses additive was suitable to improve the fermentation quality of mixed silage of oat straw and perennial ryegrass in Tibet.

Key words: Oat straw, Perennial ryegrass, Additives, Mixed ensilage, Fermentation quality

摘要： 为探讨西藏地区燕麦（Avena sativa L.）秸秆和多年生黑麦草（Lolium perenne L.）混合青贮使用添加剂改善发酵品质的措施，进一步提高燕麦秸秆利用效率，将收获籽粒后的燕麦秸秆和抽穗期刈割的多年生黑麦草以4∶6混合青贮，分别添加1×10⁶ cfu·g^-1 FW的乳酸菌制剂、4% FW糖蜜和乳酸菌+糖蜜，青贮60 d后取样分析青贮饲料发酵品质。结果表明：单独或组合添加糖蜜和乳酸菌均不同程度的改善了混合青贮发酵品质，显著降低了pH值、氨态氮/总氮、丙酸和丁酸含量（P<0.05），显著提高了乳酸含量和乳酸/乙酸（P<0.05）。添加糖蜜组发酵品质优于添加乳酸菌组，显示较高的乳酸和水溶性碳水化合物含量（P<0.05），且单独添加糖蜜与组合添加组各发酵指标无显著差异。综合考虑，在燕麦秸秆和多年生黑麦草（4∶6）混合青贮时添加4%糖蜜较为适宜。

关键词: 燕麦秸秆, 多年生黑麦草, 添加剂, 混合青贮, 发酵品质

CLC Number:

S816.53

GUO Gang, YUAN Xian-jun, LIN Yuan-yuan, WEN Ai-you, RONG Hui, SHEN Zhen-xi, YU Cheng-qun, SHAO Tao. Effects of Adding Molasses and Lactic Acid Bacteria on the Fermentation Quality of Mixed Silage of Oat Straw and Perennial Ryegrass[J]. , 2014, 22(2): 409-413.

郭刚, 原现军, 林园园, 闻爱友, 荣辉, 沈振西, 余成群, 邵涛. 添加糖蜜与乳酸菌对燕麦秸杆和黑麦草混合青贮品质的影响[J]. , 2014, 22(2): 409-413.

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References

[1] 孙小龙,周禾,李平,等. 苜蓿与玉米秸秆混贮研究[J]. 草业学报,2009,18(5):86-92
[2] 王昆昆,玉柱,邵涛,等. 乳酸菌制剂对不同比例苜蓿和披碱草混贮发酵品质的影响[J]. 草业学报,2010,19(4):94-100
[3] 原现军,余成群,李志华,等. 西藏青稞秸秆与多年生黑麦草混合青贮发酵品质的研究[J]. 草业学报,2012,21(4):325-330
[4] 林园园. 西藏农作物秸秆与苇状羊茅和多年生黑麦草混合青贮发酵品质的研究[D]. 南京：南京农业大学,2012
[5] Lima R, Lourenco M, Diaz R F, et al. Effect of combined ensiling of sorghum and soybean with or without molasses and lactobacilli on silage quality and in vitro rumen fermentation [J]. Animal Feed Science and Technology,2010,155(2/4): 122-131
[6] Weinberg Z G, Chen Y, Weinberg P. Ensiling olive cake with and without molasses for ruminant feeding [J]. Bioresource Technology,2008,99(6):1526-1529
[7] Filya I, Muck RE, Contreras-Govea FE. Inoculant effects on alfalfa silage: Fermentation products and nutritive value [J]. Journal Of Dairy Science,2007,90(11):5108-5114
[8] Hashemzadeh-Cigari F, Khorvash M, Ghorbani G R, et al. Interactive effects of molasses by homofermentative and heterofermentative inoculants on fermentation quality, nitrogen fractionation, nutritive value and aerobic stability of wilted alfalfa (Medicago sativa L) silage [J]. Journal of Animal Physiology and Animal Nutrition,2013,doi: 10.1111/jpn.12079
[9] Shao T, Zhang L, Shimojo M, et al. Fermentation quality of Italian ryegrass (Lolium multiflorum Lam.) silages treated with encapsulated-glucose, glucose, sorbic acid and pre-fermented juices [J]. Asian-Australasian Journal of Animal Sciences,2007,20(11):1699-1704
[10] Weinberg Z G, Shatz O, Chen Y, et al. Effect of lactic acid bacteria inoculants on in vitro digestibility of wheat and corn silages [J]. Journal of Dairy Science,2007,90(10):4754-4762
[11] Madrid J, Martínez-Teruel A, Hernández F, et al. A comparative study on the determination of lactic acid in silage juice by colorimetric, high-performance liquid chromatography and enzymatic methods [J]. Journal of the Science of Food and Agriculture,1999,79(12):1722-1726
[12] Weatherburn M. Phenol-hypochlorite reaction for determination of ammonia [J]. Analytical Chemistry,1967,39(8):971-974
[13] Shao T, Zhang Z X, Shimojo M, et al. Comparison of fermentation characteristics of italian ryegrass (Lolium multiflorum Lam.) and guineagrass (Panicum maximum Jacq.) during the early stage of ensiling [J]. Asian-Australasian Journal of Animal Sciences,2005,18(12):1727-1734
[14] 自给饲料品质评价研究会. 粗饲料品质评价手册[M]. 东京:日本草地畜产种子协会,2001:82-87
[15] Bolsen K K. Silage fermentation and silage additives: Review [J]. Asian-Australasian Journal of Animal Sciences,1996,9(5):483-493
[16] McDonald P, Henderson A R, Heron S J E. The biochemistry of silage [M]. 2th ed. Aberystwyth: Cambrian Printers Ltd 1991
[17] Zhang J G, Kawamoto H, Cai Y M. Relationships between the addition rates of cellulase or glucose and silage fermentation at different temperatures [J]. Animal Science Journal,2010,81(3):325-330
[18] 原现军,夏坤,余成群,等. 添加青稞酒糟对西藏地区箭筈豌豆与苇状羊茅青贮发酵品质的影响[J]. 畜牧兽医学报,2012,43(9):1408-1414
[19] Wilkinson J M, Davies D R. The aerobic stability of silage: Key findings and recent developments [J]. Grass and Forage Science,2013,68(1):1-19
[20] Merry R J, Davies D R. Propionibacteria and their role in the biological control of aerobic spoilage in silage [J]. Le Lait, 1999,79(1):149-164

Effects of Adding Molasses and Lactic Acid Bacteria on the Fermentation Quality of Mixed Silage of Oat Straw and Perennial Ryegrass

添加糖蜜与乳酸菌对燕麦秸杆和黑麦草混合青贮品质的影响

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