Effect of Adding Moringa Oleifera Leaves and Lactobacillus farciminis on Quality of Corn Stover Silage

doi:10.11733/j.issn.1007-0435.2021.06.026

Acta Agrestia Sinica ›› 2021, Vol. 29 ›› Issue (6): 1343-1349.DOI: 10.11733/j.issn.1007-0435.2021.06.026

Previous Articles Next Articles

Effect of Adding Moringa Oleifera Leaves and Lactobacillus farciminis on Quality of Corn Stover Silage

LYU Hong-jian¹, ZOU Xuan², WU Shuo², LI Yun³, CHEN Xiao-yang², ZHANG Qing²

1. College of Animal Science, South China Agricultural University, Guangzhou, Guangdong Province 510642, China;
2. College of Forestry and Landscape Architecture, South China Agricultural University, Guangdong Research Center of Woody Forage Engineering Technology Guangzhou, Guangdong Key Laboratory for Innovation Development and Utilization of Forage Plant Germplasm, Guangzhou, Guangdong Province 510642, China;
3. College of Biological Science and Technology, Beijing Forestry University, Beijing 100083, China

Received:2020-12-14 Revised:2021-01-08 Online:2021-06-15 Published:2021-07-05

添加辣木叶和香肠乳杆菌对玉米秸秆青贮品质的影响

吕竑建¹, 邹璇², 吴硕², 李云³, 陈晓阳², 张庆²

1. 华南农业大学动物科学学院, 广东广州 510642;
2. 华南农业大学林学与风景园林学院, 广东木本饲料工程技术研究中心, 广东省森林植物种质创新与利用重点实验室, 广东广州 510642;
3. 北京林业大学生物科学与技术学院, 北京 100083

通讯作者: 张庆,E-mail:zqing1988@126.com;陈晓阳,E-mail:xychen@scau.edu.cn
作者简介:吕竑建(1994-)，男，广东肇庆人，硕士研究生，主要从事于动物营养与饲料科学的研究，E-mail：379941622@qq.com
基金资助:
广东省林业科技创新项目（2018KJCX001，2019KJCX001）；广东省自然基金项目（2020A1515011253）共同资助

Abstract

Abstract: To investigate the effect of adding Moringa oleifera leaves (M) and lactic acid bacteria on the quality and aerobic stability of corn stover silage. Lactobacillus farciminis (LF) was select and identified from M.oleifera leaves silage. Ensiling different proportion of M mixed with corn stover with or without LF,a two-factor (M×LF) factorial design was completely randomized and after 60 days the silages were opened to determine and analysis the quality and aerobic stability, the 6 treatments were CK,20%M,40%M,CK+LF,20%M+LF and 40%M+LF. The results showed that adding M along could significantly decrease pH,the contents of NH₄⁺-N,acetic acid,NDF and ADF,while increased the contents of crude protein and lactic acid. Adding M could decreased aerobic stability of corn stover silage,but the combination with LF could improve it. M contain high proportion of proteins,and could improved the nutrition and quality of corn stover silage. Ensiling with LF could improve the aerobic stability of corn stover silage mixed with M.

Key words: Moringa oleifera, Corn stover, Lactic acid bacteria, Silage fermentation quality, Aerobic stability

摘要： 本研究选用从辣木叶（Moringa Oleifera，M）青贮中分离并鉴定出的香肠乳杆菌（Lactobacillus farciminis，LF），将其加入到辣木叶与玉米（Zea mays）秸秆青贮中，探究添加辣木叶和香肠乳杆菌对玉米秸秆青贮品质及其有氧稳定性的影响。本试验采用双因素（M×LF）完全随机设计，共6个试验组，分别为CK，20%M，40%M，CK+LF，20%M+LF和40%M+LF，在青贮第60 d时开袋取样进行相关指标测定。结果表明，添加辣木叶可显著降低pH值、降低氨态氮、乙酸、中性洗涤纤维和酸性洗涤纤维的含量（P<0.05）；显著增加粗蛋白质和乳酸含量（P<0.05）；添加LF可以减少酵母菌和大肠杆菌的数量（P<0.05）；添加辣木叶使玉米秸秆青贮的有氧稳定性下降，但添加LF可明显改善有氧稳定性。辣木叶粗蛋白质含量丰富，与玉米秸秆混合青贮可提升营养价值，改善青贮品质，配合LF青贮能得到更好的有氧稳定性。

关键词: 辣木, 玉米秸秆, 乳酸菌, 青贮发酵品质, 有氧稳定性

CLC Number:

S816.5+3

LYU Hong-jian, ZOU Xuan, WU Shuo, LI Yun, CHEN Xiao-yang, ZHANG Qing. Effect of Adding Moringa Oleifera Leaves and Lactobacillus farciminis on Quality of Corn Stover Silage[J]. Acta Agrestia Sinica, 2021, 29(6): 1343-1349.

吕竑建, 邹璇, 吴硕, 李云, 陈晓阳, 张庆. 添加辣木叶和香肠乳杆菌对玉米秸秆青贮品质的影响[J]. 草地学报, 2021, 29(6): 1343-1349.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2021.06.026

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2021/V29/I6/1343

References

[1] 联合国粮食及农业组织[EB/OL].http://www.fao.org/faostat/en/#data/QC,2019-10-12/2020-12-14
[2] Sun H,Cui X,Stinner W,et al. Synergetic effect of combined ensiling of freshly harvested and excessively wilted maize stover for efficient biogas production[J]. Bioresource Technology,2019,285:121338
[3] Zhao Y,Damgaard A,Xu Y,et al. Bioethanol from corn stover -Global warming footprint of alternative biotechnologies[J]. Applied Energy,2019:237-253
[4] He L,LV H,Wang C,et al. Dynamics of fermentation quality,physiochemical property and enzymatic hydrolysis of high-moisture corn stover ensiled with sulfuric acid or sodium hydroxide[J]. Bioresource Technology,2020,298:122510
[5] Ni K,Zhao J,Zhu B,et al. Assessing the fermentation quality and microbial community of the mixed silage of forage soybean with crop corn or sorghum[J]. Bioresource Technology,2018:563-567
[6] Gopalakrishnan L,Doriya K,Kumar D S,et al. Moringa oleifera:A review on nutritive importance and its medicinal application[J]. Food Science and Human Wellness,2016,5(2):49-56
[7] Rockwood J L,Anderson B G,Casamatta D A. Potential uses of Moringa oleifera and an examination of antibiotic efficacy conferred by M. oleifera seed and leaf extracts using crude extraction techniques available to underserved indigenous populations[J]. International Journal of Phytotherapy Research,2013,3(2):61-71
[8] Aregheore E M. Intake and digestibility of Moringa oleifera-batiki grass mixtures by growing goats[J]. Small Ruminant Research,2002,46(1):23-28
[9] Richter,N,Perumal S,Becker,K. Evaluation of nutritional quality of moringa (Lam.) leaves as an alternative protein source for Nile tilapia (L.)[J]. Aquaculture,2003,217(1):599-611
[10] Rubanza C D K,Shem M N,Otsyina R,et al. Polyphenolics and tannins effect on in vitro digestibility of selected Acacia species leaves[J]. Animal Feed Science & Technology,2005,119(1-2):129-142
[11] Kholif A E,Gouda G A,Olafadehan O A,et al. Effects of replacement of Moringa oleifera for berseem clover in the diets of Nubian goats on feed utilisation,and milk yield,composition and fatty acid profile[J]. Animal,2018,12,(5):964-972
[12] Wang C,He L,Xing Y,et al. Fermentation quality and microbial community of alfalfa and stylo silage mixed with Moringa oleifera leaves[J]. Bioresource Technology,2019:240-247
[13] Wilkinson J M,Davies D R. The aerobic stability of silage:key findings and recent developments[J]. Grass and Forage Science,2013,68(1):1-19
[14] 李龙兴,龚正发,黎俊,等. 糖蜜和乳酸菌对去穗玉米秸秆青贮发酵品质的影响[J]. 草地学报.2018,26(4):1026-1029
[15] Ranjit N K,Kung L. The effect of Lactobacillus buchneri,Lactobacillus plantarum,or a chemical preservative on the fermentation and aerobic stability of corn silage[J]. Journal of Dairy Science,2000,83(3):526-535
[16] Wang Y,He L,Xing Y,et al. Bacterial diversity and fermentation quality of Moringa oleifera leaves silage prepared with lactic acid bacteria inoculants and stored at different temperatures[J]. Bioresource Technology.2019,284:349-358
[17] Zhang Q,Yu Z,Na R,et al. Effects of different additives on fermentation quality and aerobic stability of Leymus chinensis silage[J]. Grass and Forage Science,2018,73(2):413-419
[18] Broderick G A,Kang J H. Automated Simultaneous Determination of Ammonia and Total Amino Acids in Ruminal Fluid and In Vitro Media 1[J]. journal of dairy science,1980,63(1):64-75
[19] 美国分析化学家协会(AOAC),AOAC 981·10官方分析方法(第17版)[S].盖瑟斯堡:美国分析化学家协会出版社,2000:1339
[20] Licitra G,Hernandez T M,Van Soest P J,et al. Standardization of procedures for nitrogen fractionation of ruminant feeds[J]. Animal Feed Science and Technology,1996,57(4):347-358
[21] Van Soest P J,Robertson J B,Lewis B A,et al. Methods for Dietary Fiber,Neutral Detergent Fiber,and Nonstarch Polysaccharides in Relation to Animal Nutrition[J]. Journal of Dairy Science,1991,74(10):3583-3597
[22] 周瑞,刘立山,郎侠,等. 甜菜块根和玉米秸秆混贮营养品质及有氧暴露期微生物数量分析[J]. 草地学报.2019,27(1):192-198
[23] 王成,王益,周玮,等. 植物乳杆菌和含水量对辣木叶青贮品质和单宁含量的影响[J]. 草业学报,2019,28(6):109-118
[24] 张庆. 饲草青贮用乳酸菌的筛选及作用机理[D].北京:中国农业大学,2016:8
[25] Cai Y,Benno Y,Ogawa M,et al. Influence of Lactobacillus spp. from an inoculant and of Weissella and Leuconostoc spp. from forage crops on silage fermentation[J]. Applied and Environmental Microbiology,1998,64(8):2982-2987
[26] Guyader J,Baron V S,Beauchemin K A,et al. Corn Forage Yield and Quality for Silage in Short Growing Season Areas of the Canadian Prairies[J]. Agronomy,2018,8(9):164
[27] Ni K,Wang F,Zhu B,et al. Effects of lactic acid bacteria and molasses additives on the microbial community and fermentation quality of soybean silage[J]. Bioresource Technology,2017:706-715
[28] Muck R E. Silage microbiology and its control through additives[J]. Revista Brasileira De Zootecnia,2010:183-191
[29] Chen M,Verdes R P. Elucidation of bactericidal effects incurred by Moringa oleifera and chitosan[J]. Journal of the US,2009,4:65-79
[30] Ratshilivha N,Awouafack M D,Toit E S,et al. The variation in antimicrobial and antioxidant activities of acetone leaf extracts of 12Moringa oleifera (Moringaceae) trees enables the selection of trees with additional uses[J]. South African Journal of Botany,2014,92(92):59-64
[31] Ohmomo S,Tanaka O,Kitamoto H, et al. Silage and Microbial Performance,Old Story but New Problems[J]. Jarq-japan Agricultural Research Quarterly,2002,36(2):59-71
[32] Guo X S,Ding W R,Han J G,et al. Characterization of protein fractions and amino acids in ensiled alfalfa treated with different chemical additives[J]. Animal Feed Science and Technology,2008,142(1):89-98
[33] Mateos G G,Jimenezmoreno E,Serrano M P,et al. Poultry response to high levels of dietary fiber sources varying in physical and chemical characteristics[J]. The Journal of Applied Poultry Research,2012,21(1):156-174
[34] Gerlach K,Ros F,Weis K,et al. Changes in maize silage fermentation products during aerobic deterioration and effects on dry matter intake by goats[J]. Agricultural and Food Science,2013,22(1):168-181
[35] He L,Zhou W,Xing Y,et al. Improving the quality of rice straw silage with Moringa oleifera leaves and propionic acid:Fermentation,nutrition,aerobic stability and microbial communities[J]. Bioresource Technology,2020,299(3):122579

Effect of Adding Moringa Oleifera Leaves and Lactobacillus farciminis on Quality of Corn Stover Silage

添加辣木叶和香肠乳杆菌对玉米秸秆青贮品质的影响

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	CHEN Lei, BAO Xue-yan, GUO Gang, HUO Wen-jie, LI Qing-hong, XU Qing-fang, WANG Cong, LIU Qiang. Effects of Tannic Acid and Lactic Acid Bacteria on Alfalfa Silage Quality and Ruminal Fermentation in Vitro [J]. Acta Agrestia Sinica, 2021, 29(8): 1853-1858.
[2]	ZHENG Ming-yang, WU Shuo, GUO Xiang, CHEN Xiao-yang, ZHANG Qing. Effects of Lactobacillus and Cellulase on the Silage Quality of Amomum Villosum leaves [J]. Acta Agrestia Sinica, 2021, 29(5): 1113-1117.
[3]	WANG Li-xue, HAN Jing, CHEN Long-bin, YU Xin-yue, LIU Jing-xi, MA Yi, HUO Wen-juan. Effects of Various Lactic Acid Bacteria Combinations on Bacterial Community Structure of Alfalfa Silage [J]. Acta Agrestia Sinica, 2021, 29(2): 388-395.
[4]	ZHU Jiu-gang, ZHANG Jian, SHAO Tao, DONG Zhi-hao, LI Jun-feng, TANG Lu, RAN Qi-fan, LIU Qin-hua. The Effects of Additives on Fermentation Quality and Aerobic Stability of Whole-plant Oat Silage [J]. Acta Agrestia Sinica, 2020, 28(6): 1756-1761.
[5]	LIANG Long-fei, WANG Fei, DONG Xiang, HAO Jun. Fermentation Quality and Aerobic Stability of the Total Mixed Ration after Replacing Alfalfa with Different Rations of King Grass ‘Reyan No.4’ [J]. Acta Agrestia Sinica, 2020, 28(3): 703-711.
[6]	CHEN Die, LIN Kai-cheng, LIN Qin, CHEN Xia, CHEN Xinzhu. Isolation and Identification of Salt-Tolerant Lactic Acid Bacteria in Spartina anglica Hubb. and Its Effect on the Quality of Silage [J]. Acta Agrestia Sinica, 2020, 28(2): 565-570.
[7]	LI Long-xing, WANG Yi-ni, YANG Xue-dong, LI Jun, GONG Zheng-fa, FRASER George Harrison, CHEN Xiu-hua, HUANG Wen-hu, LIANG Xian-yi. Effects of Wilting and Adding Lactobacillus on Fermentation Quality of Coix lacryma-jobi Silage [J]. Acta Agrestia Sinica, 2019, 27(4): 1102-1106.
[8]	MU Lin, LI Shun, ZENG Ning-bo, CHEN Gui-hua, ZHANG Zhi-fei, YE Zhi-gang. Effect of Adding Molasses or Lactic Acid Bacteria on Quality of Mixed Silage of Amaranth and Rice Straws [J]. Acta Agrestia Sinica, 2019, 27(2): 482-487.
[9]	ZHOU Rui, LIU Liu-shan, LANG Xia, WANG Cai-lian, SONG Shu-zhen, WU Jian-ping. Analysis of Silage Quality and Microbe Quantity During Aerobic Exposure of Beet Root and Corn Stover Mixed-ensilage [J]. Acta Agrestia Sinica, 2019, 27(1): 192-198.
[10]	CHEN Xin-zhu, GAO Cheng-fang, LIU Jing, CHEN Bing-dian, HUANG Qin-lou, HUANG Xiu-sheng, WENG Bo-qi. Microbial Diversity of Fermented Juice Using Epiphytic Lactic Acid Bacteria and Their Effects of on the Fermentation Quality of Fungus Chaff Silage [J]. Acta Agrestia Sinica, 2019, 27(1): 199-210.
[11]	YUAN Shi-gai, CHEN Chao, ZHANG Ming-jun, YANG Feng, SHEN Lu-lu, ZHANG Tao-tao, HAO Jun. Effects of Additives on the Fermentation Quality and Aerobic Stability of Silage Corn under Warm and Wet Climate [J]. Acta Agrestia Sinica, 2018, 26(4): 942-947.
[12]	LI Long-xing, GONG Zheng-fa, LI Jun, ZHANG Ming-jing, WANG Shun-ying, ZHU Xin, WEI Fang-hong, LUO Yu-jie. The Effects of Molasses and Lactic Acid Bacteria on the Fermentation Quality of Ear-removed Corn Straws Silage [J]. Acta Agrestia Sinica, 2018, 26(4): 1026-1029.
[13]	ZHENG Yi, TANG Hong-yu, LI Tian-yu, CAI Mao, CHEN Hao, ZHANG Jia-bao, WANG Peng. Design and Verification of Measurement Equipment for Analysis of Silage Aerobic Stability [J]. Acta Agrestia Sinica, 2018, 26(1): 238-243.
[14]	SI Hua-zhe, LIU Han-lu, NAN Wei-xiao, JIN Chun-ai, LI Guang-yu. Effects of Different Lactic Acid Bacteria on Fermentation Characteristics and Aerobic Stability of Low Moisture Rice Stalk Silage [J]. Acta Agrestia Sinica, 2017, 25(6): 1294-1299.
[15]	JIANG Yi-bao, WANG Yang, LIU Zhen-yang, SUN Juan-juan, YAN Xue-bing, YU Zhu. Effect of Lactic Acid Bacteria on Silage Quality and the Content of Flavonoids of Alfalfa [J]. Acta Agrestia Sinica, 2017, 25(4): 896-900.