Acta Agrestia Sinica

Acta Agrestia Sinica ›› 2021, Vol. 29 ›› Issue (11): 2600-2608.DOI: 10.11733/j.issn.1007-0435.2021.11.027

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Effects of Cellulase and Xylanase on Fermentation Quality and in Vitro Digestibility Coefficient of Napier Grass

DING Hao1, WU Yong-jie1, SHAO Tao1, ZHAO Jie1, DAI Tong-tong1, DONG Dong1, YIN Xue-jing1, JIA Yu-shan2,3, LI Jun-feng1   

  1. 1. Institute of Ensiling and Processing of Grass, College of Agro-grassland Science, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China;
    2. Key Laboratory of Forage Cultivation, Processing and High Efficient Utilization, Ministry of Agriculture, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China;
    3. Key Laboratory of Grassland Resources, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China
  • Received:2021-08-19 Revised:2021-09-17 Online:2021-11-15 Published:2021-12-06

纤维素酶和木聚糖酶对象草青贮发酵品质及体外消化率的影响

丁浩1, 吴永杰1, 邵涛1, 赵杰1, 代童童1, 董东1, 尹雪敬1, 贾玉山2,3, 李君风1   

  1. 1. 南京农业大学草业学院饲草调制加工与贮藏研究所, 江苏 南京 210095;
    2. 内蒙古农业大学农业农村部饲草栽培加工与高效利用重点实验室, 内蒙古 呼和浩特 010018;
    3. 内蒙古农业大学教育部草地资源重点实验室, 内蒙古 呼和浩特 010018
  • 通讯作者: 李君风,E-mail:ljf126ff@126.com
  • 作者简介:丁浩(1998-),男,汉族,四川德阳人,硕士研究生,主要从事粗饲料加工与利用研究,E-mail:609461808@qq.com
  • 基金资助:
    国家自然科学基金区域创新发展联合基金重点支持项目（U20A2003）资助；国家自然科学基金青年项目（NO.31901391）资助

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Abstract: To explore the effects of cellulase and xylanase on the fermentation quality and in vitro digestibility coefficient of napier grass (Pennisetum purpureum Schumach) silage,the napier grass was ensiled with no additive control (CK),cellulase (CE),xylanase (XE),combined addition (CX cellulase+xylanase),respectively. The silos were opened after 7,30,60 and 90 days of ensiling to analyse fermentation quality. The results showed that CE,XE or CX addition significantly increased lactic acid content (P<0.05),reduced pH,ammonia nitrogen/total nitrogen and dry matter loss. The contents of neutral detergent fiber,acid detergent fiber,cellulose and hemicellulose of enzyme addition treatments were lower than those of CK,while the contents of water-soluble carbohydrates,glucose,fructose and sucrose were higher than CK. In the in vitro fermentation test,the dry matter digestibility,neutral detergent fiber digestibility,acid detergent digestibility and in vitro gas production of CE,XE and CX silages were significantly higher than CK (P<0.05). The CX had the highest in vitro gas production (P<0.05),and there was no significant difference between CE and XE. In summary,the addition of cellulase and xylanase can improve the fermentation quality and in vitro digestibility of napier grass silage,and the combined addition effect is better.

Key words: Napier grass, Cellulase, Xylanase, Fermentation quality, In vitro digestibility coefficient

摘要： 为探讨纤维素酶和木聚糖酶对象草（Pennisetum purpureum Schumach）青贮发酵品质与体外消化率的影响，试验设对照组（CK）、添加纤维素酶组（CE）、添加木聚糖酶组（XE）和组合添加酶组（CX，纤维素酶+木聚糖酶）。青贮7，30，60，90 d后开窖取样，测定相关指标。结果表明：与对照组相比，CE，XE和CX组显著提高了乳酸含量（P<0.05），降低了pH、氨态氮/总氮和干物质损失；添加酶处理组中性洗涤纤维（Neutral detergent fiber，NDF）、酸性洗涤纤维（Acid detergent fiber，ADF）、纤维素、半纤维素含量均低于对照，并且水溶性碳水化合物、葡萄糖、果糖和蔗糖含量增加；体外发酵试验中，CE，XE和CX组青贮饲料干物质消化率、中性洗涤纤维消化率、酸性洗涤消化率和体外产气均显著高于CK（P<0.05），且CX组体外产气量最高，而CE与XE之间差异不显著。综上，添加纤维素酶、木聚糖酶提高了象草青贮发酵品质与体外消化率，组合添加酶效果更好。

关键词: 象草, 纤维素酶, 木聚糖酶, 发酵品质, 体外消化率

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