Abstract: Effects of hormones and their combinations on callus induction,growth state,and shoot regeneration were studied using the mature embryos of Bothriochloa ischaemum(L.) Keng as example plants.At same time,in order to provide groundwork for the biotechnology breeding,the plant regeneration system of B.ischaemum was set up.Results indicate that the callus induction frequency reached 86.7% in the callus induction medium supplemented with 2.0 mg/L 2,4-D and similar effectiveness was detected between MSB and MS medium.Rapid growth of callus and embryogenesis were maintained in the callus subculture medium with addition of 1.0 mg/L 2,4-D.After callus subculture,the shoot regeneration rate from callus were 61.3% in the differentiation medium supplemented with 0.1 mg/L 6-BA and 0.05 mg/L NAA.and the root development was independent upon any hormone compositions.

Key words: Bothriochloa ischaemum(L.) Keng, Mature embryo, Embryogenic callus, Plant regeneration

摘要： 以白羊草(Bothriochloa ischaemum(L.)Keng)成熟胚为外植体,研究不同激素及其不同配比对愈伤组织诱导发生与生长状态及其绿苗分化能力的影响。从而建立了白羊草植株再生体系,为开展生物技术育种提供参考。结果表明:在愈伤组织诱导培养基中添加2.0 mg/L 2,4-D诱导频率为86.7%,MSB和MS培养基对愈伤组织诱导效果相近;在其继代培养基中添加1.0 mg/L 2,4-D愈伤组织增殖快,且保持胚胎发生能力;适宜的分化培养基为6-BA 0.1 mg/L+NAA 0.05 mg/L,分化率达61.3%;愈伤组织在不添加任何生长调节剂的培养基上即可生根。

关键词: 白羊草, 成熟胚, 愈伤组织, 植株再生