Estimation of Diet Composition and Herbage Intake of Grazing Cows using n-Alkane Technique in Inner Mongolia Typical Steppes

doi:10.11733/j.issn.1007-0435.2009.06.001

›› 2009, Vol. 17 ›› Issue (6): 693-698.DOI: 10.11733/j.issn.1007-0435.2009.06.001

Estimation of Diet Composition and Herbage Intake of Grazing Cows using n-Alkane Technique in Inner Mongolia Typical Steppes

LIU Gui-he^1,3, WANG Shi-ping², ZHANG Ying-jun¹, HAN Jian-guo¹

1. Institute of Grassland Science, China Agricultural University, Beijing 100193, China;
2. Key Laboratory of Evolution and Adaptation of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai Province 810008, China; 3.Department of Animal Husbandry and Engineering, Hebei North University, Zhangjiakou, Hebei Province 075131, China

Received:2009-06-02 Revised:2009-10-28 Online:2009-12-15 Published:2009-12-15

链烷技术估测典型草原放牧牛食性食量方法研究

刘贵河^1,3, 汪诗平², 张英俊¹, 韩建国¹

1. 中国农业大学草地研究所, 北京, 100193;
2. 河北北方学院牧业工程系, 河北, 张家口, 075000;;
3. 中国科学院西北高原生物研究所和中国科学院高原生物进化与适应重点实验室, 青海, 西宁, 810008

通讯作者: 汪诗平,E-mail:wangship2008@yahoo.cn
作者简介:刘贵河(1968- ),男,内蒙古太仆寺旗人,副教授,博士,主要从事草地资源、草地生态与管理方面研究,E-mail: guiheliu@sina.com
基金资助:
中国科学院知识创新工程方向性项目(KSCX2-SW-107);国家科技支撑计划项目(2007BAD52B06-2);京承路都市型现代农业走廊工程科技示范项目(D08060500460803)

Abstract

Abstract: In order to probe into the application of n-alkanes as markers for estimating diet composition and herbage intake of free grazing herbivores in rangeland,an experiment was carried out in summer and autumn 2004 at the Inner Mongolia Grassland Ecosystem Research Station of Chinese Academy of Sciences.Four cows selected from a group of 4 years old were grazed in rangeland from 1 July to 13 August to study their diet composition and herbage intake during two grazing periods.Artificial YC capsules were dosed in the beginning of each period.The samples of herbage and feces were collected daily for 7 days and dried for n-alkane concentration analysis using gas chromatography to estimate the feces output,diet composition,and herbage intake of cows.The results show that the plant species were eaten by grazing cows were similar,the diet composition were different in grazing seasons,and their herbage intake significantly increased with the proceeding of grazing seasons(P</i>< 0.05),but the feces output did not differ significantly(P> 0.05).In conclusion,n-alkane technique could provide an estimation of feces output,diet composition and herbage intake of grazing cows,and grazing seasons had more effect on their herbage intake.In order to improve n-alkane technique,other methods should be compared with n-alkane technique for the estimation of diet composition and herbage intake of free grazing herbivores in species mixed rangelands.

Key words: n-Alkane technique, Grazing cow, Diet composition, Herbage intake, Typical steppe

摘要： 为进一步探讨饱和链烷技术在典型草原自由放牧家畜中的应用,定量研究放牧牛食性及食量,于2004年夏秋季节在中国科学院内蒙古草原生态系统定位研究站典型草原进行自由放牧试验,选择体重相近4岁肉乳兼用哺乳牛4头,分两期测定其食性和食量,每头牛投喂一粒YC饱和链烷缓释胶囊,试验期内每天跟踪放牧牛的采食路线,收集其所采食的牧草及粪便样品,利用气相色谱分析牧草和粪样中的链烷含量,应用链烷技术估测放牧牛的排粪量、牧草采食比例和干物质采食量.结果表明:不同放牧季节放牧牛采食的牧草种类基本相同,但采食的比例存在差异,随放牧季节后移,放牧牛采食量显著增加(P<0.05),但日排粪量差异不显著.因此,链烷技术可以估测典型草原放牧牛的排粪量、食物组成和采食量,且放牧季节对放牧牛的采食量影响较大.建议在牧草种类组成较为复杂的典型草原估测放牧家畜食性食量时要进行多种方法比较,以进一步完善链烷技术.

关键词: 链烷技术, 放牧牛, 食性, 食量, 典型草原

CLC Number:

S823.4/.5

Cite this article

LIU Gui-he, WANG Shi-ping, ZHANG Ying-jun, HAN Jian-guo. Estimation of Diet Composition and Herbage Intake of Grazing Cows using n-Alkane Technique in Inner Mongolia Typical Steppes[J]. , 2009, 17(6): 693-698.

刘贵河, 汪诗平, 张英俊, 韩建国. 链烷技术估测典型草原放牧牛食性食量方法研究[J]. , 2009, 17(6): 693-698.

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Estimation of Diet Composition and Herbage Intake of Grazing Cows using n-Alkane Technique in Inner Mongolia Typical Steppes

链烷技术估测典型草原放牧牛食性食量方法研究

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[1]	SONG Jie, WANG Feng-ge, WEN Lu, WANG Li-xin, LI Jin-lei, WU Sheng-nan, XU Zhi-chao. Effects of Grazing on Plant Diversity and Soil Nutrients in Typical Temperate Steppe [J]. Acta Agrestia Sinica, 2019, 27(6): 1694-1701.
[2]	LI Meng-qi, HE Xing-dong, YANG Xiang-xiang, DING Xin-feng, ZHAO Yue-dan, GAO Yu-bao, YOU Wan-xue, YU Dian, CAI Li. Study on Calcium Oxalate in Plants of Two Types of Steppe Zones in Semi-arid Area [J]. Acta Agrestia Sinica, 2018, 26(6): 1421-1427.
[3]	HOU Dong-jie, QIAO Xian-guo, GAO Chen-guang, ZHAO Hai-wei, ZHAO Li-qing, GUO Ke. Hydro-ecological Effects of Litter in Typical Steppe of Inner Mongolia [J]. Acta Agrestia Sinica, 2018, 26(3): 559-565.
[4]	CHEN Xue-ping, YANG Yan-gang, SHANG Zhan-huan, CHEN Ji-ding, KONG Ya-ping, WANG Jiu-luan. A Study on the Characteristics of Soil Seed Bank of Grassland on Roadsides of Qinghai-Tibet Plateau-Take the Highway of G214 for Example [J]. Acta Agrestia Sinica, 2018, 26(1): 85-91.
[5]	WANG Xi, JIA Li-xin, QIAO Ji-rong, YANG Yang, ZHANG Feng, ZHAO Meng-Li. Effects of Stubble Height on Characteristics of Stipa grandis Populations in Typical Steppe [J]. Acta Agrestia Sinica, 2018, 26(1): 92-98.
[6]	LIU Gui-he, GE jian, LI jun, LIU Chun-yang, ZHANG Ying-jun, WANG Shi-ping. Study on the Diet Composition of Grazing Sheep in the Agro-pastoral Transition Zone of North China [J]. Acta Agrestia Sinica, 2017, 25(4): 743-748.
[7]	CAO Li-xia, HOU Wei-feng, QIAN Jie-xin, WU En. Effects of Mycorrhizal Inoculation on the Content of Main Nutrients and Glomalin in the Rhizosphere of Leymus Chinensis(Trin.) Tzvel. [J]. Acta Agrestia Sinica, 2016, 24(3): 537-543.
[8]	HU Jing, HOU Xiang-yang, SA Ru-la, LI Xi-liang, WANG Zhen, DING Yong. Regulatory Effects of Stip grandis on Above-ground Biomass of Plant Community in Grazing Ecosystem [J]. Acta Agrestia Sinica, 2016, 24(1): 1-11.
[9]	HAN Hui-ge, SHANG Zhen-yan, NIU De-cao, FU Hua. Effect of Nitrogen Addition on the Specific Leaf Area and Important Value of Dominant Species in the Typical Steppe of the Loess Plateau, China [J]. Acta Agrestia Sinica, 2015, 23(1): 69-74.
[10]	DING Yong, NIU Jian-ming, LIU Peng-tao, DONG Jian-jun, ZHANG Qing, Sarula. Dynamic Analyses of Household Spatial and Temporal Distribution Patterns in the Huanghuashute of Baiyinxile Ranch,Inner Mongolia [J]. Acta Agrestia Sinica, 2014, 22(6): 1205-1211.
[11]	ZHAO Xin-yu, LI Hong-hong, CHENG Ji-min, SU Ji-shuai, ZHU Ren-bin. Value Assessment of Typical Steppe Ecosystem in Yunwu Mountain National Nature Reserve [J]. , 2014, 22(2): 217-223.
[12]	LIU Gui-he, WANG Guo-jie, WANG Shi-ping, ZHANG Ying-jun, SHAO Xin-qing, WAN Xin-rong, HAO Shu-guang. Diet Composition and Trophic Niche of Main Herbivores in the Typical Steppe of Inner Mongolia [J]. , 2013, 21(3): 439-445.
[13]	WU Yan-qin, CHENG Ji-min, BAI Yu, ZHU Ren-bin, CHEN Ao, WEI Lin. Effects of Different Slope on Litter Decomposition Characteristics in the National Natural Reserve of Yunwu Mountain [J]. , 2013, 21(3): 460-466.
[14]	LUO Xiao-yan, JIAO Yan. Effects of Fencing Duration on CH₄ Uptake in Typical Steppe [J]. , 2012, 20(6): 1051-1058.
[15]	SHAN Gui-lian, XUE Shi-ming, CHEN Gong, KUANG Chong-yi, Liu Zhong-lin, CHU Xiao-hui. Influence of Seasonal Exclosure on Vegetation Restoration in Typical Steppe, Inner Mongolia [J]. , 2012, 20(5): 812-818.