The Pathogen of the Medicago stativa Leaf Spot in Gansu Province

doi:10.11733/j.issn.1007-0435.2010.03.011

›› 2010, Vol. 18 ›› Issue (3): 372-377.DOI: 10.11733/j.issn.1007-0435.2010.03.011

Previous Articles Next Articles

The Pathogen of the Medicago stativa Leaf Spot in Gansu Province

WANG Ting, WANG Sheng-rong

College of Prataculture, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem, Ministry of Education, Sino-U.S Centers for Grazingland Ecosystem Sustainability, Lanzhou, Gansu Province, 730070, China

Received:2009-11-30 Revised:2010-04-06 Online:2010-06-15 Published:2010-06-15

甘肃苜蓿斑点病的初步研究

王婷, 王生荣

甘肃农业大学草业学院, 草地生态系统教育部重点实验室, 中美草地畜牧业可持续发展研究中心, 甘肃, 兰州, 730070

通讯作者: 王生荣,E-mail:wangsr@gsau.edu.cn
作者简介:王婷(1982- ),女,汉族,甘肃临洮人,硕士研究生,研究方向为植物病理学,E-mail:lz_wangt@163.com
基金资助:
教育部博士点基金(20070733003)资助

Abstract

Abstract: Leaf spot is a serious leaf disease in alfalfa,which have been in Gansu Province in recent years.The disease reduced yield and forage value of alfalfa.In order to effectively control the disease of alfalfa,the morphology and biological characteristics of the pathogen was made in laboratory.The results indicated that it was caused by Alternaria alternata(Fr.:Fr) Keissler.The Pathogen can utilize sucrose,maltose,glycin Well.The optimum temperature,pH value and relative humidity(RH) of the pathogen are 25℃,6.0-7.0,above 98% respectively.Under such conditions the pathogens grow quickly,the aerial mycelium of colony is abundant,the conidia germinate well.

Key words: Medicago stativa, Leaf spot, Alternaria alternate, Biological characters

摘要： 近年来甘肃省苜蓿(Medicago stativa L.)发生一种叶部病害—-斑点病,严重地降低了苜蓿的产量和饲用价值。为了有效地控制该病害对苜蓿的危害,在室内对其病原菌进行了形态学鉴定及生物学特性研究。结果表明:苜蓿斑点病的病原菌为链格孢菌(Alternaria alternata (Fr.:Fr) Keissler),该病原菌能很好地利用麦芽糖、蔗糖、甘氨酸;其生长最适温度为25℃,最适pH为6.0-7.0,最适宜的RH为98%以上,最适培养基为5%AL和PSA。在上述环境条件下,菌丝生长快,菌丝茂盛,产孢量大,孢子萌发率高。

关键词: 苜蓿, 斑点病, 链格孢菌, 生物学特性

CLC Number:

S431.14

WANG Ting, WANG Sheng-rong. The Pathogen of the Medicago stativa Leaf Spot in Gansu Province[J]. , 2010, 18(3): 372-377.

王婷, 王生荣. 甘肃苜蓿斑点病的初步研究[J]. , 2010, 18(3): 372-377.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2010.03.011

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2010/V18/I3/372

References

[1] 耿华珠.中国苜蓿[M]. 北京:中国农业出版社,1995:114-130
[2] 袁庆华.我国苜蓿病害研究进展[J]. 植物保护,2007,1(33):6-11
[3] 南志标.建立中国的牧草病害可持续管理体系[J]. 草业学报,2000,9(2):19
[4] 南志标,李春杰.中国牧草真菌病害名录[J]. 草业科学,1994,(增刊):1-160
[5] Hollingsworth C R,Gray F A,Koch D W.Alfalfa Disease Management[M]. Wyoming:College of Agriculture,2003,13-22
[6] Donald L S,Donald C E.Compedium of Alfalfa Disease[M]. The American Phytopathological Society Press.1990,13-47
[7] 方仲达.植病研究法[M]. 第3版,北京:中国农业出版社,1998,12:122-125
[8] 雷玉明,张建文.草本植物三种真菌新病害[J]. 草地学报,2006,14(3):284-286
[9] 刘博,傅俊范.五味子叶枯病病原菌鉴定[J]. 植物病理学报,2008,38(4):425-428
[10] Allen S J,Brown J F,Kochman J K.Effects of temperature dew period and light on the growth and development of Alternaria helianthi[J]. Phytopathology,1983,73:893-896
[11] 郑莉,黄俊斌,向培建,等.高羊茅叶斑病病原鉴定及生物学特性[J]. 草地学报,2006,14(4):315-318
[12] 张天宇.中国真菌志链格孢属[M]. 北京:科学出版社,2003,38-42
[13] 戚佩坤.吉林经济植物真菌病害志[M]. 辽宁:吉林人民出版社,1965,234-238
[14] 贾菊生,胡守智.新疆经济植物真菌病害志[M]. 新疆:新疆科技卫生出版社,1994,73-81
[15] 南志标.陇东黄土高原栽培牧草真菌病害调查与分析[J]. 草业科学,1990,7(4):30-34
[16] 李春杰,南志标.苜蓿种带真菌及其致病性测定[J]. 草业学报,2000,9(1):27-36
[17] 奚启新.苜蓿种子检测及处理分析[J]. 东北农业大学学报,2000,31(1):43-48
[18] 郭玉霞,南志标,李春杰,等.黄土高原区苜蓿与小麦轮作系统根部入侵真菌研究[J]. 生态学报,2004,24(3):486-494
[19] Naseri B,Marefat A R.Seasonal dynamics and prevalence of alfalfa fungal pathogens in Zanjan province,Iran[J]. International Journal of Plant Production,2008,2(4):327-340
[20] Undi M,Wittenberg K M,Holliday N J.Occurrence of fungal species in stored alfalfa forage as influenced by moisture content at baling and temperature during storage[J]. Canadian Journal of Animal Science,1997,77(1):95-103
[21] Wilczek M,Koper R,′Cwintal M.Germination capacity and health status of hybrid alfalfa seeds after laser treatment[J]. International Agrophysics,2005,19:257-261
[22] Bijeli ′c Z,Krnjaja V,Tomi ′c Z.Identification and quantification of fungi in grass-leguminous silage[J]. Biotechnology in Animal Husbandry,2009,25(5-6):1209-1216
[23] 詹文平,朱建兰,李晓玲.红豆草黑斑病病原生物学特性及产毒与侵染条件的研究[J]. 草原与草坪,2008,9:54-58
[24] 孙仲平,马文海,王占斌.杨叶枯病病原菌生物学特性初步研究[J]. 中国森林病害,2007,26(1):4-7
[25] 王宏.梨黑斑病菌生物学特性及其生物-化学协同控制技术的研究[D] :[学位论文]. 南京:南京农业大学,2006,25-42
[26] 郭霞,黄晓亚.红豆草黑腐病菌菌丝生长和产毒培养条件的优化[J]. 草地学报,2009,17(2):255-258

The Pathogen of the Medicago stativa Leaf Spot in Gansu Province

甘肃苜蓿斑点病的初步研究

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 13

Recommended Articles

Metrics

Comments

[1]	NIE Xiu-mei, ZHAO Gui-qin, SUN Hao-yang, CHAI Ji-kuan, WU Wen-bin, LIN Long, SUN Lei-lei, WANG Jun, WANG Miao-miao, KANG Xiao-qiang. Occurrence and Pathogenic Identification of Oat Leaf Spot in Tianzhu County [J]. Acta Agrestia Sinica, 2019, 27(5): 1384-1391.
[2]	FANG Ting, SU Hao-tian, YIN Shu-xia. Isolation and Identification of Alfalfa Leaf Spot Pathogen and Resistance Screening of Medicago sativa Varieties [J]. Acta Agrestia Sinica, 2018, 26(3): 697-704.
[3]	ZHANG Jia-qi, ZHAO Yun-meng, HAN Zhi-yong, ZHAO Mei-qi. Identification and Control of Turf Gray Leaf Spot [J]. Acta Agrestia Sinica, 2017, 25(2): 442-444.
[4]	ZHANG Li, PAN Long-qi, YUAN Qing-hua, WANG Yu, MIAO Li-hong. Resistance Evaluation of Different Alfalfa Materials on Phoma Leaf Spot [J]. Acta Agrestia Sinica, 2016, 24(3): 652-657.
[5]	LI Hong-jian, LI Fang-hong, SHI Jian, Hu Tian-ming, Cheng Ji-ming, QIAN Yong-hua, JIAO Feng. Identification of Pathogen Causing Phoma Leaf Spot on Medicago sativa in Shaanxi Province [J]. Acta Agrestia Sinica, 2015, 23(6): 1310-1316.
[6]	BI Bo, WANG Yu, YUAN Qing-hua, GAO Jian-ming. Verification of Molecular Markers Linked to Resistance Gene of Common Leaf Spot Disease in Tetraploid Alfalfa [J]. , 2011, 19(4): 663-667.
[7]	MENG Fang, YUAN Qing-hua, SU De-rong, GAO Jian-ming. Study on ISSR Markers Linked to Common Leaf Spot Disease Resistance Gene of Tetraploid Alfalfa [J]. , 2007, 15(6): 561-565.
[8]	YUAN Qing-hua, MA Hong-wen, XU Bing-liang. Pathogenic Differentiation of Pseudopeziza medicaginis [J]. , 2007, 15(4): 312-315.
[9]	ZHENG Li, HUANG Jun-bin, Hsiang Tom. Etiological and Biological Characterization of the Pathogenic Fungus Causing Leaf Spot on Festuca arundinacea [J]. , 2006, 14(4): 315-318,327.
[10]	DING Cheng-long, SHEN Yi-xin, GU Hong-ru, Yuichi Miura. Identification of an EST-CAPS Marker Linking to the Resistance Gene of Italian Ryegrass Gray-Leaf-Spot Disease by Bulked Segregant Analysis [J]. , 2006, 14(1): 9-13,23.
[11]	HOU Jian-hua, YUN Jin-feng. Biological Characters of Leymus Chinensis and Leymus Cinereus and Their Hybrid [J]. , 2005, 13(3): 175-179.
[12]	ZHONG Xiao-xian, GU Hong-ru, DING Cheng-long, BAI Su-juan, XIANG Yang-hai, LIU Zhao-hui . Study on Sorghum sudanese × S.propinguum interspecies hybridization [J]. , 2002, 10(1): 24-27.
[13]	YUAN Qing-hua, ZHANG Wen-shu, LI Min . Study on Excised Leaf Tissue Inoculation of Common Leaf Spot in Alfalfa [J]. , 2001, 9(1): 21-24.