Abstract: Five kinds of alfalfa cell culture lines are established to explore different genetic cells and their culture methods to produce different protein contents and biomass.Result showed that five types of alfalfa cells produced the highest protein content at the 18^th day and the highest biomass at 9^th to 12^th day.The highest protein content of different alfalfa cultivars ranked as Alfalfa King>Algonquin>Debao> DeliIII>Englhard.They show a range of nitrogen metabolism reactions due to NH₄⁺/NO₃^- and may be ranked as Alfalfa King>Englhard,Debao>Algonquin and DeliIII.The optimum carbon content for alfalfa cells growth and osmotic pressure is 2%-3% sucrose.DeliIII and Englhard cells show best growth in 3% sucrose content.This article provides a database for further study of a large-scale production of protein in cultured alfalfa cells.

Key words: Alfalfa cell, Protein content, Biomass, Culture procedure

摘要： 通过对5种苜蓿(Medicago sativa L.)细胞培养物和细胞系的建立,探讨亲缘关系较远的5种苜蓿细胞在培养过程中,蛋白质和生物量的变化以及在不同氮源和不同蔗糖量为碳源的环境中蛋白质和生物量的变化,为大规模培养苜蓿细胞和细胞株的筛选提供一定的基础。结果表明:5种苜蓿细胞在培养过程中均在第18 d达到最大蛋白质含量,而生物量则在第9~12 d达到最大,此时5种苜蓿细胞的最大蛋白质含量不同,分别是苜蓿王>阿尔冈金>德宝>三德利>安格;由于NH₄⁺/NO₃^-比例不同,使5种苜蓿细胞在生长过程中对氮的代谢反应表现出不同的适应性。苜蓿王、安格和德宝的细胞对氮源的适应性比阿尔冈金和三德利的细胞范围广;2%~3%的蔗糖含量基本满足5种苜蓿细胞的生长并能维持渗透压的稳定,但不同品种苜蓿细胞对蔗糖浓度的选择稍有不同,如三德利和安格更适合于3%的蔗糖浓度。

关键词: 苜蓿细胞, 蛋白质含量, 生物量, 培养过程