In Vitro Tip Micropropagation of Ligularia jamesii from Changbai Mountain

doi:10.11733/j.issn.1007-0435.2011.02.019

›› 2011, Vol. 19 ›› Issue (2): 283-287.DOI: 10.11733/j.issn.1007-0435.2011.02.019

Previous Articles Next Articles

In Vitro Tip Micropropagation of Ligularia jamesii from Changbai Mountain

ZHUANG Qian-qian¹, LI Wen-xin², GU De-feng¹, CHEN Shao-peng¹, DONG Ran¹

1. College of Horticulture, Jilin Agricultural University, Changchun, Jilin Province 130118, China;
2. Huang Nihe forestry bureau of Jilin Province, Dunhua, Jilin Province 133704, China

Received:2010-09-26 Revised:2011-02-17 Online:2011-04-15 Published:2011-04-15

长白山单花橐吾茎尖离体培养研究

庄倩倩¹, 李文鑫², 顾德峰¹, 陈少鹏¹, 董然¹

1. 吉林农业大学园艺学院, 吉林, 长春, 130118;
2. 吉林省黄泥河林业局, 吉林, 敦化, 133704

通讯作者: 董然,E-mail:Dongr999@163.com
作者简介:庄倩倩(1984- ),女,吉林省临江市人,硕士研究生,研究方向为野生植物引种驯化,E-mail:zhuangqian_0156@sina.com
基金资助:
吉林省科学技术厅-长白山特种经济植物保存及配套关键技术研究(20100259)资助

Abstract

Abstract: Stem tips of Ligularia jamesii were used as explants to induce plumule proliferation.Results showed that the best combinations inducing plumules are MS medium supplemented with 6-BA(3.0 mg·L^-1) and NAA(0.3 mg·L^-1).During the initial culture,the induction rate reached 90.1%.MS medium supplemented with 6-BA(2.5 mg·L^-1) and IBA(0.1 mg·L^-1) is used for propagation subculture,while propagation coefficient is 4.9 and the plantlets grew vigorously.For rooting culture,1/2MS medium with NAA(0.5 mg·L^-1) gave best results with white roots well developed and 95% rooting rate.

Key words: Ligularia jamesii, Stem tip, Micropropagation

摘要： 以单花橐吾(Ligularia jamesii)茎尖作为外植体进行幼芽诱导,探索单花橐吾的离体快繁途径。结果表明:在MS+6-BA(3.0 mg·L^-1)+NAA(0.3 mg·L^-1)+蔗糖(35 g·L^-1)培养基上的幼芽诱导效果最好,诱导率高达90.1%;增殖培养时在MS+6-BA(2.5 mg·L^-1)+IBA(0.1 mg·L^-1)培养基上的平均增殖系数4.9,幼芽生长健壮,可继代培养6次以上;生根培养采用1/2MS+NAA(0.5 mg·L^-1),生根率达95%,生根白色粗壮,且数量较多。

关键词: 单花橐吾, 茎尖, 快繁

CLC Number:

Q943.1

ZHUANG Qian-qian, LI Wen-xin, GU De-feng, CHEN Shao-peng, DONG Ran. In Vitro Tip Micropropagation of Ligularia jamesii from Changbai Mountain[J]. , 2011, 19(2): 283-287.

庄倩倩, 李文鑫, 顾德峰, 陈少鹏, 董然. 长白山单花橐吾茎尖离体培养研究[J]. , 2011, 19(2): 283-287.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2011.02.019

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2011/V19/I2/283

References

[1] 中国科学院中国植物志编辑委员会.中国植物志.第77卷[M].北京:科学出版社,1999
[2] 董然.中国长白山橐吾属植物种质资源研究[D].长春:吉林农业大学,2009
[3] 付沛云.东北植物检索表[M].北京:科学出版社,1995.685
[4] 严仲铠,李万林.中国长白山药用植物彩色图志[M].北京:人民卫生出版社,1997.441-442
[5] 周繇.长白山区珍稀濒危观赏植物优先保护定量研究[J].武汉植物学研究,2006,24(4):357-364
[6] 董然,南敏仑,刘洪章.长白山特有种单头橐吾的挥发油成分研究[J].安徽农业科学,2009,37(36):17900-17901
[7] Hajime Naganoa,Atsushi Torihatab,Mika Matsushimaa,et al.Chemical and genetic study of Ligularia cyathiceps in Yunnan Province of China[J].Helvetica Chimica Acta,2009,92:2071-2081
[8] Motoo Tori,Aki Watanabea,Sachie Matsuo.Diversity of Ligularia kanaitzensis in sesquiterpenoid composition and neutral DNA sequences[J].Tetrahedron,2008,64:4486-4495
[9] 吴迪瑶.黄亮橐吾化学成分研究及糙叶大头橐吾组织培养方法研究[D].浙江:浙江大学,2006
[10] 杜娟,杨柏明,李彦舫.蹄叶橐吾的组织培养及植株再生[J].植物生理学通讯,2006,42(4):687
[11] 卢婷,林夏珍.蹄叶囊吾和窄头橐吾的引种培育及抗性研究[D].浙江:浙江林学院,2008
[12] 董然,潘艳艳,王丽清,等.复序橐吾组培再生体系研究[J].草地学报,2010,18(2):286-290
[13] 刘珍,袁庆华,王瑜.等.多年生黑麦草高频丛生芽增殖培养体系的研究[J].草地学报,2010,18(4):576-583
[14] 宋俊双,王赞,孙桂芝,等.柠条锦鸡儿的组织培养[J].草地学报,2007,15(1):66-69
[15] 鲍芫,米福贵.长穗偃麦草与中间偃麦草杂种成熟胚离体培养与再生体系的建立[J].草地学报,2007,15(4):348-351
[16] 郭晔红,蔺海明,武睿,等.唐古特白刺组织培养及其培养基筛选研究[J].草业学报,2009,18(6):59-64
[17] 赵智燕,潘俊松,何亚丽,等.两个高羊茅无性系的营养器官组织培养及再生体系的建立[J].革业学报,2009,18(5):168-175
[18] 于娜,董宽虎.羊草成熟胚组织培养及植株再生体系的建立[J].草地学报,2008,16(5):466-469
[19] 厚彦明,师尚礼,杜文华,等.岷山红三叶愈伤组织诱导和植株再生研究[J].草地学报,2008,16(4):359-363
[20] 于林清,何茂泰,王照兰.甘草组织培养快速繁殖技术研究[J].中国草地,1999,21(1):12-14,18

In Vitro Tip Micropropagation of Ligularia jamesii from Changbai Mountain

长白山单花橐吾茎尖离体培养研究

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHAO Jiao-yang, LI Xiao-yan, ZHU Hui-sen, ZHANG Shi-min, ZHANG Yan. Seed Germination and Plant Regeneration System Optimization of Medicago sativa ‘Pianguan’ [J]. Acta Agrestia Sinica, 2020, 28(4): 947-955.
[2]	HU Yan-ping, WANG Li, BAO Rui, SHI Lin, XU Rong-hua, LI Yi. Optimizing ISSR-PCR System of Carex atrofusca Using Orthogonal Design [J]. Acta Agrestia Sinica, 2014, 22(4): 911-914,918.
[3]	LIU Yan-rong, ZHANG Wan-jun. Evaluation of the Tissue Culture Responses of 11 Switchgrass Varieties from Different Ecotypes [J]. Acta Agrestia Sinica, 2014, 22(3): 579-585.
[4]	ZHANG Li-jun, LIU Long-long, QIAO Zhi-jun, MA Ming-chuan, ZHOU Jian-ping, FAN Yin-yan, CUI Lin. Transcriptome Sequencing and Analysis of Oat Nuclear Sterile Stamens [J]. , 2014, 22(2): 352-358.
[5]	ZHANG Yu, BAI Shi-qie, LI Da-xu. Effects of Different Culture Media on the Leaf Regeneration of Chicory [J]. , 2014, 22(1): 208-212.
[6]	LU Yu-fei, JIANG Jian-xiong, YI Zi-li. Optimization the ISSR-PCR Amplification Reaction System of Miscanthus from China [J]. , 2013, 21(1): 167-173.
[7]	LIU Wen-ting, LIU Yong-hong, HE Wei-juan, DUAN Qi-mei, QUAN You. Desiccation Somatic Embryos of Transgenic Alfalfa [J]. , 2012, 20(6): 1143-1149.
[8]	ZHAO Yong-qin, ZHANG Na, WU Xin-xin, ZHENG He, LI Hong-bo, ZHAO Bing, GUO Yang-dong. Regeneration Systems for in Vitro Culture of Filament and Genetic Variation Analyses of Yucca gloriosa L. [J]. , 2012, 20(5): 921-926.
[9]	LIU Fang, WANG Yu-xiang, CHEN Ai-ping, ZHANG Bo. Callus Induction and Differentiation of Xinjiang Medicago falcata L. [J]. , 2012, 20(4): 741-746.
[10]	HOU Yong-xia, WANG Jun-jie, ZHAO Yan, YUN Jin-feng, CHEN Xue-ying. Tissue Culture and Regeneration System of Native Medicago falcata L. [J]. Acta Agrestia Sinica, 2012, 20(3): 524-529.
[11]	LI Yu-zhu, SHI Shang-li, HE Yan-yu. Effects of Different Fluorescent Dyes on Staining Alfalfa Protoplast [J]. , 2012, 20(2): 348-351.
[12]	LIU Ming-xi, GUO Zhen-fei. Effects of Different Sterilization Methods on Callus Induction of Centipedegrass (Eremochloa ophiuroides) [J]. , 2012, 20(2): 383-388.
[13]	GAO Xue-qin, WANG Jun-jie, YUN Jin-feng, HOU Yong-xia, WANG Ming-tao, ZHANG Yuan-ke. Tissue Culture and Regeneration of Red Clover New Line [J]. , 2012, 20(1): 159-165.
[14]	ZHANG Li-jun, CHENG Lin-mei, DU Jian-zhong, LI Gui-quan, SUN Yi. Establishment and Optimization of Puna Chicory Genetic Transformation System with Agrobacterium-mediated Method [J]. , 2011, 19(6): 1042-1050.
[15]	TAO Rong, LI Yu-zhu, WANG Juan, AN Hui-hui, GUO Jing, SHI Shang-li. Protoplasts Isolation Conditions of Melilotoides ruthenica Callus [J]. , 2011, 19(2): 288-293.