Acta Agrestia Sinica

›› 2012, Vol. 20 ›› Issue (2): 383-388.DOI: 10.11733/j.issn.1007-0435.2012.02.031

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Effects of Different Sterilization Methods on Callus Induction of Centipedegrass (Eremochloa ophiuroides)

LIU Ming-xi1,2, GUO Zhen-fei2,3   

  1. 1. Hunan Agricultural University, Changsha, Hunan Province 410128, China;
    2. College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong Province 518020, China;
    3. Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou, Guangdong Province 510642, China
  • Received:2011-11-21 Revised:2012-01-06 Online:2012-04-15 Published:2012-07-05

不同消毒方式对假俭草种子愈伤诱导的影响

刘明稀1,2, 郭振飞2,3   

  1. 1. 湖南农业大学, 湖南 长沙 410128;
    2. 华南农业大学生命科学学院, 广东 广州 510642;
    3. 华南农业大学广东省森林植物种质创新与利用重点实验室, 广东 广州 510642
  • 通讯作者: 郭振飞,E-mail:zhfguo@scau.edu.cn
  • 作者简介:刘明稀(1975- ),男,湖南新化人,博士,讲师,主要从事草坪草、牧草及能源草生物技术育种,E-mail:lmx751221@163.com
  • 基金资助:
    广东省科技计划项目(2006B20201035);湖南农业大学校青年科学基金(2010QN30)资助

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Abstract: An efficient regeneration system is crucial to crop breeding by biotechnology. Explant sterilization methods are key factors for an efficient regeneration system. Eight sterilization methods (A1:3.5% sodium hypochlorite 25 min, A2:concentrated sulphuric acid 30 s+3.5% sodium hypochlorite 20 min, B1:7% sodium hypochlorite 20 min, B2:concentrated sulphuric acid 30 s+7% sodium hypochlorite 20 min, C1:0.05% mercury bichloride 20 min, C2:mercury bichloride 30 s+0.05% mercury bichloride 15 min, D1:0.1% mercury bichloride 15 min, D2:mercury bichloride 30 s+0.1% mercury bichloride 10 min) were tested to determine an efficient regeneration system for Centipede grass. Results showed the A2 treatment with 52% callus induction ratio as the best for Centipede grass, whereas the D1 treatment resulted in the lowest callus induction ratio (17.33%). Seeds treated by concentrated sulfuric acid with 30 s had a higher callus induction ratio than those not treated with concentrated sulfuric acid. Results also indicated that sodium hypochlorite was more efficient than mercury bichloride for Centipede grass callus induction.

Key words: Centipedegrass, Sterilization methods, Callus induction

摘要： 为确定假俭草(Eremochloa ophiuroides)再生体系中种子外植体的消毒方式,经过预备试验后共设计A,B,C,D 共4组2系列8种消毒方式。A1:3.5%次氯酸钠消毒25 min,A2:浓硫酸灼烧30 s+3.5%次氯酸钠消毒20 min,B1:7%次氯酸钠消毒20 min,B2:浓硫酸灼烧30 s+7%次氯酸钠消毒20 min,C1:0.05%升汞消毒20 min,C2:浓硫酸灼烧30 s+ 0.05%升汞消毒15 min,D1:0.1%升汞消毒15 min,D2:浓硫酸灼烧30 s+ 0.1%升汞消毒10 min。结果表明:A2的愈伤诱导效果最好,达到52%;D1的愈伤诱导率最低,仅17.33%,其他处理的愈伤诱导率介于二者之间。发现浓硫酸灼烧假俭草种子30 s,可显著提高出愈率;试验证实消毒剂次氯酸钠比升汞有利于假俭草种子的愈伤诱导。

关键词: 假俭草, 消毒方式, 愈伤诱导

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