Abstract: The ultrastructures of halophytes Artemisia anethifolia leaf and stem were studied using transmission electron microscope.Results showed that various organelles of mesophyll and stem maintained morphological integrity under normal condition,while their ultrastructure changed under 200mmol/L NaCl stress.In mesophyll cells,one part of chloroplasts remained normal.The destroyed part of chloroplast swelled and thylakoids were blurred.The numbers of mitochondria increased but with crista reducing.There were few vacuolar inclusions and transfer cells in leaf tissue.The chloroplast was similar to the mesophyll cells in stem cells but with more and bigger starch grains.The numbers of mitochondrion were also increased.There were crystals in cytoplasm and contents in vacuole.These results indicated that salt stress cause severe damages to the ultrastructure of Artemisia anethifolia,but halophytes had some structural characteristics that adapt to salt stress.

Key words: Halophytes, Artemisia anethifolia, NaCl stress, Mesophyll cells, Stem cell, Ultrastructure

摘要： 为研究盐胁迫对盐生植物碱蒿(Artemisia anethifolia)超微结构的影响,用透射电子显微镜对NaCl胁迫下碱蒿的叶片和茎进行了观察与研究。结果表明:未胁迫的碱蒿叶片及茎细胞中各细胞器结构完整。200 mmol·L^-1的NaCl胁迫,引起碱蒿超微结构的变化,叶片中部分叶绿体结构正常,部分被破坏的叶绿体肿胀变形,类囊体模糊不清;线粒体数增多,嵴减少甚至模糊;液泡中出现内含物,叶肉细胞中出现传递细胞。碱蒿茎中叶绿体与叶片类似,另外,茎中含有较多大的淀粉粒;线粒体数增多;细胞质中有晶体存在;其液泡中有内含物。这些结果表明虽然盐胁迫对植物超微结构有严重的破坏作用,但盐生植物碱蒿存在适应盐胁迫的结构特征。

关键词: 盐生植物, 碱蒿, NaCl胁迫, 叶肉细胞, 茎细胞, 超微结构