Acta Agrestia Sinica ›› 2021, Vol. 29 ›› Issue (6): 1363-1368.DOI: 10.11733/j.issn.1007-0435.2021.06.029

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Effects of 2,4-table Brassinolide on Enzyme Activity and Root ion Distribution and Absorption in Alfalfa Seedlings

WANG Wen-jing, MA Dong-mei, ZHAO Li-juan, MA Qiao-li   

  1. State Key Laboratory for Land Degradation and Ecosystem Restoration in Northwest China, Ningxia University/Key Laboratory for Ecosystem Restoration and Reconstruction in Northwest China, Ministry of Education, Ningxia University, Yinchuan, Ningxia 750021, China
  • Received:2020-08-12 Revised:2020-09-15 Online:2021-06-15 Published:2021-07-05

2,4-表油菜素内酯对盐胁迫下紫花苜蓿生理指标及根系离子积累的影响

王文静, 麻冬梅, 赵丽娟, 马巧利   

  1. 宁夏大学西北土地退化与生态系统恢复省部共建国家重点实验室培育基地/西北退化生态系统恢复与重建 教育部重点实验室, 宁夏 银川 750021
  • 通讯作者: 麻冬梅,E-mail:576494584@qq.com
  • 作者简介:王文静(1994-),女,甘肃会宁人,硕士研究生,主要从事牧草分子育种研究,E-mail:1961224619@qq.com
  • 基金资助:
    国家自然科学基金项目(31760698);宁夏重点研发项目(2019BBF02022-04);宁夏高等学校一流学科建设(生态学)项目(NXYLXK2017B06)资助

Abstract: This study was aimed to investigate the effects of 2,4-epibrassinolide (EBR) on physiological indexes and root ion accumulation of Medicago sativa under salt stress in artificial climate chamber. The results showed that 150 mmol·L-1 salt stress significantly inhibited the growth of alfalfa. When the concentration of exogenous hormone EBR was 0.6 μmol·L-1 (M2),the activities of superoxide dismutase (SOD),catalase (CAT),peroxidase (POD) and soluble protein content were significantly increased,and malondialdehyde (MDA) was decreased,The results showed that 0.6 μmol·L-1 (M2) and 1.2 μmol·L-1 (M3) treatments significantly reduced the toxicity of Na+ to K+,Ca2+,Mg2+ and controlled the ion balance;therefore,exogenous hormone EBR could reduce the damage of salt stress to roots and enhance the activity of root antioxidant system.

Key words: 2,4-brassinolactone, Alfalfa, NaCl stress, Physiological characteristics, Ion metabolism

摘要: 为探讨2,4-表油菜素内酯(2,4-Epibrassinolide,EBR)对紫花苜蓿受盐胁迫的影响,本试验以紫花苜蓿‘阿迪娜’为材料,采用人工气候室水培试验,研究盐胁迫条件下喷施不同浓度的外源激素EBR对紫花苜蓿生理指标和根系离子积累的作用。结果表明:150 mmol·L-1盐胁迫对苜蓿生长具有明显的抑制作用,当喷施外源激素EBR浓度为0.6 μmol·L-1时,可显著提高超氧化物歧化酶(Superoxide dismutase,SOD)、过氧化氢酶(Catalase,CAT)、过氧化物酶(Peroxidase,POD)活性和可溶性蛋白含量,降低丙二醛(Malondialdehyde,MDA)含量和电解质渗透率;0.6 μmol·L-1和1.2 μmol·L-1EBR处理显著减轻Na+对K+,Ca2+,Mg2+的毒害,控制离子平衡。由此可见,外源激素EBR可以减小盐胁迫对根系的伤害,增强根系抗氧化系统活性。

关键词: 2,4-表油菜素内酯, 紫花苜蓿, NaCl胁迫, 生理特性, 离子代谢

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