The Effects of Homofermentative Lactobacillus on Ensiling Characteristics and in Vitro Digestibility of Low-moisture Alfalfa Silage

doi:10.11733/j.issn.1007-0435.2021.04.026

Acta Agrestia Sinica ›› 2021, Vol. 29 ›› Issue (4): 835-841.DOI: 10.11733/j.issn.1007-0435.2021.04.026

The Effects of Homofermentative Lactobacillus on Ensiling Characteristics and in Vitro Digestibility of Low-moisture Alfalfa Silage

HOU Hong-yan, SHEN Chen, HUO Wen-jie, LIU Qiang, ZHANG Shuan-lin, WANG Cong, CHEN Lei, XU Qing-fang, WANG Yong-xin, WANG Zhong-hao, GUO Gang^*

College of Animal Sciences, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China

Received:2020-11-06 Revised:2020-12-11 Published:2021-06-02
Contact: * E-mail:guosteel1984@126.com

同型发酵乳杆菌对低水分紫花苜蓿青贮发酵品质及体外消化率的影响

侯红雁, 申晨, 霍文婕, 刘强, 张拴林, 王聪, 陈雷, 许庆方, 王永新, 王忠豪, 郭刚^*

山西农业大学动物科学学院, 山西太谷 030801

通讯作者: * E-mail:guosteel1984@126.com
作者简介:侯红雁(1995-),女,山西太原人,硕士研究生,主要从事反刍动物营养与饲料科学研究,E-mail:804662423@qq.com
基金资助:
国家自然科学基金(32001405);山西省高等学校科技创新项目(2019L0356);山西农业大学引进人才科研启动项目(2014YJ11)资助

Abstract

Abstract: The objective of this study was to determine the effect of homofermentative lactobacillus on the fermentation quality and in vitro digestibility of low-moisture alfalfa (Medicago sativa) silage. Single factor treatments were designed with Control (C),and Lactobacillus coryniformis (LCO),Lactobacillus casei (LCA),Lactobacillus plantarum (LP) and Lactobacillus pentose (LPE) addition. Alfalfa was harvested at the early flowering stage and dried in the field (5 h) until the dry matter content was about 50%,then the silages were prepared. After 60 days ensiling,the silage fermentation quality was determined,their nutritional value was evaluated based on cornell net carbohydrate and protein system (CNCPS) and digestibility was evaluated by in vitro gas production method. The results showed that the pH value of LPE group was significantly lower than that of other groups (P<0.05). Compared with the Control,the content of NH₃-N in LCO,LCA and LPE groups decreased (P<0.05);and the content of non-protein nitrogen in all lactobacillus groups decreased (P<0.05);the content of rapid degradation protein and intermediately degradable protein in LP and LPE groups increased (P<0.05) while the contents of rapidly degradable carbohydrates in LP and LCA groups decreased (P<0.05). In in vitro fermentation test,the gas production,dry matter digestibility and neutral detergent fiber digestibility of silage in LPE group were higher than those of control (P < 0.05). Therefore,adding L. pentose to low moisture alfalfa silage (with 50% dry matter content) could improve silage fermentation quality,nutritional value and in vitro digestibility.

Key words: Alfalfa, Lactobacillus, Fermentation characteristics, Nutritional value

摘要： 为研究同型发酵乳杆菌对低水分紫花苜蓿(Medicago sativa)青贮发酵品质及体外消化的影响,本试验以田间晾晒5 h后的紫花苜蓿(干物质含量为50%)为原料,添加不同种类同型发酵乳杆菌[棒状乳杆菌(LCO)、干酪乳杆菌(LCA)、植物乳杆菌(LP)和戊糖乳杆菌(LPE)],调制青贮饲料,青贮60 d后,测定其发酵品质,基于康奈尔净碳水化合物-蛋白质体系评价其营养价值,并采用体外产气法评价其消化效率。结果表明:LPE处理组青贮饲料pH值显著低于其他各组(P<0.05)。与对照组相比,LCO,LCA和LPE处理组氨态氮含量均显著降低(P<0.05),各处理组的非蛋白氮含量均显著降低(P<0.05),LP和LPE处理组快速降解蛋白和中速降解蛋白含量均显著升高(P<0.05),而LP和LCA组快速降解碳水化合物显著降低(P<0.05)。体外发酵试验中,LPE组青贮饲料产气量、干物质消化率和中性洗涤纤维消化率均显著高于对照组(P<0.05)。因此,干物质含量为50%的低水分紫花苜蓿青贮饲料中,添加戊糖乳杆菌能够提高其发酵品质、营养价值及体外消化效率。

关键词: 紫花苜蓿, 乳杆菌, 发酵品质, 营养价值

CLC Number:

S541.9

HOU Hong-yan, SHEN Chen, HUO Wen-jie, LIU Qiang, ZHANG Shuan-lin, WANG Cong, CHEN Lei, XU Qing-fang, WANG Yong-xin, WANG Zhong-hao, GUO Gang. The Effects of Homofermentative Lactobacillus on Ensiling Characteristics and in Vitro Digestibility of Low-moisture Alfalfa Silage[J]. Acta Agrestia Sinica, 2021, 29(4): 835-841.

侯红雁, 申晨, 霍文婕, 刘强, 张拴林, 王聪, 陈雷, 许庆方, 王永新, 王忠豪, 郭刚. 同型发酵乳杆菌对低水分紫花苜蓿青贮发酵品质及体外消化率的影响[J]. 草地学报, 2021, 29(4): 835-841.

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The Effects of Homofermentative Lactobacillus on Ensiling Characteristics and in Vitro Digestibility of Low-moisture Alfalfa Silage

同型发酵乳杆菌对低水分紫花苜蓿青贮发酵品质及体外消化率的影响

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