Cloning and Expression Analysis of Dehydrin Gene from Agropyron cristatum(L.) Gaertn

doi:10.11733/j.issn.1007-0435.2012.01.022

›› 2012, Vol. 20 ›› Issue (1): 139-145.DOI: 10.11733/j.issn.1007-0435.2012.01.022

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Cloning and Expression Analysis of Dehydrin Gene from Agropyron cristatum(L.) Gaertn

ZHANG Nan, ZHANG Lin-sheng, XING Yuan, LIU Lan, LI Jia-hong

State Key Laboratory of Crop Stress Biology in Arid Areas, Colledge of Life Science, Northwest A&F University, Yangling, Shaanxi Province 712100, China

Received:2011-08-13 Revised:2011-10-20 Online:2012-02-15 Published:2012-07-06

扁穗冰草脱水素基因的克隆和表达特性分析

张楠, 张林生, 邢媛, 刘兰, 李嘉宏

西北农林科技大学生命科学学院旱区作物逆境生物学国家重点实验室, 陕西杨陵712100

作者简介:张楠(1986- ),男,陕西杨凌人,硕士,主要从事分子生物学研究,E-mail:yptong1790@126.com
基金资助:
国家自然科学基金项目(30671253)(31071349)资助

Abstract

Abstract: Agropyron cristatum(L.) Gaertn grows in arid,semi-arid and alpine regions possessing a strong capacity of cold and drought and disease resistance.Fragments of 3 different dehydrin genesAcwcor410,Acwzy2 and Acwcs120) and a β-actin gene(Acβ-actin) were obtained from Agropyron cristatum(L.) Gaertn leaf with RT-PCR.Semi-quantitative RT-PCR results showed that Acwcor410 was sensitive to cold stress,but insensitive to drought;Acwzy2 was sensitive to drought but not to cold;Acwcs120 had response to both cold and drought stress.Western blot results showed that there was a dehydrin of 40 kD in Agropyron cristatum(L.) Gaertn leaf.The protein varied expression with stress degree change under both cold and drought conditions.It was deduced that this protein is a drought sensitive dehydrin due to its more sensitive to drought than to cold stress.

Key words: Agropyron cristatum(L.) Gaertn, Dehydrin, Semi-quantitative RT-PCR, Western blot

摘要： 扁穗冰草(Agropyron cristatum(L.)Gaertn)生长于干旱、半干旱地区,具有耐旱、耐寒、抗病等特性。采用RT-PCR技术从扁穗冰草叶片中克隆3个脱水素基因Acwcor410,Acwzy2和Acwcs120)和1个肌动蛋白β-actin基因(Acβ-actin)。半定量RT-PCR分析表明,Acwcor410基因对温度敏感,对干旱胁迫不敏感;Acwzy2基因对温度不敏感,而对干旱胁迫敏感;Acwcs120基因则对冷胁迫及干旱胁迫均有响应。Western blot表明,扁穗冰草含有40kD脱水素,该蛋白的表达随冷胁迫和干旱胁迫程度的变化而变化,对干旱胁迫的响应比冷胁迫更为明显,推测该脱水素属于干旱敏感型。

关键词: 扁穗冰草, 脱水素, 半定量RT-PCR, 蛋白质印迹

CLC Number:

ZHANG Nan, ZHANG Lin-sheng, XING Yuan, LIU Lan, LI Jia-hong. Cloning and Expression Analysis of Dehydrin Gene from Agropyron cristatum(L.) Gaertn[J]. , 2012, 20(1): 139-145.

张楠, 张林生, 邢媛, 刘兰, 李嘉宏. 扁穗冰草脱水素基因的克隆和表达特性分析[J]. , 2012, 20(1): 139-145.

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Cloning and Expression Analysis of Dehydrin Gene from Agropyron cristatum(L.) Gaertn

扁穗冰草脱水素基因的克隆和表达特性分析

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