Genetic Diversity of Stipa bungeana Trin. Populations in Shaanxi Analyzed by ISSR Markers

doi:10.11733/j.issn.1007-0435.2012.03.020

Acta Agrestia Sinica ›› 2012, Vol. 20 ›› Issue (3): 512-517.DOI: 10.11733/j.issn.1007-0435.2012.03.020

Previous Articles Next Articles

Genetic Diversity of Stipa bungeana Trin. Populations in Shaanxi Analyzed by ISSR Markers

YU Jing¹, JING Zhao-bin², CHENG Ji-min^1,2,3

1. College of Resources and Environment, Northwest A&F University, Yangling, Shaanxi Province 712100, China;
2. College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi Province 712100, China;
3. Institute of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water Resources, Yangling, Shaanxi Province 712100, China

Received:2011-11-10 Revised:2012-01-28 Online:2012-06-15 Published:2012-07-05

陕西省本氏针茅自然种群遗传多样性的ISSR分析

俞靓¹, 井赵斌², 程积民^1,2,3

1. 西北农林科技大学资源环境学院, 陕西杨凌 712100;
2. 西北农林科技大学动物科技学院, 陕西杨凌 712100;
3. 中国科学院水利部水土保持研究所, 陕西杨凌 712100

通讯作者: 程积民,E-mail:gyzcjm@ms.iswc.ac.cn
作者简介:俞靓(1982- ),女,宁夏银川人,博士研究生,研究方向为植被生态学,E-mail:yujing82-2008@163.com
基金资助:
国家现代农业产业技术体系建设-咸阳牧草试验站;中国科学院院地合作专项"宁夏南部山区生态保育型集约化养殖技术模式转化与示范"资助

Abstract

Abstract: Inter-simple sequence repeat (ISSR) markers were used to determine the genetic diversity and genetic differentiation of five Stipa bungeana natural populations in Shaanxi province. Objectives of this study were to explore the molecular and ecology mechanisms of genetic diversity of Stipa bungeana. Fifteen primers screened from 96 primers amplified stable bands. Among a total of 223 amplified bands, 182 (81.61%) bands were polymorphic loci. Nei’s gene diversity index and Shannon’s information index were 0.1887 and 0.2975, respectively, which indicated high genetic diversity existing among population. Analysis of molecular variance (AMOVA) demonstrated genetic variation mainly among populations. Genetic variation rate was 63.01% among populations, but 36.99% within population. Moreover, the level of gene flow (N_m) was measured to be 0.1139 individual per generation among populations that indicated Stipa bungeana had a higher genetic differentiation.

Key words: Stipa bungeana Trin., ISSR, Genetic diversity, Genetic structure

摘要： 利用ISSR分子标记,对采自陕西省不同地区的5个本氏针茅(Stipa bungeana)自然种群进行遗传变异及群体遗传结构分析,旨在探讨本氏针茅遗传变异产生的分子生态机理,为其资源保护提供理论依据。在100个供试单株中,采用15条ISSR引物共扩增出223条可统计条带,其中多态性带182条,多态性位点比率为81.61%,Nei’s基因多样性(H)为0.1887,Shannon 信息指数(I)为0.2975,各种群之间存在较高的多态性。种群间的遗传变异为63.01%,种群内的遗传变异为36.99%,遗传分化系数φ_st 为0.6300,种群间的基因流(N_m)为0.1468,表明本氏针茅遗传分化程度较高,遗传变异主要分布在种群间,而种群内变异相对较小,且各种群间的基因交流非常有限。

关键词: 本氏针茅, ISSR, 遗传多样性, 遗传结构

CLC Number:

Q943

YU Jing, JING Zhao-bin, CHENG Ji-min. Genetic Diversity of Stipa bungeana Trin. Populations in Shaanxi Analyzed by ISSR Markers[J]. Acta Agrestia Sinica, 2012, 20(3): 512-517.

俞靓, 井赵斌, 程积民. 陕西省本氏针茅自然种群遗传多样性的ISSR分析[J]. 草地学报, 2012, 20(3): 512-517.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2012.03.020

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2012/V20/I3/512

References

[1] 赵念席,高玉葆,王金龙,等.大针茅种群RAPD多样性及其与若干生态因子的相关关系[J].生态学报,2006,26(5):1312-1319
[2] 卢胜莲,吴珍兰.中国针茅属植物的地理分布[J].植物分类学报,1996,34(3):242-253
[3] 周秋平,程积民,万惠娥,等.干旱胁迫下本氏针茅光合特性水分利用效率日动态研究[J].草地学报,2009,17(4):510-514
[4] 王静,程积民,万惠娥.黄土高原本氏针茅光合特性及生产力的研究[J].中国生态农业学报,2005,13(4):71-73
[5] 胡相明,程积民.施水对本氏针茅光合作用和叶绿素荧光特征的影响[J].中国水土保持科学,2006,4(2):39-46
[6] 韩凤朋,董丽娜,罗文林,等.黄土高原侵蚀区长芒草对坡地土壤水分养分的影响[J].草地学报,2008,16(4):403-407
[7] 周梦华,程积民,万惠娥,等.云雾山本氏针茅群落根系分布特征[J].草地学报,2008,16(3):267-271
[8] 周秋平,程积民,万惠娥.本氏针茅与柳枝樱光合生理生态特征比较研究[J].水土保持通报,2009,29(2):129-133
[9] 胡雪华,何亚丽,安渊,等.上海结缕草JD-1和结缕草属几个主要坪用草种的ISSR指纹分析[J].上海交通大学学报,2005,23(2):163-167
[10] 李永祥,李斯深,李立会,等.披碱草属12个物种遗传多样性的ISSR和SSR比较分析[J].中国农业科学,2005,38(8):1522-1527
[11] 魏臻武,符昕,耿小丽,等.苜蓿遗传多样性和亲缘关系的SSR和ISSR分析[J].草地学报,2007,15(2):118-123
[12] 赵桂琴,刘欢,刘美. ISSR标记的早熟禾遗传多样性分析[J].草地学报,2011,19(5):781-786
[13] 范彦,曾兵,张新全,等.中国野生鸭茅遗传多样性的ISSR研究[J].草业学报,2006,15(5):103-108
[14] 田青松,韩冰,杨劼,等. 96份雀麦属材料遗传多样性的ISSR分析[J].中国草地学报,2010,32(1):18-25
[15] Yeh F C, Boyle T J B. Population genetic analysis of codominant and dominant markers and quantitative traits [J]. Belgian Journal of Botany,1997,129(1):157
[16] 张富民,葛颂.群体遗传学研究中的数据处理方法 I. RAPD数据的AMOVA分析[J].生物多样性,2002,10(4):438-444
[17] Excofier L, Smouse P E, Quattro J M. Analysis of molecular variance inferred from metric distances among DNA haplotypes: applications to human mitochondrial DNA restriction data [J].Genetics,1992,131(2):479-491
[18] Mantel N A. The detection of disease clustering and a generalized regression approach [J].Cancer Research,1967,27(2):209-220
[19] 王东娜,牟长城,高卓,等.胡桃楸天然种群遗传多样性的ISSR分析[J].经济林研究,2011,29(2):22-29
[20] Nybom H. Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants [J]. Molecular Ecology,2004,13(5):1143-1155
[21] 鄢家俊,白史且,张新全,等.青藏高原东南缘老芒麦自然种群遗传多样性的SRAP和SSR分析[J].草业学报,2010,19(4):122-134
[22] Sun M. Effects of population size,mating system,and evolutionary origin on genetic diversity in Spiranthes sinensis and S.hongkongensis[J].Conservation Biology,1996,10(5):785-795
[23] Hamrick J L, Loveless M D. Associations between the breeding system and the genetic structure of tropical tree populations //Bock J, Linhart Y B, eds. Evolutionary ecology of plants. Westview Press, Boulder,1989:129-146
[24] Hamrick J L, Godt M J W. Allozyme diversity in plant species //Brown A H D, Clegg M T, Kahler A L, et al, eds. Plant population genetics, breeding and genetic resources. Sinauer, Sunderland, Mass,1990:43-63
[25] Wright S. The genetical structure of populations [J]. Annals of Eugenices,1951,15(1):323-354
[26] 冯亮亮,唐红,李毅,等.甘肃红砂不同种群遗传多样性的ISSR分析[J].草业学报,2011,20(1):125-130

Genetic Diversity of Stipa bungeana Trin. Populations in Shaanxi Analyzed by ISSR Markers

陕西省本氏针茅自然种群遗传多样性的ISSR分析

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	MA Cong-yu, HAN Chong-yang, MA Sai-nan, LI Xu-xu, CAI Jia-bang, WANG Yang, ZHANG Xin-quan, NIE Gang. The Fingerprints of 13 White Clover (Trifolium repens L.) Cultivars Based on SSR Markers [J]. Acta Agrestia Sinica, 2021, 29(9): 1892-1899.
[2]	LUO Ming-xin, LIU Feng-min, CHEN Ji-yan, CUI Jun-tao, ZHANG Wei-li. Agronomic Traits and Genetic Diversity Analysis of M₃ Generation of Stylosanthes spp. Irradiated by ⁶⁰Coγ [J]. Acta Agrestia Sinica, 2021, 29(9): 1992-2000.
[3]	LIANG Xiao-yu, JI Yang, HU Yuan-bin, YI Jun, BAI Shi-qie, ZHANG Jing, ZHANG Xin-quan. Exploitation and Universal Analysis of EST-SSR Molecular Markers in Cichorium intybus [J]. Acta Agrestia Sinica, 2021, 29(9): 2081-2090.
[4]	LIU Ling-yun, FAN Xi-feng, TENG Ke, YUE Yue-sen, CHANG Zhi-hui, WU Ju-ying. Advances in Development of Genetic Diversity of Carex L. [J]. Acta Agrestia Sinica, 2021, 29(6): 1148-1157.
[5]	CHEN Zhi-xiang, LUO Xiao-yan, LI Shuan-lin, WU Ru-yue, WANG Wen-qiang, DING Xi-peng. Genetic Diversity and Population Structure Analysis of the Pigeonpea (Cajanus cajanL.) Germplasm Resources Based on SSR Markers [J]. Acta Agrestia Sinica, 2021, 29(5): 904-911.
[6]	LIANG Guo-ling, LIU Wen-hui, MA Xiang. Phenotypic Diversity of the Panicle Among 590 Covered Oats Germplasm Resources in Apline of Qinghai-Tibet Plateau [J]. Acta Agrestia Sinica, 2021, 29(3): 495-503.
[7]	ZHANG Qi, WEI Zhen-wu, YAN Tian-fang, GENG Xiao-li. Identification and Evaluation of Genetic Diversity of Agronomic Traits in Oat Germplasm Resources [J]. Acta Agrestia Sinica, 2021, 29(2): 309-316.
[8]	AN Ming-zhu, HAN Bo, JIANG Hua, DUAN Xin-hui, WEN Yi-fu, ZHOU Kai. Research Progress of Dactylis Species Origin and Phylogeny Analysis [J]. Acta Agrestia Sinica, 2021, 29(12): 2637-2644.
[9]	HUANG Chun-qiong, ZHANG Zi-xiong, WANG Wen-qiang, LIU Guo-dao. Studies of SCAR Markers Linked to Pollen Sterility Gene in Stylosanthes guianensis [J]. Acta Agrestia Sinica, 2020, 28(5): 1478-1483.
[10]	WANG Jian-li, LIU Jie-lin, ZHU Rui-fen, ZHONG Peng, YOU Jia, DI Gui-li, HAN Wei-bo, SHEN Zhong-bao. Genetic Diversity Analysis of Major Agronomic Traits in 28 Amaranthus hypochondriacus Germplasm Resources [J]. Acta Agrestia Sinica, 2020, 28(4): 1050-1059.
[11]	YANG Yan-ting, SHI Feng-ling, XU Bo, ZHANG Yu-tong, YAN Wei. Identification and Genetic Diversity of Alfalfa Varieties [J]. Acta Agrestia Sinica, 2020, 28(4): 1060-1067.
[12]	MA Dong-mei, ZHANG Xi-bin, HUANG Ting, WANG Weng-jing, ZHAO Li-juan, MA Qiao-li. Genetic Diversity Analysis of 10 Salt Tolerant Alfalfa [J]. Acta Agrestia Sinica, 2019, 27(6): 1477-1485.
[13]	LIU Ya-ling, GENG Ya-ping, XIE Xiao-dong, WANG Fang, ZHANG Peng-fei. Genetic Diversity and Genetic Structure Analysis of Astragalus Based on SSR Molecular Marker [J]. Acta Agrestia Sinica, 2019, 27(5): 1154-1162.
[14]	DENG Zhen-Shan, LI Mai-ping, HAO Lei, CHEN Kai-kai, LI Jing, LIU Yu-zhen, ZHANG Bao-bao, QI Xiang-ying. Diversity and Plant Growth Promoting Activities of the Culturable Endophytic Bacteria Associated with Pennisetum sp. [J]. Acta Agrestia Sinica, 2019, 27(5): 1213-1221.
[15]	LIU Jing, ZHAO Fang-yuan, LI Dong-mei, LI Xue, TIAN Xin-hui, DU Wen-hua. Constructions on the Linkage Genetic Map and Initial Locations on QTLs Related to the Hay Yield of Forage Triticale [J]. Acta Agrestia Sinica, 2019, 27(1): 219-226.