Determination of the Capacity to Antagonistic towards Pathogenic Fungi and Identification of Anaphalis lactea Endophytic Bacteria RC14

doi:10.11733/j.issn.1007-0435.2012.04.025

›› 2012, Vol. 20 ›› Issue (4): 753-758.DOI: 10.11733/j.issn.1007-0435.2012.04.025

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Determination of the Capacity to Antagonistic towards Pathogenic Fungi and Identification of Anaphalis lactea Endophytic Bacteria RC14

LI Zhen-dong^1,2, CHEN Xiu-rong¹, YANG Cheng-de¹, LI Peng¹

1. Pratacultural College, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem Ministry of Education, Grass Engineering Laboratory of Gansu Province, Sino-U.S. Centers for Grazingland Ecosystem Sustainability, Lanzhou, Gansu Province 730070, China;
2. Institute of Rural Development, Gansu Academy of Social Sciences, Lanzhou, Gansu Province 730070, China

Received:2011-12-19 Revised:2012-02-20 Online:2012-08-15 Published:2012-08-28

乳白香青内生菌RC14抑菌能力测定及其鉴定

李振东^1,2, 陈秀蓉¹, 杨成德¹, 李鹏¹

1. 甘肃农业大学草业学院草业生态系统教育部重点实验室甘肃省草业工程实验室中-美草地畜牧业可持续发展研究中心, 甘肃兰州 730070;
2. 甘肃省社会科学院农村发展研究所, 甘肃兰州 730070

通讯作者: 陈秀蓉
作者简介:李振东(1978-),男,甘肃临夏人,副研究员,博士,研究方向为草地生物多样性,E-mail:lizhendong22@163.com
基金资助:
国家自然基金项目(31160122);草业生态系统教育部重点实验室(甘肃农业大学)开放课题(CYzs-2011011)资助

Abstract

Abstract: The study is focused on the endophytic bacterium RC14 of dominant plant Anaphalis lactea in alpine grassland. Antimicrobial spectra of RC14 are determined using PDA-plate confrontation. Morphological, physiological and biochemical characteristics of RC14 are identified using 16S rDNA gene sequence analysis. Results show that RC14 has a capacity to inhibit roots pathogens and leaf spot pathogens in several varieties of plants, and can inhibit pathogenic fungi such as Bipolaris, Botrytis, Fusarium, Alternaria and Rhizoctonia indicating a wide antimicrobial spectrum of RC14. RC14 is gram-positive with 1.9~3.3 μm×0.7~0.8 μm rod-shape and can produce spore in the center. Morphological characteristics, physiological and biochemical characteristics and 16S rDNA gene sequence analysis show that RC14 is Bacillus subtilis. This study will provide evidence for exploring and utilizing antimicrobial endophytic bacteria in alpine grassland and will also provide strain resource for making biological pesticides.

Key words: Alpine grassland, Antagonistic endophytic bacteria, Bacillus subtilis, Identification

摘要： 以高寒草地优势植物乳白香青(Anaphalis lactea)内生细菌RC14为研究对象,采用平板对峙法测定其对植物病原真菌的抑菌能力;并利用16S rDNA序列分析结合形态特征和生理生化特性对RC14进行鉴定。结果表明:RC14对多种植物根部病原菌和叶斑病菌均具有良好的抑制作用,抑制的病原菌种类有葡萄孢属(Botrytis)、蠕孢属(Bipolaris)、链格孢属(Alternaria)、镰孢菌属(Fusarium)和丝核菌属(Rhizoctonia)等植物病原真菌属,说明RC14具有较广的抑菌谱。RC14菌体杆状,大小为1.9~3.3 μm×0.7~0.8 μm, G⁺,中生芽孢,形态特征和生理生化特性及16S rDNA基因序列综合分析结果表明RC14为枯草芽孢杆菌(Bacillus subtilis)。本研究结果为开发和利用高寒草地所特有植物的内生拮抗菌提供了理论依据,为开发生物农药提供菌种资源。

关键词: 高寒草地, 内生拮抗菌, 枯草芽孢杆菌, 鉴定

CLC Number:

Q946
S432

LI Zhen-dong, CHEN Xiu-rong, YANG Cheng-de, LI Peng. Determination of the Capacity to Antagonistic towards Pathogenic Fungi and Identification of Anaphalis lactea Endophytic Bacteria RC14[J]. , 2012, 20(4): 753-758.

李振东, 陈秀蓉, 杨成德, 李鹏. 乳白香青内生菌RC14抑菌能力测定及其鉴定[J]. , 2012, 20(4): 753-758.

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Determination of the Capacity to Antagonistic towards Pathogenic Fungi and Identification of Anaphalis lactea Endophytic Bacteria RC14

乳白香青内生菌RC14抑菌能力测定及其鉴定

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