Protective Effects of Exogenous Nitric Oxide on Oxidative Damage of Maize Seedling Leaves under NaCl Stress

doi:10.11733/j.issn.1007-0435.2012.06.022

›› 2012, Vol. 20 ›› Issue (6): 1117-1122.DOI: 10.11733/j.issn.1007-0435.2012.06.022

Previous Articles Next Articles

Protective Effects of Exogenous Nitric Oxide on Oxidative Damage of Maize Seedling Leaves under NaCl Stress

WANG Fang, WANG Han-ning

Department of Agronomy, Gansu Agricultural University, Key Laboratory of Arid Land Crop Science in Gansu Province, Gansu Key Labortory of Crop Improvement and Germplasm Enhancement, Lanzhou, Gansu Province 730070, China

Received:2012-06-26 Revised:2012-10-25 Online:2012-12-15 Published:2012-12-28

外源NO对NaCl胁迫下玉米幼苗氧化损伤的保护作用

王芳, 王汉宁

甘肃农业大学农学院甘肃省干旱生境作物学重点实验室甘肃省作物遗传改良与种质创新重点实验室, 甘肃兰州 730070

通讯作者: 王汉宁
作者简介:王芳(1980-),女,甘肃民勤人,博士研究生,讲师,主要从事种子生理及玉米分子育种方面的研究,E-mail:wangfang@gsau.edu.cn;
基金资助:
甘肃农业大学盛彤笙科技创新基金(GSAU-STS-1229);973计划前期研究专项(2012CB722902);科技部成果转化项目(2010GB2G100484)资助

Abstract

Abstract: In order to clarify the physiological mechanism of nitric oxide (NO) on relief maize seedling growth, the protective effects of exogenous nitric oxide donor (sodium nitroprusside, SNP) at different concentrations on maize seedling oxidative damage due to 150 mmol稬^-1 NaCl stress were investigated. Result showed that SNP significantly increased the antioxidant activities of SOD, CAT and POD, increased the proline and chlorophyll contents of leaves, while significantly decreased the MDA contents of leaves under NaCl stress compared with control. SNP had dosage effect on alleviating the NaCl stress of maize seedlings. The SNP of 0.1 mmol稬^-1significantly elevated plant salt tolerance and had the best alleviating effect on NaCl stress damage.

Key words: Maize seedling, Nitric oxide, NaCl stress, Oxidative damage

摘要： 试验以150 mmol·L^-1 NaCl为胁迫条件,用浓度为0.01,0.05,0.1,0.5和1 mmol·L^-1的外源一氧化氮(NO)供体SNP处理玉米(Zea mays L.)幼苗,研究外源NO对NaCl胁迫下玉米幼苗氧化损伤的影响,旨在探讨NO对NaCl胁迫下玉米生长缓解的生理机理。结果表明:外源NO可促进脯氨酸(Pro)积累,提高超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性,提高可溶性糖的含量,并能缓解叶绿素的降解和丙二醛(MDA)含量的升高。NO的这种作用存在明显的剂量效应,其中以0.1 mmol·L^-1 SNP处理效果最为显著。

关键词: 玉米幼苗, 一氧化氮, NaCl胁迫, 氧化损伤

CLC Number:

Q945.78
S513

WANG Fang, WANG Han-ning. Protective Effects of Exogenous Nitric Oxide on Oxidative Damage of Maize Seedling Leaves under NaCl Stress[J]. , 2012, 20(6): 1117-1122.

王芳, 王汉宁. 外源NO对NaCl胁迫下玉米幼苗氧化损伤的保护作用[J]. , 2012, 20(6): 1117-1122.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2012.06.022

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2012/V20/I6/1117

References

[1] 王征宏,吕淑芳,张亚冰.盐胁迫下外源NO对玉米叶片氮代谢产物的影响[J].安徽农业科学,2007,35(17):5055- 5056
[2] Boyer J S. Plant productivity and environment [J]. Science,1982,218(4571):443-448
[3] 樊怀福,郭世荣,焦彦生,等.外源一氧化氮对NaCl胁迫下黄瓜幼苗生长、活性氧代谢和光合特性的影响[J].生态学报,2007,27(2):546-553
[4] 李运合,钱善勤,覃逸明.盐胁迫下外源NO对玉米幼苗叶片蔗糖代谢的影响[J].湖北农业科学,2010,49(7):1589-1592
[5] 刘友良,汪良驹.植物对盐胁迫的反应和耐盐性[C]//余叔文,汤章城,编.植物生理与分子生物学.北京:科学技术出版社,1998:752-769
[6] 刘建新,胡浩斌,王鑫.外源NO对盐胁迫下黑麦草幼苗活性氧代谢、多胺含量和光合作用的影响[J].植物研究,2009,29(3): 313-319
[7] Neill S J, Desikan R, Hancock J T. Nitric oxide signaling in plants[J]. New Phytologist,2003,159(1):11-35
[8] Zhang H, Shen W B, Xu L L. Effect of nitric oxide on the germination of wheat seed and its reactive oxygen species metabolisms under osmotic stress[J]. Acta Botanica Sinica,2003,45(8):901-905
[9] 阮海华,沈文飚,徐朗莱. 一氧化氮调节盐胁迫下小麦幼苗根部质膜H⁺-ATPase和焦磷酸酶活性提高耐盐性[J].植物学报,2004,46(4):415-422
[10] 阮海华,沈文飚,叶茂炳,等. 一氧化氮对盐胁迫下小麦叶片氧化损伤的保护效应[J].科学通报,2001,46(23):1993-1997
[11] Uchida A, Jagendorf A T, Hibino T, et al. Effects of hydrogen peroxide and nitric oxide on both salt and heat stress tolerance in rice[J]. Plant Science,2002,163(3):515-523
[12] 田曾元,王松,张虹,等. 外源NO提高海滨锦葵耐盐性[J].东北林业大学学报,2009,37(7):62-64
[13] 吴雪霞,朱月林,朱为民,等.外源一氧化氮对NaCl胁迫下番茄幼苗生长和光合作用的影响[J].西北植物学报,2006,26(6):1206-1211
[14] 樊怀福,郭世荣,焦彦生,等.外源一氧化氮对NaCl胁迫下黄瓜幼苗生长、活性氧代谢和光合特性的影响[J].生态学报,2007,27(2):546-553
[15] 张艳艳,刘俊,刘友良.一氧化氮缓解盐胁迫对玉米生长的抑制作用[J].植物生理与分子生物学学报,2004,30(4):455-459
[16] Chander M S. Enzymic associations with resistance to rust and powdery mildew in pea [J].Indian Journal of Horticulture,1990,47(3):341-345
[17] 张志良.植物生理学实验指导[M].北京:高等教育出版社,2009:218-219
[18] 陈建勋,王晓峰.植物生理学实验指导[M].广州:华南理工大学出版社,2002:68-72
[19] 李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社, 2000:258-260
[20] 邹琦.植物生理生化实验指导[M].北京:中国农业出版社,1995:33-36
[21] 张绪成,上官周平,高世铭. NO 对植物生长发育的调控机制[J].西北植物学报,2005,25(4):812-818
[22] 刘建新,胡浩斌,王鑫.外源NO对盐胁迫下黑麦草幼苗根生长抑制和氧化损伤的缓解效应[J].植物研究,2008,28(1):7-13
[23] 阮海华,沈文飚,叶茂炳,等.一氧化氮对盐胁迫下小麦叶片氧化损伤的保护效应[J].科学通报,2001,46(23):1993-1997
[24] Zhao L Q, Zhang F, Guo J K, et al. Nitric oxide functions as a signal in salt resistance in the calluses from two ecotypes of reed[J]. Plant Physiology,2004,134(2):849-857
[25] Shi Q H, Ding F, Wang X F, et al. Exogenous nitric oxide protect cucumber roots against oxidative stress induced by salt stress[J]. Plant Physiology and Biochemistry,2007,45(8):542-550
[26] 路莹,王萍,宋兆伟.外源NO浸种对NaCl胁迫下沙葱种子萌发和生理特性的影响[J].华北农学报,2011,26(2):207-212
[27] 吴雪霞,朱月林,朱为民.外源一氧化氮对 NaCl 胁迫下番茄幼苗生理影响[J].中国农业科学,2006,39(3):575-581
[28] 李明,王根轩.干旱胁迫对甘草幼苗保护酶活性及脂质过氧化作用的影响[J].生态学报,2002,22(4):503-507
[29] Mata C G, Lamatina L. Nitric oxide induces stomata closure and enhances the adaptive plant responses against drought stress[J].Plant Physiology,2001,126(3):1196-1204
[30] 马秀芳,沈秀瑛.不同耐旱性玉米品种对干旱的生理生化反应[J].沈阳农业大学学报,2002,33(3):167-170
[31] 郝岗平,杜希华,史仁玖.干旱胁迫下外源一氧化氮促进银杏可溶性糖、脯氨酸和次生代谢产物合成[J].植物生理与分子生物学学报,2007,33(6):499-506
[32] 马向丽,魏小红,龙瑞军,等.外源一氧化氮提高一年生黑麦草抗冷机制[J].生态学报,2005,25(6):1269-1274
[33] 陈明,沈文飚,阮海华,等.一氧化氮对盐胁迫下小麦幼苗根生长和氧化损伤的影响[J].植物生理与分子生物学报,2004,30(5):569-576
[34] 杜菲,陈新,杨春华.NaCl胁迫对不同柳枝稷材料种子萌发与幼苗生长的影响[J].草地学报,2011,19(6):1018-1024
[35] Munns R. Physiological processes limiting plant growth in saline soils-some dogmas and hypotheses [J]. Plant, Cell and Environment,1993,16(1):15-24
[36] 孙振雷,刘鹏,叶柏军.绿豆种子萌发及苗期抗盐性的研究[J].内蒙古民族大学学报,2001,16(10):31-38
[37] 蒋明义,杨文英,徐江,等.渗透胁迫下水稻幼苗中叶绿素降解的活性氧损伤作用[J].植物学报,1994,36(4):289-295

Protective Effects of Exogenous Nitric Oxide on Oxidative Damage of Maize Seedling Leaves under NaCl Stress

外源NO对NaCl胁迫下玉米幼苗氧化损伤的保护作用

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Wen-jing, MA Dong-mei, ZHAO Li-juan, MA Qiao-li. Effects of 2,4-table Brassinolide on Enzyme Activity and Root ion Distribution and Absorption in Alfalfa Seedlings [J]. Acta Agrestia Sinica, 2021, 29(6): 1363-1368.
[2]	LI Tian-yong, YAN Zi-zhu, JIANG Sheng-xiu. Responses of Seed Germination of Two Lepidium Species to Different Concentrations of NaCl Stress [J]. Acta Agrestia Sinica, 2021, 29(1): 88-94.
[3]	FU Jing-yan, LIANG Lin-lin, ZHOU Min, LI Zhou. Effects of seeds soaking with γ-aminobutyric acid on germination and aluminium tolerance of white clover [J]. Acta Agrestia Sinica, 2020, 28(5): 1275-1284.
[4]	LUO Xiao-yan, LI Xin-yong, ZHANG Yu, WANG Wen-qiang, DING Xi-peng. Difference Analysis of Salt and Drought Tolerance of Eight Desmodium Species Seeds During Germination [J]. Acta Agrestia Sinica, 2020, 28(5): 1329-1336.
[5]	KOU Jiang-tao. Effects of 2,4-Epibrassinolide on Germination and Physiological Characteristics of Avena sativa L. Seeds under NaCl Stress [J]. Acta Agrestia Sinica, 2019, 27(6): 1562-1568.
[6]	LI Bo, FANG Zhi-jian, LI Hong, YANG Zhao. Effects of ⁶⁰Co-γ Radiation on Resistance of Medicago sativa×Meliloides ruthenica ‘Longmu 806’ Seedlings to NaCl Stress [J]. Acta Agrestia Sinica, 2019, 27(5): 1259-1265.
[7]	JIA Xiang-yang, CHONG Pei-fang, ZHANG Yu-jie, LI Yi, SU Shi-ping. Effects of Nitric Oxide on Physiological Characteristics and Growth of Reaumuria soongorica Seedling under NaCl Stress [J]. Acta Agrestia Sinica, 2019, 27(3): 628-636.
[8]	MA Yan-fang, ZHANG Xu-hu, MENG Wen-wen, CHAI Rui, QI Yan-wen, XU Yue-fei. Effects of Nitric Oxide and Mowing Heights on Root Morphological Characteristics of Tall Fescue [J]. Acta Agrestia Sinica, 2017, 25(4): 777-781.
[9]	TIAN Xiao-xia, MAO Pei-chun, LI Shan-shan, GUO Qiang, SUN Jian-ming, ZHANG Lin, MENG Lin. Determination of Salt-tolerant Indexes and Comprehensive Evaluation of Salt-tolerance at the Seedlings of Alfalfa [J]. Acta Agrestia Sinica, 2017, 25(3): 545-553.
[10]	CAI Dan-hong, LI Chen-jian, WEI Yan. Germination Behavior of Ceratoides latens Seed in Junggar Desert [J]. Acta Agrestia Sinica, 2016, 24(2): 384-388.
[11]	LIU Ai-rong, ZHANG Yuan-bing, ZHANG Xue-ping, YE Mei-rong, ZHAN Qiu-wen. Responses of Growth and Physiology Metabolism of Ophiopogon japonicus to NaCl Stress [J]. Acta Agrestia Sinica, 2016, 24(1): 93-100.
[12]	ZHANG Kai-hui, GAO Su-ping, WEN Jin-yan, LEI Ting, LIN Xiao. Ameliorating Effects of Exogenous Nitric Oxide on Festuca arundinacea under Cadmium Stress [J]. Acta Agrestia Sinica, 2015, 23(2): 338-345.
[13]	FU Juan-juan, SUN Yong-fang, CHU Xi-tong, XU Yue-fei, HU Tian-ming. Physiological Regulation of Nitric Oxide (NO) to the Antioxidant System of Tall Fescue Leaves under Shade Stress [J]. , 2014, 22(1): 107-115.
[14]	WANG Wen, JIANG Wen-lan, XIE Zhong-kui, ZHANG De-gang, KOU Jiang-tao. Effect of NaCl Stress on Physiological Index of Nitraria tangutorum Seedling [J]. , 2012, 20(5): 907-913.
[15]	XIONG Xue, ZHOU He, GAO Wei-ping. Physiological Regulation of Exogenous Nitric Oxide to Tall Fescue under Shade Stress [J]. , 2012, 20(2): 331-335.