Dynamic Responses of Antioxidative Defense System of Lespedeza davurica to Drought and Enhanced UV-B Radiation

doi:10.11733/j.issn.1007-0435.2013.02.015

›› 2013, Vol. 21 ›› Issue (2): 308-315.DOI: 10.11733/j.issn.1007-0435.2013.02.015

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Dynamic Responses of Antioxidative Defense System of Lespedeza davurica to Drought and Enhanced UV-B Radiation

ZHAO Jie, DU Run-feng, WANG Long-fei, WANG Yu-ying, HAO Wen-fang

Department of Life Science, Northwest A&F University, Yangling, Shaanxi Pronvince 712100, China

Received:2012-09-27 Revised:2012-12-13 Online:2013-04-15 Published:2013-04-23

达乌里胡枝子抗氧化防御系统对干旱及增强UV-B辐射的动态响应

赵洁, 杜润峰, 王龙飞, 王钰莹, 郝文芳

西北农林科技大学生命科学学院, 陕西杨凌 712100

通讯作者: 郝文芳,haowenfang2002@tom.com
作者简介:赵洁(1988-),女,陕西子长人,硕士研究生,研究方向为植物逆境生理,E-mail:zhao19880515jie@163.com
基金资助:
林业公益性行业科研专项(201304312);中国科学院"西部之光"人才培养项目(2008DF02)资助

Abstract

Abstract: The effects of drought and enhanced UV-B radiation on the antioxidant defense system and membrane lipid peroxidation in Lespedeza davurica were investigated. Compared with the single-stress of drought, the co-stresses of drought and enhanced UV-B radiation decreased the activities of superoxide dismutase (SOD) and catalase (CAT), and increased the activities of ascorbate peroxidase (APX) and peroxidase (POD). SOD activity under the co-stresses of drought and low UV-B radiation intensity was significantly lower than that under the single-stress of drought. With the increase of UV-B radiation intensity under co-stresses, POD activity decreased while activities of APX and CAT increased. Compared with the single-stress of drought, the co-stresses of drought and different UV-B radiation intensities increased ascorbic acid (AsA) content and decreased the contents of chlorophyll (Chl) and carotenoid (Car). With the increase of UV-B intensity under co-stresses, AsA content initially increased then decreased while the contents of Chl and Car decreased. During the first six days of the co-stresses of drought and low UV-B radiation intensity, anthocyanin content significantly increased compared with control. During the early stage of the co-stresses of drought and enhanced UV-B radiation, the contents of malonaldehyde (MDA) and O₂^- decreased compared with the single-stress of drought. Comprehensive evaluation according to subordinate function indicated that both single-stress of drought and co-stresses could increase the antioxidant ability of Lespedeza davurica, and the co-stresses of drought and low UV-B intensity improved antioxidant ability more.

Key words: Drought stress, Enhanced UV-B radiation, Co-stresses, Lespedeza davurica, Antioxidant defense system

摘要： 以达乌里胡枝子(Lespedeza davurica)为研究对象,通过对照、中度干旱以及干旱与增强UV-B辐射复合处理,对其超氧阴离子(O₂^-)、丙二醛(MDA)和抗氧化剂含量及抗氧化酶活性的动态变化进行研究,探讨其抗氧化能力及膜脂过氧化的动态响应。结果表明:与单因子干旱胁迫相比,复合胁迫使超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性降低,抗坏血酸过氧化物酶(APX)及过氧化物酶(POD)活性升高,其中低强度UV-B辐射与干旱复合胁迫使 SOD活性极显著低于单因子干旱胁迫下的(P<0.01),POD活性随UV-B辐射强度增加而降低,APX和CAT活性随UV-B辐射强度增加而增加。与单因子干旱胁迫相比,复合胁迫使抗坏血酸(AsA)含量增加,且随UV-B辐射强度的增加先增加后降低;使叶绿素(Chl)和类胡萝卜素(Car)含量降低,随UV-B辐射强度增加而降低。低强度UV-B辐射与干旱复合胁迫前6 d花青素含量极显著高于对照(P<0.01)。复合胁迫前期,MDA和O₂^-含量均比单因子干旱胁迫的低,后期则不同。隶属函数综合评价得出,中度干旱以及复合胁迫均能提高达乌里胡枝子的抗氧化能力,且与干旱胁迫相比,低强度UV-B辐射与干旱复合胁迫下其抗氧化能力较高,而高强度UV-B辐射与干旱复合胁迫下则较低。

关键词: 干旱胁迫, 增强UV-B辐射, 复合胁迫, 达乌里胡枝子, 抗氧化防御系统

CLC Number:

Q945.79

ZHAO Jie, DU Run-feng, WANG Long-fei, WANG Yu-ying, HAO Wen-fang. Dynamic Responses of Antioxidative Defense System of Lespedeza davurica to Drought and Enhanced UV-B Radiation[J]. , 2013, 21(2): 308-315.

赵洁, 杜润峰, 王龙飞, 王钰莹, 郝文芳. 达乌里胡枝子抗氧化防御系统对干旱及增强UV-B辐射的动态响应[J]. , 2013, 21(2): 308-315.

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Dynamic Responses of Antioxidative Defense System of Lespedeza davurica to Drought and Enhanced UV-B Radiation

达乌里胡枝子抗氧化防御系统对干旱及增强UV-B辐射的动态响应

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