Synergistic Effects of Doubled CO2 Concentration and Drought Stress on the Photosynthetic Characteristics of Medicago sativa

doi:10.11733/j.issn.1007-0435.2014.01.014

›› 2014, Vol. 22 ›› Issue (1): 85-93.DOI: 10.11733/j.issn.1007-0435.2014.01.014

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Synergistic Effects of Doubled CO₂ Concentration and Drought Stress on the Photosynthetic Characteristics of Medicago sativa

FAN Liang-xin^1,2, LIU Guo-bin^1,3, XUE Sha^1,3, YANG Ting¹, ZHANG Chang-sheng¹

1. College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi Province 712100, China;
2. School of Surveying and Land Information Engineering, Henan Polytechnic University, Jiaozuo, Henan Province 454003, China;
3. Institute of Soil and Water Conservation, Chinese Academy of Science and Ministry of Water Resources, Yangling, Shaanxi Province 712100, China

Received:2013-05-16 Revised:2013-07-15 Online:2014-02-15 Published:2014-01-28

CO₂浓度倍增及干旱胁迫对紫花苜蓿光合生理特性的协同影响

樊良新^1,2, 刘国彬^1,3, 薛萐^1,3, 杨婷¹, 张昌胜¹

1. 西北农林科技大学资源环境学院, 陕西杨凌 712100;
2. 河南理工大学测绘与国土信息工程学院, 河南焦作 454003;
3. 中国科学院水利部水土保持研究所, 陕西杨凌 712100

通讯作者: 薛萐，E-mail：xuesha100@163.com
作者简介:樊良新（1979-），男，安徽六安人，博士研究生，主要从事生态规划管理研究，E-mail：fanliangxin@163.com
基金资助:
西北农林科技大学基本科研业务费专项（ZD2013021）；中国科学院战略性先导科技专项（XDA05060300）；陕西省科学技术研究发展计划项目（2011KJXX63）资助

Abstract

Abstract: In order to study the photo-physiological characteristics of Medicago sativa in the loess hilly-gully region under different water stress and CO₂ concentration, the photosynthetic and physiological characteristics of plant were analyzed with different scenarios using artificial climate chamber and water control simulation. Results showed that the photo-physiological characteristics of alfalfa were significantly affected by drought stress and doubled CO₂ concentration, and there were synergistic effects between them. Under drought stress, the doubled CO₂ concentration improved G_s, T_r and LSP, but had no effect on P_max, C_i and AQE, as compared with those under ambient CO₂ concentration. The water use efficiency (WUE) under double CO₂ concentration was higher than under normal CO₂ concentration, but had no significant correlation with moisture content. Drought stress and doubled CO₂ concentration had significantly effects on photosynthetic pigment, malondialdehyde (MDA) and proline (Pro). Drought stress, to a certain extent, decreased the chlorophyll a (chl a), chlorophyll b (chl b) and the total chlorophyll contents, while increased significantly the MDA and Pro contents of plant. Doubled CO₂ concentration decreased the photosynthetic pigment contents of plant, and improved the MDA and Pro contents of plant. However, there were no significant synergistic effects between them. Drought stress and double CO₂ concentration had different effects on the fluorescence parameters of chlorophyll, and there was a strong synergistic effect between them. Under drought stress, doubled CO₂ concentration had more obvious protection on the photosynthetic electron transport in the plants. Therefore, the above results indicated that in arid and semi-arid area, doubled CO₂ concentration had definite compensation effects on the photosynthetic physiological functions of alfalfa induced by drought stress, and enhanced drought resistance capacity, improved water use efficiency and alleviated the negative effects of drought stress on alfalfa.

Key words: Medicago sativa, Photo-physiological characteristics, Doubled CO₂ concentration, Drought stress, Synergic effect

摘要： 为探讨CO₂浓度倍增和干旱胁迫对紫花苜蓿（Medicago sativa）生理作用的协同效应，采用人工气候室和控水试验模拟CO₂浓度倍增和干旱胁迫，分析了植物光合和抗逆生理特征的变化规律。结果表明：干旱胁迫与CO₂浓度倍增对苜蓿光响应参数影响显著，二者之间具有一定的交互作用，在干旱胁迫下，CO₂浓度倍增减缓了苜蓿叶片气孔导度（G_s）、蒸滕速率（T_r）和光饱和点（LSP）的降低幅度，而对最大净光合速率（P_max）、胞间CO₂浓度（C_i）和表观量子效率（AQE）没有减缓作用。CO₂浓度倍增下的水分利用效率（WUE）高于正常CO₂浓度，而与水分含量关系不大。干旱胁迫与CO₂浓度倍增对苜蓿光合色素、丙二醛（MDA）和脯氨酸（Pro）含量影响显著，干旱胁迫在一定程度上降低了叶绿素a（chl a）、叶绿素b（chl b）的含量和叶绿素总量，显著增加了叶片MDA和Pro含量，CO₂浓度倍增有增加这些光合色素含量、降低MDA和Pro的趋势；但2个因素之间没有显著交互作用。干旱胁迫和CO₂浓度倍增对苜蓿叶绿素荧光参数影响不一，干旱胁迫和CO₂浓度倍增对叶绿素荧光参数具有较强的交互作用，CO₂浓度倍增对植物体光合电子传递过程中的保护作用在植物受到干旱胁迫时更加明显。因此，在干旱、半干旱地区，干旱胁迫对苜蓿光合生理功能产生不利的影响，而CO₂浓度倍增对苜蓿的生长具有一定的施肥效应，当2个因素共同发生时，CO₂浓度倍增可以减缓水分胁迫对苜蓿的伤害，增强其抗旱能力，提高水分利用效率，缓解干旱胁迫的负面效应。

关键词: 紫花苜蓿, 光合生理特性, CO₂浓度倍增, 干旱胁迫, 协同效应

CLC Number:

FAN Liang-xin, LIU Guo-bin, XUE Sha, YANG Ting, ZHANG Chang-sheng. Synergistic Effects of Doubled CO₂ Concentration and Drought Stress on the Photosynthetic Characteristics of Medicago sativa[J]. , 2014, 22(1): 85-93.

樊良新, 刘国彬, 薛萐, 杨婷, 张昌胜. CO₂浓度倍增及干旱胁迫对紫花苜蓿光合生理特性的协同影响[J]. , 2014, 22(1): 85-93.

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Synergistic Effects of Doubled CO₂ Concentration and Drought Stress on the Photosynthetic Characteristics of Medicago sativa

CO₂浓度倍增及干旱胁迫对紫花苜蓿光合生理特性的协同影响

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