Effects of Cu2+and Zn2+ Stress on the Bulbil Germination and Physiological-biochemical Characteristics of Polygonum viviparum

doi:10.11733/j.issn.1007-0435.2014.01.018

›› 2014, Vol. 22 ›› Issue (1): 116-121.DOI: 10.11733/j.issn.1007-0435.2014.01.018

Previous Articles Next Articles

Effects of Cu²⁺and Zn²⁺ Stress on the Bulbil Germination and Physiological-biochemical Characteristics of Polygonum viviparum

ZHAO Yu-hong, MENG Zu-qing, NIU Xin-yu, JING Jiu-wang, WEI Xue-hong

Agriculture and Animal Husbandry College, Tibet University, Linzhi, Tibet 860000, China

Received:2013-05-22 Revised:2013-07-02 Online:2014-02-15 Published:2014-01-28

铜、锌胁迫对珠芽蓼珠芽萌发及生理生化特性的影响

赵玉红, 蒙祖庆, 牛歆雨, 敬久旺, 魏学红

西藏大学农牧学院, 西藏林芝 860000

通讯作者: 敬久旺，E-mail：jjw-ok@163.com
作者简介:赵玉红（1979-），男，甘肃武威人，副教授，硕士，主要从事草地生态学的研究与教学，E-mail：yuhong19801011@126.com
基金资助:
国家自然基金（31160112）（31240019）；教育部青年基金（10XZJC850002）资助

Abstract

Abstract: In order to understand the remediation mechanism of copper-zinc mine soil by Polygonum viviparum, the effects of Cu and Zn stress on the bulbil germination rate, seedling growth and physiological-biochemical characteristics of Polygonum viviparum were investigated in this study. Results showed that the germination rate of bulbil decreased when Cu²⁺ concentration was more than 40 mg·L^-1 or Zn²⁺ more than 20 mg·L^-1. The seedling height and root length of Polygonum viviparum were correlated with the concentrations of Cu⁺²and Zn²⁺. Cu²⁺ had more significant effects on the seedling growth and root elongation of bulbil compared to Zn²⁺. The chlorophyll a and chlorophyll b contents of bulbils decreased with the increase of Cu²⁺or Zn²⁺ concentration; whereas the conductivity and malonaldehyde (MDA) contents of leaves increased. And all tested indexes were correlated with the concentrations of Cu²⁺and Zn²⁺.

Key words: Cu stress, Zn stress, Polygonum viviparum L., Bulbil germination, Physiological-biochemical characteristics

摘要： 为了探索珠芽蓼（Polygonum viviparum L.）修复铜、锌矿山土壤的机制，以珠芽蓼珠芽为材料，采用溶液培养试验研究了不同浓度的铜（Cu²⁺）和锌（Zn²⁺）胁迫对珠芽蓼珠芽萌发、生长及生理生化特性的影响。结果表明：Cu²⁺浓度达到40 mg·L^-1时，发芽率下降；Zn²⁺浓度达到20 mg·L^-1时，发芽率下降。不同浓度Cu²⁺和Zn²⁺与珠芽蓼幼苗高度和根长相关，Cu²⁺对珠芽蓼珠芽幼苗生长和根系伸长的抑制作用比Zn²⁺更显著。随着Cu²⁺和Zn²处理浓度的升高，珠芽蓼叶绿素a、叶绿素b含量降低；叶片电导率和丙二醛（MDA）含量增大，并与处理浓度呈一定的相关关系。珠芽蓼虽能够在西藏中部矿区正常生长而具有一定的耐性，但在铜、锌污染土壤的植物修复中没有较强的应用潜力。

关键词: 铜胁迫, 锌胁迫, 蓼珠芽, 珠芽萌发, 生理生化特性

CLC Number:

Q945.78

ZHAO Yu-hong, MENG Zu-qing, NIU Xin-yu, JING Jiu-wang, WEI Xue-hong. Effects of Cu²⁺and Zn²⁺ Stress on the Bulbil Germination and Physiological-biochemical Characteristics of Polygonum viviparum[J]. , 2014, 22(1): 116-121.

赵玉红, 蒙祖庆, 牛歆雨, 敬久旺, 魏学红. 铜、锌胁迫对珠芽蓼珠芽萌发及生理生化特性的影响[J]. , 2014, 22(1): 116-121.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2014.01.018

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2014/V22/I1/116

References

[1] 梁锦, 吕文超, 何俊国, 等.西藏矿产资源可持续开发综述[J].中山大学研究生学刊, 2009, 30(3):31-38
[2] 陈怀满.土壤-植物系统中的重金属污染[M].北京:科学出版社, 1996:294-295
[3] Foyer C H, Lelandais M, Kunert K J. Photo oxidative stress in plant[J].Physiologia Plantarum, 1994, 92(4):708-719
[4] 徐勤松, 施国新, 周红卫, 等.Cd、Zn复合污染对水车前叶绿素含量和活性氧清除系统的影响[J].生态学杂志, 2003, 22(1):5-8
[5] 南忠仁, 李吉均.干旱区污灌土壤作物系统Cu、Zn的行为特征盐湖研究, 2001, 9(1):25-28
[6] 冯淑利, 邵云, 刘洋, 等.Zn²⁺胁迫对小麦幼苗生理活性影响的研究[J].农业环境科学学报, 2007, 26(1):140-145
[7] 钱海燕, 王兴祥, 蒋佩兰.黑麦草连茬对铜、锌污染土壤的耐性及其修复作用[J].江西农业大学学报, 2004, 26(5):801-804
[8] 昝树婷, 杨如意, 汪伟伟.土壤理化性质与海州香薷铜锌积累的关系[J].生物学杂志, 2011, 28(1):46-49
[9] 黄五星, 高境清, 黄宇, 等.商陆对镉锌铜胁迫的生理响应与金属积累特性[J].环境科学与技术, 2010, 33(1):77-79
[10] 徐小逊, 张世熔, 徐红娇, 等.铜胁迫下垂序商陆的生长响应和铜富集特性[J].四川农业大学学报, 2012, 30(1):37-41
[11] 李有忠, 沈颂东.珠芽蓼营养及繁殖器官的形态结构研究[J].青海师范大学学报:自然科学版, 1996(1):34-41
[12] 王俊丽, 公维镇, 肖璇, 等.藏药珠芽蓼的研究与应用[J].中央民族大学学报, 2010, 19(1):28-33
[13] 赵玉红, 张涪平, 王忠红.藏中矿区植物种群生态位特征研究[J].青海草业, 2009, 18(4):1-7
[14] 敬久旺, 赵玉红, 张涪平, 等.藏中矿区表层土壤重金属污染评价[J].贵州农业科学, 2011, 39(7):126-128
[15] 李振国.植物细胞质膜透性的测定[M].北京:科学出版社, 1999:302-303
[16] 王学奎.植物生理生化实验原理和技术[M].北京:高等教育出版社, 2006:134-136, 280-281
[17] 邹园.珠芽蓼的开花结实与胎生繁殖[D].雅安:四川农业大学, 2009:48
[18] 段昌群, 王焕校.Pb²⁺、Cd²⁺、Hg²⁺对蚕豆乳酸脱氢酶的影响[J].生态学报, 1998, 18(4):413-417
[19] 钟士传.汞胁迫对花生种子发芽及生理生化特性的影响[J].种子, 2009, 28(12):89-101
[20] 吕静霞, 张雅莉.铅胁迫对小麦种子萌发及幼苗生长的影响[J].种子, 2012, 31(1):100-103
[21] Somashekaraiah B V, Padmajaes K, Prasad R K. Phytotoxicity of cadmium ions on germination seedlings of mung bean (Phaseolus vulgarize ):Involvement of lipid peroxides in chlorophyll degradation[J].Plant Physiol, 1992, 85(1):85-89
[22] 李元, 王焕校, 吴玉树.镉和铁对烟草一些生理指标的影响[J].生态学报, 1992, 12(2):147-154
[23] 王广林, 张金池, 王丽, 等.铜、锌胁迫对丁香蓼生理指标的影响[J].南京林业大学学报, 2009, 33(4):43-47
[24] 原海燕, 黄苏珍, 郭智, 等.锌对镉胁迫下马蔺生长、镉积累及生理抗性的影响[J].应用生态学报, 2007, 18(9):2111-2116
[25] Lolkema P C, Voijs R. Copper tolerance in Silene cucubalus: Subcellular distribution of copper and its effects on chloroplasts and plastocyanin synthesis[J].Planta, 1986, 167(1):30-36
[26] 龚红梅, 李卫国.锌对植物的毒害及机理研究进展[J].安徽农业科学, 2009, 37(29):14009-14015
[27] Ouzounidou G. Changes of photosynthetic activities in leaves as a result of Cu-treatment: Dose respon-se relations in Silene and Thlaspi[J].Photosynthetica, 1994, 29(3):455-462
[28] 王俊, 郭颖, 吴蕊, 等.不同种植年限和施肥量对日光温室土壤锌累积的影响[J].农业环境科学学报, 2009(1):93-98
[29] 甄泉, 严密, 杨红飞, 等.铜污染对野艾蒿生长发育的胁迫及伤害[J].应用生态学报, 2006, 17(8):1505-1510
[30] 储玲, 刘登义, 王友保, 等.铜污染对三叶草幼苗生长及活性氧代谢影响的研究[J].应用生态学报, 2004, 15(1):119-122
[31] Tei G M, Huchzermeyer B, Schultz G. Inhibition of chloroplast ATP synthase/ATP ase is a primary effect of heavy metal toxicity in spinach plants[J]. Biochemie Physiologie Pflanzen, 1990, 186(3):165-168
[32] Van A F V, Clij S. Effects of metals on enzyme activity in plants[J].Plant Cell Environment, 1990, 13(3):195-206

Effects of Cu²⁺and Zn²⁺ Stress on the Bulbil Germination and Physiological-biochemical Characteristics of Polygonum viviparum

铜、锌胁迫对珠芽蓼珠芽萌发及生理生化特性的影响

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WAN Ting, DUAN Jun-yi, LI Meng, CHEN Zhi-yong. Correlation Between MlNAC2 Gene Differential Expression and Salt-resistance Physiological Response in Miscanthus lutarioriparius Polyploid [J]. Acta Agrestia Sinica, 2021, 29(12): 2685-2693.
[2]	YU Shu-yan, ZHOU Yan-fei, HUANG Wei, WANG Xing, GAO Xue-qin, FU Bing-zhe. Effects of Drought,Salt,and Drought-salt Combined Stress on Photosynthetic and Physiological Characteristics of Agropyron mongolicum [J]. Acta Agrestia Sinica, 2021, 29(11): 2399-2406.
[3]	QI Chen-xi, YI Guan-tao, FU Xiao-xuan, WANG Hai-long, WANG Jia-lin, WU Jian-hui. Effects of Arbuscular Mycorrhizal Fungi on Physiological Indexes of Potentilla anserina under Drought-Stress [J]. Acta Agrestia Sinica, 2021, 29(11): 2407-2412.
[4]	LI Xuan, LIU Bo-wen, GAO Shuang-hong, YOU Xiang-kai, LONG Si, LI You-yue, LIU Qian, ZHANG Shi-jun, XU Yue-fei. Effects of Exogenous Hydrogen Sulfide on Morphology and Physiological Indexes of Tall Fescue under Low Light Stress [J]. Acta Agrestia Sinica, 2021, 29(11): 2435-2441.
[5]	PAN Xin-ya, LI Jun-bao, CHEN Yang, WANG Peng-fei, WEI Xian-wei, LI Rui, LIU Jia-xi, ZHENG Zhi-long, XU Bing-cheng, WANG Zhi. Response of Root Morphology and Anatomical Structure of Six Alfalfa Cultivars to Phosphorus Deficiency [J]. Acta Agrestia Sinica, 2021, 29(11): 2494-2504.
[6]	JIANG Hui-na, JING Song, LI Han-yu, SHE Mu-zi, ZHANG Xin-fei, HU Tian-ming, FU Juan-juan, MIAO Yan-jun. Cloning and Expression Analysis of EnPLA1 Gene in Tibetan wild Elymus nutans Griseb. [J]. Acta Agrestia Sinica, 2021, 29(10): 2141-2148.
[7]	ZHANG Xu-hui, MA Shao-ying, MA Lei, YANG Ning, WANG Na, ZHANG Wei-xia, LIAN Rong-fang, LI Sheng, CHAI Qiang. Effects of Rhizobium Inoculation on the Growth and Photosynthetic Characteristics of Pea Plants [J]. Acta Agrestia Sinica, 2021, 29(6): 1234-1241.
[8]	QIMEI La-mu, PUBU Zhuo-ma, CUI Tong-tong, ZHANG Xin-fei, JIANG Hui-na, FU Juan-juan, HU Tian-Ming. Effects of Exogenous Calcium on Physiology and Related Gene Expression of Elymus nutans under Cold Stress [J]. Acta Agrestia Sinica, 2021, 29(5): 919-928.
[9]	LIANG Jia, MAYINU·Wusiman, FANG Zhi-gang. Effects of Exogenous Growth Substances on Seed Germination of Sweet Sorghum under Drought Stress [J]. Acta Agrestia Sinica, 2021, 29(3): 610-617.
[10]	CHEN Bin, XUE Qing, LI Zi-wei, YANG Yu-jia, YANG Xiao-mei, BO Shan, LI Qiang, HE Miao. Effects of Light Intensities on Physiological Characteristics and Ultrastructure of Four Commelinaceae Plants [J]. Acta Agrestia Sinica, 2021, 29(2): 281-292.
[11]	DU Chong-xuan, LIU Yun-gen, WANG Yan, BAO Ning-ying, CHEN Tian. Effects of Arsenic Pollution on the Growth of Typha and the Secretion of N-alkanes in Roots [J]. Acta Agrestia Sinica, 2021, 29(2): 349-355.
[12]	ZHAO Li-qing, PENG Xiang-yong, LIU Jun-xiang, MAO Jin-mei, SUN Zhen-yuan. Effects of Glutathione(GSH)on Antioxidant Defense System of Lolium Perenne Seedlings under Lead Stress [J]. Acta Agrestia Sinica, 2020, 28(6): 1527-1534.
[13]	WANG Xiao-xue, LI Yue, ZHANG Bin, JIA Ju-qing, FENG Mei-chen, ZHANG Mei-jun, YANG Wu-de. Effects of Drought Stress and Rehydration on Root Growth and Physiological Characteristics of Oats [J]. Acta Agrestia Sinica, 2020, 28(6): 1588-1596.
[14]	FU Jing-yan, LIANG Lin-lin, ZHOU Min, LI Zhou. Effects of seeds soaking with γ-aminobutyric acid on germination and aluminium tolerance of white clover [J]. Acta Agrestia Sinica, 2020, 28(5): 1275-1284.
[15]	YU Xin-rong, ZHANG Zhi-wei, ZHOU Yu, LIU Peng, YAO Hong-bin, MIAO Yan-jun, FU Juan-juan. Effects of Melatonin on Growth and Antioxidant System of Perennial Ryegrass Seedlings Under Cold and Drought Stresses [J]. Acta Agrestia Sinica, 2020, 28(5): 1337-1345.