Roles of Achene Mucilage in Defensing Freezing Injury for the Seed Germination of Mirabilis himalaica

doi:10.11733/j.issn.1007-0435.2014.06.021

Acta Agrestia Sinica ›› 2014, Vol. 22 ›› Issue (6): 1281-1287.DOI: 10.11733/j.issn.1007-0435.2014.06.021

Previous Articles Next Articles

Roles of Achene Mucilage in Defensing Freezing Injury for the Seed Germination of Mirabilis himalaica

LU Xiao¹, LAN Xiao-zhong², YANG Feng-jiao¹, MA Shi-yao¹, LIU Sheng-li¹, YU Meng¹, HE Qian¹, CHEN Yu-zhen¹

1. College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Ministry of Education, Beijing Forestry University, Beijing 100083, China;
2. College of Food Science, Tibetan College of Agriculture and Husbantry, Linzhi, Tibet 860000, China

Received:2014-01-10 Revised:2014-03-04 Online:2014-12-15 Published:2014-12-01

喜马拉雅紫茉莉瘦果粘液对种子低温萌发的保护

卢骁¹, 兰小中², 杨凤娇¹, 马世尧¹, 刘胜利¹, 玉猛¹, 贺茜¹, 陈玉珍¹

1. 北京林业大学生物科学与技术学院林木育种国家工程实验室, 北京 100083;
2. 西藏农牧学院食品科学学院, 西藏林芝 860000

通讯作者: 陈玉珍
作者简介:卢骁(1988-),男,河北保定人,硕士研究生,主要从事植物生理生化研究,E-mail:luxiao830@163.com
基金资助:
国家科技支撑计划项目(2011BAI13B06)资助

Abstract

Abstract:

Achenes of Mirabilis himalaica, an alpine medical plant distributed in the Linzhi area (Elevation: 3000 m) of Tibet, produced a pectinaceous mucilage layer that could imbibe a large amount of water. Observations under stero microscope show that M. himalaica achene is composed of pericarp, seed coat, embryo and starch.The main aim of this study is to explore the role of mucilage in the seed germination of this alpine plant under freezing stress. Germination tests indicate that the optimum temperature of seed germination is 15℃. The activity of achene tissue is tested by triphenyl tetrazolium chloride (TTC) method. Results indicate that the starch of achenes seriously affects the accuracy of the TTC method. And the optimum incubation time of the study is 72 hours. The germination rates of seeds with or without mucilage (demucilaged) declined at -5℃ or -10℃. However, the germination rate of achene with mucilage was significantly higher than that of the demucilaged ones after freezing stress. Both germination tests and TTC method indicate that achene mucilage presumably plays an important role in the life cycle of M. himalaica by aiding germination in the stressful habitats of Tibet alpine environment.

Key words: Mirabilis himalaica, Achene, Mucilage, Freezing stress, Germination

摘要：

以采自西藏林芝的喜玛拉雅紫茉莉(Mirabilis himalaica)瘦果为材料,了解瘦果结构组成、淀粉颗粒对瘦果TTC活性检测的影响,及不同吸胀时间瘦果抵抗低温胁迫的效果,以揭示瘦果分泌粘液在萌发过程中的低温保护作用.结果表明:瘦果由果皮、种皮、胚和淀粉颗粒组成,淀粉影响了TTC检测细胞活性的准确性,并发现成熟瘦果有分泌粘液的特性;瘦果发芽的适温为15℃;TTC法检测瘦果细胞活力显示,吸胀72 h是研究低温胁迫对喜马拉雅紫茉莉瘦果萌发影响的较适宜时间;吸胀72 h的瘦果低温胁迫处理后再进行萌发试验,-5℃和-10℃处理的瘦果在发芽进程中后期有粘液瘦果发芽率比去掉粘液瘦果的发芽率高8.3%左右.

关键词: 喜玛拉雅紫茉莉, 瘦果, 粘液, 低温, 萌发

CLC Number:

Q945.34

LU Xiao, LAN Xiao-zhong, YANG Feng-jiao, MA Shi-yao, LIU Sheng-li, YU Meng, HE Qian, CHEN Yu-zhen. Roles of Achene Mucilage in Defensing Freezing Injury for the Seed Germination of Mirabilis himalaica[J]. Acta Agrestia Sinica, 2014, 22(6): 1281-1287.

卢骁, 兰小中, 杨凤娇, 马世尧, 刘胜利, 玉猛, 贺茜, 陈玉珍. 喜马拉雅紫茉莉瘦果粘液对种子低温萌发的保护[J]. 草地学报, 2014, 22(6): 1281-1287.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2014.06.021

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2014/V22/I6/1281

References

[1] 卫生部药典委员会. 中华人民共和国卫生部药品标准:藏药:第1册[M]. 中华人民共和国卫生部,1995:104
[2] 中国科学院西北高原生物研究所. 藏药志[M]. 西宁:青海人民出版社,1991:461
[3] 青海省药品检验所,青海省藏医药研究所. 中国藏药[M]. 第1卷.上海:上海科学技术出版社,1996:303
[4] 卢杰,兰小中. 拉萨市珍稀濒危藏药植物资源调查研究. 中国中药杂志,2013,38(1):127-132
[5] 辛福梅,臧建成,兰小中. 喜马拉雅紫茉莉种子化感作用初探[J]. 中国园艺文摘,2012(11):22-24
[6] 黄振英, Gutterman Y,胡正海,等. 白沙蒿种子萌发特性的研究I.粘液瘦果的结构和功能[J]. 植物生态学报,2001,25(1):22-28
[7] 刘晓风,谭敦炎. 荒漠植物种子粘液的生态学意义[J]. 植物学通报,2007,24(3):414 -424
[8] 伍晨曦,孙羽,冯固. 小车前种子表面粘液物质的吸水特性及其对种子在干旱环境中萌发的影响[J]. 生态学报,2009,29(4):1849-1858
[9] 国际种子检验协会(ISTA). 1996国际种子检验规程[M]. 农业部全国农作物种子质量监督检测中心,浙江大学种子科学中心,译. 北京:中国农业出版社,1999:151-1721
[10] 康永祥,贠玉洁,赵宝鑫,等. 毛梾种子萌发特性及其解除休眠技术研究[J]. 种子,2011,30(9)22-29
[11] 颜启传,黄亚军. 种子四唑测定手册[M]. 上海科技出版社,1992:133
[12] 程利红,段旭昌,张宗勤,等. 中国红豆杉种子超低温保存技术研究[J]. 西北农林科技大学学报:自然科学版,2012,40(2):72-78
[13] Steponkus P. Refinement of the TTC method of determing cold injury [J]. Plant Physiology,1967,42(10):1423-1426
[14] Gutterman Y, Shem-Tov S. Structure and function of mucilaginous seed coats of Plantago coronopus inhabiting the Negev Desert of Israel [J]. Israel Journal of Plant Sciences.1996,44(2/3):125-133
[15] Western T L, Skinner D J, Haughn G W. Differentiation of mucilage secretory cells of Arabidopsis seed coat [J]. Plant Physiology,2000,122 (2):345-355
[17] Huang Z Y, Gutterman Y. Comparison of germination strategies of Artemisia ordosica with its two congenera from deserts of China and Israel [J]. Acta Botanica Sinica,2000,42(1):71-80
[18] Haughn G. Chaudhury A Genetic analysis of seed coat development in Arabidopsis [J]. Trends in plant science,2005,10(10):472-477
[19] Huang Z Y, Gutterman Y. Germination of Artemisia sphaerocephala (Asteraceae), occurring in the sandy desert areas of Northwest China [J]. South African Journal of Botany,1999,65(3):187-196
[20] Gutterman Y. Strategies of seed dispersal and germination in plants inhabiting deserts [J]. Botanical Review,1994,60(4):373-425
[21] Tamara L W. The sticky tale of seed coat mucilages: Production, genetics, and role in seed germination and dispersal [J]. Seed Science Research,2012,22(1):1-25
[22] Yang X J, Dong M, Huang Z Y. Role of mucilage in the germination of Artemisia sphaerocephala (Asteraceae) achenes exposed to osmotic stress and salinity [J]. Plant Physiology and Biochemistry,2010,48(2):131-135
[23] Huang Z Y, Gutterman Y. Value of the mucilaginous pellicle to seeds of the sand-stabilizing desert woody shrub Artemisia sphaerocephala (Asteraceae) [J]. Trees,2004,18(6):669-676
[24] Gutterman Y, Witztum A, Evenari M. Seed dispersal and germination in Blepharis persica (Burm.) Kuntze [J]. Israel Journal of Botany,1968(16):213-234
[25] 潘春柳,邓志军,黄燕芬,等. 绞股蓝种子休眠机理及其破除方法研究[J]. 西北植物学报,2013,33(8):1658-1664
[26] 韩春艳,孙卫邦,董青松,等. 广州相思子和毛相思子种子活力 TTC 测定方法研究[J]. 种子,2011,30(7):13-16
[27] 史锋厚,喻方圆,沈永宝,等. 超低温贮藏对油松种子的影响[J]. 南京林业大学学报:自然科学版,2005,29(6):119-122
[28] 胡晋,徐湲,陈叶平,等. 作物种子生活力和活力的影响[J]. 浙江农业大学学报,1994,20(4):411-416
[29] 黄振英, Gutterman Y,胡正海,等. 白沙蒿种子萌发特性的研究II. 环境因素的影响[J].植物生态学报,2001,25(2):240-246
[30] 周芝琴,李廷山,武艳培,等. 长芒草种子适宜萌发条件[J]. 草业科学,2013,30(2):218-222
[31] 张海清,邹应斌,肖国超,等. 抗寒种衣剂对早籼稻秧苗抗寒性的影响及其作用机理的研究[J]. 中国农业科学,2006,39(11):2220-2227
[32] 朱玉龙,王玺. 水杨酸包衣对低温胁迫下玉米种子萌发及幼苗生长的影响[J]. 玉米科学,2013,21(4):68-71

Roles of Achene Mucilage in Defensing Freezing Injury for the Seed Germination of Mirabilis himalaica

喜马拉雅紫茉莉瘦果粘液对种子低温萌发的保护

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	XU Jing, LI Wen-long, WU Xin-yue, LUO Jia-ning, LIAO Yuan-cheng. Differentiation of Seed Germination Behavior in Alpine Meadow on the Tibetan Plateau along the Elevation Gradient [J]. Acta Agrestia Sinica, 2021, 29(S1): 10-18.
[2]	ZUO Lang, WANG Shu-sen, MA Ying-mei, WEN Su-ya-le-tu, BAI Gang, YU Xin-ming. Effect of Torch Tree Extract on Seeds Germination in Two Types of Turfgrass [J]. Acta Agrestia Sinica, 2021, 29(9): 1927-1933.
[3]	MA Yin-shan, CHANG Yan, WANG Han-rui, JIA Yun-feng, LI Wen-ying, QI Rui-qiang. The Allelopathy of Hyoscyamus niger Seed Extract on Seed Germination and Seedling Growth of Lolium perenne [J]. Acta Agrestia Sinica, 2021, 29(8): 1697-1703.
[4]	ZHANG Jin-hua, HE Jing, ZHANG Xiao-yan, ZHANG Shu-heng, ZHANG Heng, LI Yan-xiang. Three Forage Extracts on Seed Germination and Seedling Growth of Recipient Plants under Herbicide Stress [J]. Acta Agrestia Sinica, 2021, 29(8): 1719-1728.
[5]	LI Shan-kui, MENG Huan-wen, LI Yu-chen, ZHANG Hai-qing, BAI Xue, ZHANG Yu-kuan, ZHOU Hong-you, WANG QIan-jun, ZHANG Xiao-yu. Analysis of Allelopathic Potential of Sabina chinensis to Several Farmland Weeds and Crops [J]. Acta Agrestia Sinica, 2021, 29(8): 1769-1778.
[6]	MA Peng-cheng, XIE Quan-gang, ZHAO Xiang, DONG Qi-jun, ZHANG Ji-yu. Effects of Different Treatments on Seed Germination and Persistent Sepals of Lespedeza potaninii Vass [J]. Acta Agrestia Sinica, 2021, 29(8): 1828-1834.
[7]	HUANG Rong, YAO Bo, ZHANG Yu-juan, JIA Qian-ying, LI Xiang-yang, ZHANG Hong, ZHANG Zhen-fen. Effect of Pantoea agglomerans CQ10 Lipopolysaccharide on Seed Germination and Seedling Growth of Alfalfa [J]. Acta Agrestia Sinica, 2021, 29(5): 965-971.
[8]	CHEN Wei-dong, ZHANG Yu-xia, CONG Bai-ming, A Ma-er, TIAN Yong-lei, ZHANG Qing-xin, DU Xiao-yan. Effects of Potassium Fertilizer on MDA,SP Content and Antioxidant System of Alfalfa Root Neck [J]. Acta Agrestia Sinica, 2021, 29(4): 717-723.
[9]	TONG Li-Rong, WANG Juan, SONG Yu, NI Shun-Gang. Study on Pollen Germination Characteristics of Lespedeza davurica [J]. Acta Agrestia Sinica, 2021, 29(3): 457-464.
[10]	CHEN Li-qiang, BAI Xiao-ming. Changes of Seed Structure,Yield and Quality of Melilotus dentatus During the Ripening Process [J]. Acta Agrestia Sinica, 2021, 29(3): 465-471.
[11]	LIANG Jia, MAYINU·Wusiman, FANG Zhi-gang. Effects of Exogenous Growth Substances on Seed Germination of Sweet Sorghum under Drought Stress [J]. Acta Agrestia Sinica, 2021, 29(3): 610-617.
[12]	PAN Ping-xin, NI Qiang, MA Rui, YANG Yong-yi, MA Yan-jun. Effects of Different Salt Treatments on Seed Germination and Seedling Growth of Lycium ruthenicum Murr. [J]. Acta Agrestia Sinica, 2021, 29(2): 342-348.
[13]	YE Xiao-qian, LIU Rui-xi, FENG Jin-hui, DENG Shi-ming, LI Ji-tao, DENG Zhi-jun. Effect of Mucilage of Plantago asiatica Seeds on Its Deterioration and Germination Resistance [J]. Acta Agrestia Sinica, 2021, 29(12): 2728-2732.
[14]	WANG Fei, TAN Yi, LYU Ya-ru, SU Wen-xin, JIANG Wan-tong, LIU Yu-le, YAN Jun-xin. Effects of Salicylic Acid and Gibberellin Pretreatment on Seeds Germination of Hyssopus officinalis under NaCl Stress [J]. Acta Agrestia Sinica, 2021, 29(12): 2862-2870.
[15]	WANG Xing, SONG Ke-chen, XU Dong-mei, LI Yong-kang, SA Chun-ning, YANG Yue. Effects of Artificial Caragana intermedia on Seeds Germination and Seedlings Growth of Under-Crown Agropyron mongolicum in Desert Steppe [J]. Acta Agrestia Sinica, 2021, 29(11): 2470-2476.