Association Mapping of Cold Tolerance with Molecular Markers from Cold Responsive ESTs in Eremochloa ophiuroides

doi:10.11733/j.issn.1007-0435.2014.06.024

Acta Agrestia Sinica ›› 2014, Vol. 22 ›› Issue (6): 1301-1307.DOI: 10.11733/j.issn.1007-0435.2014.06.024

Previous Articles Next Articles

Association Mapping of Cold Tolerance with Molecular Markers from Cold Responsive ESTs in Eremochloa ophiuroides

WANG Peng-liang^1,2, WANG Hai-yan¹, LIU Jian-xiu³, WANG Xiu-e¹

1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China;
2. Guangxi Forestry Research Institute, Nanning, Guangxi 530001, China;
3. Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, Jiangsu Province 210014, China

Received:2014-01-14 Revised:2014-04-20 Online:2014-12-15 Published:2014-12-01

假俭草抗寒性状与低温胁迫响应EST分子标记的关联分析

王鹏良^1,2, 王海燕¹, 刘建秀³, 王秀娥¹

1. 南京农业大学作物遗传与种质创新国家重点实验室细胞遗传研究所, 江苏南京 210095;
2. 广西壮族自治区林业科学研究院, 广西南宁 530002;
3. 江苏省中国科学院植物研究所, 江苏南京 210014

通讯作者: 王秀娥
作者简介:王鹏良(1978-),男,浙江新昌人,博士,研究方向为植物分子细胞遗传学,E-mail: pengliang_wang@163.com
基金资助:
国家自然科学基金(30670200);广西自然科学基金(2012GXNSFBA053037);广西林科院基金项目(林科200807号)资助

Abstract

Abstract:

The genotyping data on the representative 96 accessions in China were obtained using the primers of the ESTs (Expressed Sequence Tags) derived from sorghum in NCBI and the SSH library induced by cold stress in centipedegrass. Population structure was firstly analyzed, then association mapping between EST molecular marker loci and the index of cold tolerance LT₅₀ was performed by using the TASSEL GLM program. The results indicated that 46 polymorphic genetic loci were amplified from the 17 primer pairs. Of them, CINAU114-900, CINAU115-1500, CINAU116-400 were significantly associated with the index of cold tolerance, LT₅₀. Their explained portion of phenotypic variation were 5.10%,4.04% and 4.59%, respectively. Phenotypic effects of the three LT₅₀ were 0.2518℃,-0.3121℃ and 0.2449℃, respectively. CINAU114-900 and CINAU116-400 decreased the cold tolerance, whereas CINAU115-1500 increased the cold tolerance.

Key words: Eremochloa ophiuroides, Cold tolerance, EST molecular marker, Association mapping

摘要：

为深入研究抗寒分子机理以及为分子标记辅助育种奠定基础,以具有代表性的96份种源为材料,利用假俭草(Eremochloa ophiuroides)抗寒品种TifBlair低温诱导后所得的EST及其近缘种高粱NCBI中低温胁迫响应的EST开发分子标记,对假俭草抗寒指标LT₅₀观测值和EST分子标记位点进行关联分析.结果表明:开发的17对引物在假俭草群体共扫描到46个多态遗传位点,其中CINAU114-900,CINAU115-1500和CINAU116-400位点与抗寒指标LT₅₀有显著关联,其表型变异的解释率分别为5.10%, 4.04%和4.59%;其LT₅₀表型效应分别为0.2518℃,-0.3121℃和0.2449℃.CINAU114-900和CINAU116-400位点提高假俭草对低温的敏感性,而CINAU115-1500位点可提高其抗寒性.

关键词: 假俭草, 抗寒性状, EST分子标记, 关联分析

CLC Number:

S432.4

WANG Peng-liang, WANG Hai-yan, LIU Jian-xiu, WANG Xiu-e. Association Mapping of Cold Tolerance with Molecular Markers from Cold Responsive ESTs in Eremochloa ophiuroides[J]. Acta Agrestia Sinica, 2014, 22(6): 1301-1307.

王鹏良, 王海燕, 刘建秀, 王秀娥. 假俭草抗寒性状与低温胁迫响应EST分子标记的关联分析[J]. 草地学报, 2014, 22(6): 1301-1307.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2014.06.024

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2014/V22/I6/1301

References

[1] Burton J H, Dudeck A E, Green R L. Plant breeding characteristics relating to improvement of centipedegrass[J].Soil Crop Science,1983,42(1):53-58
[2] Hanna W W. Centipedegrass diversity and vulunerabilty [J].Crop Science,1995,35(2):332-334
[3] 徐柱. 中国禾草志[M]. 呼和浩特:内蒙古出版社,1997
[4] 吴佳海,尚以顺,唐成斌. 优良天然草坪地被植物-假俭草的研究[J]. 中国园林,2000,16(9):76-78
[5] 宣继萍,郭海林,刘建秀,等. 中国假俭草种质资源抗寒性初步鉴定[J]. 草业学报,2003,12(6):110-114
[6] 王鹏良,陈启广,吕智鹏,等. 假俭草种源抗寒性鉴定及其变异分析[J]. 草地学报,2009,17(5):547-551
[7] Hanna W W, Dobson J, Duncan R R. Regist ration of 'TifBlair'Centipedegrass [J]. Crop Science,1997,37(3):1017
[8] Weaver K R, Callahan L M, Caetano-Anolles G, et al. DNA amplification fringerprinting and hybridization analysis of centipedegrass [J]. Crop science,1995,35(3):881-885
[9] Sayavedra-Soto L, Callahan L, Gresshoff P. DNA isolation, partial library construction and restriction fragment length polymorphisms (RFLP) of centipedegrass[J].Physiology and Molecular Biology of Plants,1997,3(1):31-37
[10] 宣继萍,高鹤,刘建秀. 中国假俭草居群遗传多样性研究 Ⅲ RAPD分析[J]. 草业学报,2005,14(4):47-52
[11] 杨水莲,刘卫东,马涛,等. 假俭草ISSR-PCR反应体系的建立与优化[J]. 草原与草坪,2009,132(1):11-14
[12] 郑轶琦,王志勇,郭海林,等. 正交设计优化假俭草SRAP-PCR反应体系及引物筛选[J]. 草业学报,2008,17(4):110-117
[13] 郑轶琦,宗俊勤,薛丹丹,等. SRAP分子标记在假俭草杂交后代真实性鉴定中的应用[J]. 草地学报,2009,17(2):135-140
[14] Zheng Y, Guo H, Zang G, et al. Genetic linkage maps of centipedegrass Eremochloa ophiuroides(Munro) Hack based on sequence-related amplified polymorphismand expressed sequence tag-simple sequence repeat markers [J]. Scientia Horticulturae,2013,156(1):86-92
[15] Andersen J R, Lubberstedt T. Functional markers in plants [J].Trends in Plant Science,2003,8(11):554-560
[16] Ingvarsson P K, Street N R. Association genetics of complex traits in plants [J]. New Phytologist,2011,189(4):909-922
[17] Flint-Garcia S A, Thuillet A C, Yu J, et al. Maize association population: A high-resolution platform for quantitative trait locus dissection [J]. Plant Journal,2005,44(6):1054-1064
[18] Yu J, Buckler E S. Genetic association mapping and genome organization of maize [J].Current Opinion in Biotechnology,2006,17(2):155-160
[19] Zhu C, Gore M, Buckler E S, et al. Status and prospects of association mapping in plants[J].The Plant Genome,2008,1(1):5-20
[20] Khan M A, Korban S S. Association mapping in forest trees and fruit crops [J].Journal of Experimental Botany,2012,63(11):4045-4060
[21] Skot L, Sackville Hamilton N R, Mizen S, et al. Molecular genecology of temperature response in Lolium perenne: 2. association of AFLP markers with ecogeography [J]. Molecular Ecology,2002,11(9):1865-1876
[22] Skot L, Humphreys M O, Armstead I, et al. An association mapping approach to identify flowering time genes in natural populations of Lolium perenne (L.) [J]. Molecular Breeding,2005,15(3):233-245
[23] Skot L, Humphreys J, Humphreys M O, et al. Association of candidate genes with flowering time and water-soluble carbohydrate content in Lolium perenne (L.) [J]. Genetics,2007,177(1):535-547
[24] Li Y, Böck A, Haseneyer G, et al. Association analysis of frost tolerance in rye using candidate genes and phenotypic data from controlled, semi-controlled, and field phenotyping platforms [J]. BMC Plant Biology,2011,11:146-159
[25] 朱根海,朱培仁. 小麦抗冻性的季节变化及温度对脱锻炼的效应[J]. 南京农学院学报,1984,7(2):9-16
[26] 盖钧镒. 试验统计方法[M]. 北京: 中国农业出版社,2000
[27] Gao D, Du F, Zhu Y. Low-background and high-resolution contracted silver-stained method in polyacrylamide gels electrophoresis [J]. HEREDITAS,2009,31(6):668-673
[28] Falush D, Stephens M,Pritchard J K. Inference of population structure using multilocus genotype data: Dominant markers and null alleles [J]. Molecular Ecology Notes,2007,7(4):1-5
[29] Breseghello F, Sorrells M E. Association mapping of kernel size and milling quality in wheat (Triticum aestivum L.) cultivars [J]. Genetics,2006,172(2):1165-1177
[30] Tanksley S D. Mapping polygenes [J].Annual Review of Genetics,1993,27:205-233
[31] Grattapaglia D, Sederoff R. Genetic linkage maps of Eucalyptus Grandis and Eucalyptus Urophylla using a pseudo-testcross mapping strategy and RAPD markers [J].Genetics,1994,137(4):1121-1137
[32] Gupta P K, Rustgi S, Kulwal P L. Linkage disequilibrium and association studies in higher plants: Present status and future prospects [J]. Plant Molecular Biology,2005,57(4):461-485
[33] Hanna W W, Burton G W. Cytology, Reproductive-behavior, and fertility characteristics of centipedegrass [J].Crop Science,1978,18(5):835-837
[34] Ponting R C, Drayton M C, Cogan N O, et al. SNP discovery, validation, haplotype structure and linkage disequilibrium in full-length herbage nutritive quality genes of perennial ryegrass (Lolium perenne L.) [J].Molecular Genetics and Genomics,2007,278(5):585-597
[35] Xing Y, Frei U, Schejbel B, et al. Nucleotide diversity and linkage disequilibrium in 11 expressed resistance candidate genes in Lolium perenne [J]. BMC Plant Biology,2007,7(1):43-54
[36] Neale D B, Ingvarsson P K. Population, quantitative and comparative genomics of adaptation in forest trees [J]. Current Opinion in Plant Biology,2008,11(2):149-155
[37] Brown G R, Gill G P, Kuntz R J, et al. Nucleotide diversity and linkage disequilibrium in loblolly pine [J]. Proceedings of the National Academy of Sciences of the United States of America,2004,101(42):15255-15260
[38] Krutovsky K V, Neale D B. Nucleotide diversity and linkage disequilibrium in cold-hardiness and wood quality-related candidate genes in Douglas fir [J]. Genetics,2005,171(4):2029-2041
[39] Tenaillon M I, Sawkins M C, Long A D, et al. Patterns of DNA sequence polymorphism along chromosome 1 of maize (Zea mays ssp. mays L.) [J]. Proceedings of the National Academy of Sciences of the United States of America,2001,98(16):9161-9166
[40] Remington D L, Thornsberry J M, Matsuoka Y, et al. Structure of linkage disequilibrium and phenotypic associations in the maize genome [J]. Proceedings of the National Academy of Sciences of the United States of America,2001,98(20):11479-11484
[41] Ching A, Caldwell K S, Jung M, et al. SNP frequency, haplotype structure and linkage disequilibrium in elite maize inbred lines [J]. BMC Genetics,2002,3:19-32
[42] Flint-Garcia S A, Thornsberry J M, Buckler E S. Structure of linkage disequilibrium in plants [J].Annual Review of Plant Biology,2003,54:357-374
[43] Thornsberry J M, Goodman M M, Doebley J, et al. Dwarf8 polymorphisms associate with variation in flowering time [J].Nature Genetics,2001,28(3):286-289
[44] Wilson L M, Whitt S R, Ibanez A M, et al. Dissection of maize kernel composition and starch production by candidate gene association [J]. Plant Cell,2004,16(10):2719-2733

Association Mapping of Cold Tolerance with Molecular Markers from Cold Responsive ESTs in Eremochloa ophiuroides

假俭草抗寒性状与低温胁迫响应EST分子标记的关联分析

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 6

Recommended Articles

Metrics

Comments

[1]	JIA Xiang, DUOJI Ge-sang, ZHAO Ai-min, ZHANG Wen-cai, QUNI Jia-cuo, LABA Zhuo-ma, PUBU Ci-reng, MIAO Yan-jun. Comprehensive Evaluation of Cold resistance of 4 Species of Gramineae at Seedling Stage [J]. Acta Agrestia Sinica, 2020, 28(5): 1372-1378.
[2]	WU Xue-li, GUO Zhen-fei, CHEN Shen-miao, ZHUANG Li-li. Advances in Research on the Tolerance of Seashore Paspalum (Paspalums vaginatium) [J]. Acta Agrestia Sinica, 2019, 27(5): 1117-1125.
[3]	LIU Yu-shi, GENG Jin-cai, SONG Jiang-hu, SHA Xu-yang, HU Tian-ming, YANG Pei-zhi. Effect of Root Nodules on Cold Resistance of Alfalfa (Medicago sativa) [J]. Acta Agrestia Sinica, 2018, 26(3): 631-638.
[4]	Wang Peng-liang, Chen Qi-guang, Lü Zhi-peng, Wen Ming-xing, Wang Hai-yan, Qin Zi-hai, Liu Jian-xiu, Wang Xiu-e. Evaluation and variation analysis of cold tolerance in Eremochloa ophiuroides [J]. , 2009, 17(5): 547-551.
[5]	GUO Hai-lin, LIU Jian-xiu, GUO Ai-gui, MA Ke-qun. Comprehensive Evaluation on the Mutant Progenies of Hybrid Bermudagrasses [J]. , 2008, 16(2): 145-149,163.
[6]	ZHANG Yan-qin, JIA Wei-long, YANG Li-li, GUO Xian-long, WU Qi, HU Yuan-lei, LIN Zhong-ping, WANG Miao, XU Long-jun . Induction and Identification of Cold Tolerance for Festuca Arundinacea [J]. , 2006, 14(2): 124-128.