›› 2009, Vol. 17 ›› Issue (5): 547-551.DOI: 10.11733/j.issn.1007-0435.2009.05.002

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Evaluation and variation analysis of cold tolerance in Eremochloa ophiuroides

Wang Peng-liang1,3, Chen Qi-guang1, Lü Zhi-peng1, Wen Ming-xing1, Wang Hai-yan1, Qin Zi-hai3, Liu Jian-xiu2, Wang Xiu-e1   

  1. 1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing 210095, China;
    2. Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China;
    3. Guangxi Forestry Research Institute, Nanning 530001, China
  • Received:2009-02-24 Revised:2009-06-23 Online:2009-10-15 Published:2009-10-15

假俭草种源抗寒性鉴定及其变异分析

王鹏良1,3, 陈启广1, 吕志鹏1, 温明星1, 王海燕1, 覃子海3, 刘建秀2, 王秀娥1   

  1. 1. 南京农业大学作物遗传与种质创新国家重点实验室, 南京农业大学细胞遗传研究所, 南京, 210095;
    2. 江苏省中国科学院植物研究所, 南京, 210014;
    3. 广西壮族自治区林业科学研究院, 南宁, 530001
  • 通讯作者: 王秀娥,E-mail:xiuew@njau.edu.cn
  • 作者简介:王鹏良(1978- ),男,浙江新昌人,博士研究生,研究方向为植物分子细胞遗传学,E-mail:pengliang_wang@yahoo.com.cn
  • 基金资助:
    国家自然科学基金(30670200);广西林科院基金项目(林科200807号)

Abstract: In order to identify germplasms with cold tolerance in centipedegrass,and provide useful materials and information for further genetic and breeding research,110 centipedegrass cultivars were evaluated for cold tolerance using an electrolyte leakage method.The results indicated that significant genetic differences were present among different varieties in centipedegrass.The LT50 of 110 centipedegrasses ranged from-2.55 to-8.01,with a range of 5.46℃,an average LT50 of-4.89℃,and a coefficient variation of 18.01%.No significant linear correlations were observed between LT50 of centipedegrass and geographical factors(latitude,longitude and elevation).The 110 cultivars were subdivided into three groups by cluster analysis based on their LT50.

Key words: Eremochloa ophiuroides, Cold tolerance, LT50, Regression analysis, Cluster analysis

摘要: 为了进一步鉴定和发掘假俭草抗寒遗传资源,为假俭草抗寒育种和遗传研究提供材料和有价值的信息,本研究采用电解质渗透法对搜集的有代表性的110份假俭草种源进行了抗寒性鉴定。结果表明,我国假俭草不同种源之间的抗寒性存在较大变异,它们的半致死温度的变异范围为-2.55℃~-8.01℃,极差为5.46℃,平均半致死温度为-4.89℃,变异系数为18.01%;进一步对假俭草的半致死温度与地理因子(纬度,经度和海拔)进行了回归分析,结果发现假俭草的半致死温度与纬度,经度和海拔均无线性关系;根据假俭草的半致死温度进行系统聚类分析,可以将110份假俭草种源分为3个类群。

关键词: 假俭草, 抗寒性, 半致死温度, 回归分析, 系统聚类分析

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