Physiological Response of Medicago sativa to Odontothrips loti Damage

doi:10.11733/j.issn.1007-0435.2015.01.023

Acta Agrestia Sinica ›› 2015, Vol. 23 ›› Issue (1): 143-150.DOI: 10.11733/j.issn.1007-0435.2015.01.023

Previous Articles Next Articles

Physiological Response of Medicago sativa to Odontothrips loti Damage

KOU Jiang-tao, SHI Shang-li

College of Grassland Science, Gansu Agricultral University, Key Laboratory of Ecosystem Ministry of Education, Pratacultural Engineering Laboratory of Gansu Province, Sino-Us Center for Grazingland Ecosystem Sustainability, Lanzhou, Gansu Province 730070, China

Received:2014-03-14 Revised:2014-06-04 Online:2015-02-15 Published:2015-01-31

紫花苜蓿对牛角花齿蓟马为害的生理响应研究

寇江涛, 师尚礼

甘肃农业大学草业学院草业生态系统教育部重点实验室甘肃省草业工程实验室中-美草地畜牧业可持续研究中心, 甘肃兰州 730070

通讯作者: 师尚礼
作者简介:寇江涛(1986-),男,甘肃镇原人,博士,研究方向为草种质资源及育种、牧草病虫害防治,E-mail:koujingtao@st.gsau.edn.cn
基金资助:
国家现代牧草产业体系建设专项(CARA-35)资助

Abstract

Abstract:

In order to clarify the physiological resistance mechanism of alfalfa plant to thrip damage, the soluble protein contents, free proline contents as well as the SOD, GPX and CAT activities of resistant and susceptible alfalfa R-1 and I-1 clones growing under different thrip densities were investigated. The results indicated that the SOD and GPX activities of R-1 clones increased under low insect densities (1, 3 per branch), whereas the SOD and GPX activities of R-1 clones increased firstly and then decreased under high insect densities (5, 7 per branch); The SOD and GPX activities of I-1 treatments increased firstly and then decreased under all tested conditions. The soluble protein contents and CAT activities of R-1 and I-1 treatments decreased, whereas the free proline contents R-1 and I-1 treatments increased under all tested conditions. The CAT activities, soluble protein and free proline contents of R-1 clones changed less than that of I-1 clones under all tested conditions, indicating that the increases of SOD and GPX activities were the physiological response of alfalfa plant to thrip damage. R-1 clone under low thrip densities was resistant to thrip damage that manifested in the continuous increases of SOD and GPX activities, while I-1 clone was susceptible to thrip compared with R-1 clone.

Key words: Medicago sativa, Odontothrips loti, Physiological response

摘要：

为明确紫花苜蓿(Medicago sativa)对牛角花齿蓟马(Odontothrips loti)为害的抗性生理机理,测定了不同牛角花齿蓟马虫口密度下抗蓟马苜蓿无性系R-1、感蓟马苜蓿无性系I-1可溶性蛋白、游离脯氨酸含量及超氧化物歧化酶(SOD)、愈创木酚过氧化物酶(GPX)、过氧化氢酶(CAT)活性的变化.结果表明:受牛角花齿蓟马为害后,R-1无性系在低虫口密度(1, 3头·枝条^-1)下,SOD, GPX活性升高,在高虫口密度(5, 7头·枝条^-1)下,SOD, GPX活性先上升后下降;I-1无性系各接虫处理的SOD, GPX活性均先上升后下降;二者各接虫处理的可溶性蛋白含量均降低,游离脯氨酸含量均增加,CAT活性均下降,且在牛角花齿蓟马为害过程中,R-1无性系各接虫处理可溶性蛋白的减少量、游离脯氨酸的增加量及CAT活性的下降速率均小于I-1无性系.说明SOD, GPX活性的升高是紫花苜蓿对牛角花齿蓟马诱导抗性的一种表现,R-1无性系在低虫口密度下其SOD, GPX活性持续升高而表现为抗蓟马,I-1无性系对蓟马为害的诱导抗性反应滞后于R-1无性系而表现为感蓟马.

关键词: 紫花苜蓿, 牛角花齿蓟马, 生理响应

CLC Number:

S433

KOU Jiang-tao, SHI Shang-li. Physiological Response of Medicago sativa to Odontothrips loti Damage[J]. Acta Agrestia Sinica, 2015, 23(1): 143-150.

寇江涛, 师尚礼. 紫花苜蓿对牛角花齿蓟马为害的生理响应研究[J]. 草地学报, 2015, 23(1): 143-150.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2015.01.023

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2015/V23/I1/143

References

[1] 洪绂曾. 苜蓿科学[M]. 北京:中国农业出版社,2009
[2] 吴永敷,赵秀华,特木尔布和. 蓟马是我国苜蓿生产的主要害虫[J]. 中国草地,1990(3):65-66
[3] 吴永敷,赵秀娴,赵秀华,等. 蓟马对苜蓿的危害[J]. 中国草地,1988(2):25-27
[4] 张蓉,马建华,王进华,等. 宁夏苜蓿病虫害发生现状及防治对策[J]. 草业科学,2003,20(6):40-44
[5] 严林,梅洁人. 青海省紫花苜蓿病虫种类及害虫天敌的调查[J]. 植物保护,1996,22(5):24-25
[6] 贺春贵,王森山,曹致中,等. 40个苜蓿品种(系)对蓟马田间抗性评[J]. 草业学报,2007,16(5):79-83
[7] 胡桂馨,贺春贵,王森山,等. 不同苜蓿品种对牛角花齿蓟马的抗性机制初步研究[J]. 草业科学,2007,24(9):86-89
[8] 胡桂馨,师尚礼,王森山,等. 不同苜蓿品种对牛角花齿蓟马的耐害性研究[J]. 草地学报,2009,17(4):505-509
[9] 寇江涛,胡桂馨,张新颖,等. 持续危害下抗、感蓟马苜蓿无性系大田生长特性的比较[J]. 草原与草坪,2011,31(4):35-40
[10] 寇江涛,师尚礼,胡桂馨,等. 抗、感蓟马苜蓿无性系对蓟马危害的补偿光合生理反应比较[J]. 昆虫学报,2011,54(8):910-917
[11] 陈建明,俞晓平,程家安,等. 植物耐虫性研究进展[J]. 昆虫学报,2005,48(2):262-272
[12] 陈青,张银东. 3种氧化酶与辣椒抗蚜性的相关性[J]. 热带作物学报,2004,25(3):42-46
[13] 贾贞,宋占午,金祖荫,等. 山楂叶螨危害对海棠叶片POD的影响[J]. 西北植物学报,2004,24(11):2136-2139
[14] 张金锋,薛庆中. 稻飞虱胁迫对水稻植株内主要保护酶活性的影响[J]. 中国农业科学,2004,37(10):1487-1491
[15] 吴青君,龚佑辉,徐宝云. 西花蓟马主要寄主植物可溶性糖和蛋白质含量测定[J]. 中国蔬菜,2007(10):20-22
[16] Bernays E A, Chapman R F. Host-plant selection by phytophagous insects [M]. London:United Kingdom,1994
[17] 刘裕强,江玲,孙立宏,等. 褐飞虱刺吸诱导的水稻一些防御性酶活性的变化[J]. 植物生理与分子生物学学报,2005,31(6):643-650
[18] 邢会琴,李敏权,徐秉良,等. 过氧化物酶和苯丙氨酸解氨酶与苜蓿白粉病抗性的关系[J]. 草地学报,2007,7(4):376-380
[19] 黄伟,贾志宽,韩清芳. 蚜虫(Aphis medicaginis Koch)危害胁迫对不同苜蓿品种体内丙二醛含量及防御性酶活性的影响[J]. 生态学报,2007,27(6):2177-2183
[20] 武德功,贺春贵,刘长仲,等. 不同苜蓿品种对豌豆蚜的生化抗性机制[J]. 草地学报,2011,19(3):497-501
[21] 武德功,王森山,刘长仲,等. 豌豆蚜刺吸胁迫对不同苜蓿品种体内单宁含量及生理活性的影响[J]. 草地学报,2011,19(2):351-355
[22] 邹琦. 植物生理学实验指导[M]. 北京:中国农业出版社,2006
[23] André Dias de Azevedo Neto, José Tarquinio Prisco, Joaquim Enéas-Filho, et al. Hydrogen peroxide pre-treatment induces salt-stress acclimation in maize plants [J]. Journal of Plant Physiology,2005,162(10):1114-1122
[24] Giannopolitis C N, Ries S K. Superoxide dismutases. I. Occurrence in higher plants [J]. Plant Physiology,1977,59(2):309-314
[25] Urbanek H, Kuzniak-Gebarowska E, Herka K. Elicitation of defense responses in bean leaves by Botrytis cinerea polygalacturonase [J]. Acta Physiologiae plantarum,1991,13(1):43-50
[26] Beers J R F, Sizer I W. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase [J]. Journal of Biological Chemistry,1952,195(1):133-40
[27] 王娟,李德全. 逆境条件下植物体内渗透调节物质的积累与活性氧代谢[J]. 植物学通报,2001,18(4):459-465
[28] 贺志理,王洪春. 盐胁迫下苜蓿中盐蛋白的诱导产生[J]. 植物生理学报,1991,17(1):71-79
[29] 汤章城. 逆境条件下植物脯氨酸的累积及其可能的意义[J]. 植物生理学通讯,1984,17(1):15-21
[30] Ashraf M, Mueen U D M, Warrich N. Production efficiency of mung bean (Vigna radiata L.) as affected by seed inoculation and NPK application [J]. International Journal Agriculture Biology,2003,5(2):179-180
[31] Cernusak L A, Aranda J, Marshall J D, et al. Large variation in whole plant water use efficiency among tropical tree species [J]. New Phytologist,2007,173(2):294-305
[32] Mc Neill S, Southwood T R E. Role of nitrogen in the development of insect-plant relationships [M]. Harbourne Biochemical Journal. Biochemical Aspects of Plant and Animal Coevolution. New York:Academic Press,1978:77-98
[33] William J, Mattson J. Herbivory in relation to plant nitrogen content [J]. Annual Review of Ecology and Systematics,1980,102(11):119-161
[34] 刘玉良,米福贵,特木尔布和,等. 苜蓿蓟马抗性与生理活性相关性研究[J]. 安徽农业科学,2009,37(18):8569-8571,8613
[35] 陈成升,谢志霞,刘小京. 旱盐互作对冬小麦幼苗生长及其抗逆生理特性的影响[J]. 应用生态学报,2009,20(4):811-816
[36] Wang X, Wang H, Wu F, et al. Effects of cinnamic acid on the physiological characteristics of cucumber seedlings under salt stress [J]. Front Agriculture China,2007,1(1):58-61
[37] 高必达,陈捷. 生理植物病理学[M]. 北京:科学出版社,2006
[38] Bowler C, Montagu M V, Inze D. Superoxide dismutase and stress tolerance [J]. Annual Review of Plant Physiology and Plant Molecular Biology,1992,43(1):83-116
[39] Bruce R J, West C A. Elicitation of lignin biosynthesis and isoperoxidase activity by pectic fragnents in suspension cultures caster bean [J]. Plant Physiology,1989,91(3):889-897
[40] Chen Z, Klessig D F. Identification of a soluble salicylic acidbinding protein thatmay function in signal transduction in the plant disease resistance response [J]. Proceedings of the National Academy of Science of USA,1991,88(18):8179-8183

Physiological Response of Medicago sativa to Odontothrips loti Damage

紫花苜蓿对牛角花齿蓟马为害的生理响应研究

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LI Hong, LI Bo, FANG Zhi-jian, YANG Zhao, ZHANG Chao, FENG Cheng-long, JIN Shan-shan, WANG Si-qi, SHI Wan-ying. Physiological Response of Alfalfa Seedlings to Freeze-thaw or Freeze-thaw Versus Soda Saline-alkali Stress [J]. Acta Agrestia Sinica, 2021, 29(7): 1469-1476.
[2]	WAN Ting, DUAN Jun-yi, LI Meng, CHEN Zhi-yong. Correlation Between MlNAC2 Gene Differential Expression and Salt-resistance Physiological Response in Miscanthus lutarioriparius Polyploid [J]. Acta Agrestia Sinica, 2021, 29(12): 2685-2693.
[3]	JIA Xiang-yang, CHONG Pei-fang, ZHANG Yu-jie, LI Yi, SU Shi-ping. Effects of Nitric Oxide on Physiological Characteristics and Growth of Reaumuria soongorica Seedling under NaCl Stress [J]. Acta Agrestia Sinica, 2019, 27(3): 628-636.
[4]	SONG Ya-li, WANG Ke-qin, WANG Sha, QIAN Xia-ji, XU Jing. Physiological Responses of Three Kinds of Cool Season Turfgrasses under Continuous Drought Stress, Heat Stress and Their Interaction [J]. Acta Agrestia Sinica, 2018, 26(3): 705-717.
[5]	HUANG Chun-qiong, CHEN Zhen, LIU Guo-dao, BAI Chang-jun. The Physiological Response of the Warm-season Turfgrass Cynodon dactylon Accessions to Acidity and Aluminum Stress [J]. Acta Agrestia Sinica, 2017, 25(4): 796-802.
[6]	LI Xiao-yu, MU Chun-sheng. Effects of Salt and Alkali Stresses, and Exogenous Plant Hormones on Growth and Development of Wheat and L.chinensis [J]. Acta Agrestia Sinica, 2017, 25(2): 257-260.
[7]	XIE Nan, ZHAO Hai-ming, LI Yuan, You Yong-liang, LI Wei, LIU Gui-bo, LIU Hong-cai. Salt Tolerance Evaluation and Physiological Responses of Forage Rye and Triticale at Seedling Growth Stages [J]. Acta Agrestia Sinica, 2016, 24(1): 84-92.
[8]	ZHU Hui-sen, WANG Tai-liang, ZHANG Yan, HAN Zhao-sheng, DONG Kuan-hu. Effects of Winter Cover on the Morphological and Physiological Characteristics of Shanxi Wild Bermudagrass [J]. Acta Agrestia Sinica, 2015, 23(2): 346-351.
[9]	LEI Ya-wei, BAI Xiao-ming, WANG Ting, LU You-wei, LEI Shu-han. Alleviation Effect of ABA on the Wild Kentucky Bluegrasses under Heat Stress [J]. Acta Agrestia Sinica, 2015, 23(1): 89-94,100.
[10]	WU Jian-hui, LI Xue, WANG Ling. The Physiological Response of Anatomical Structures and Physiological Indexes of Two Potentilla Species to Drought Stress [J]. Acta Agrestia Sinica, 2015, 23(1): 125-129.
[11]	JIANG Jia-lin, SUN Yan. Ecophysiological Responses of Carex Rigescens from Different Regions to High Temperature Stress [J]. , 2013, 21(3): 539-545.
[12]	XIE Xiao-rong, LI Wei, LIU Jin-rong, DU Jian-xiong. Physiological Evaluation of Drought and Heat Resistance for 21 Tall Fescue Turfgrass [J]. , 2009, 17(2): 202-205.
[13]	HE Hui-rong, LI Zhi-dong, ZHOU Er-wei, LIAO Wei-qing, LI Ke-hua, GUO Zhen-fei. Physiological Responses to Chilling of Six Warm-season Turfgrasses [J]. , 2008, 16(2): 150-153.
[14]	XIE Xiao-rong, LIU Jin-rong, LI Wei, SUN Ji-xiong, DU Jian-xiong. Physiological Response and Performance Evaluation of 18 Kentucky Bluegrass Turfgrasses under Drought and Heat Stress [J]. , 2007, 15(6): 572-576.
[15]	WANG Li, YANG Yun-fei, SUN Wei, WANG De-li. Photosynthetic Physiological Response of Two Ecotypes of Leymus chinensis to the Double Concentration of CO₂ [J]. , 2003, 11(1): 52-57.