Acta Agrestia Sinica ›› 2015, Vol. 23 ›› Issue (2): 302-309.DOI: 10.11733/j.issn.1007-0435.2015.02.013

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Effects of Intercropping between Mulberry and Alfalfa on Soil Enzyme Activities and Microbial Community Diversity in Rhizophere

ZHANG Meng-meng1, AO Hong1, LI Xin1, ZHANG Jing-yun2, WANG Ning1, JU Cheng-mei2, WANG Jia1, CAI Dun-jiang2, SUN Guang-yu1   

  1. 1. College of Life Science, Northeast Forest University, Harbin, Heilongjiang Province 150040, China;
    2. Institute of Crops, Heilongjiang Academy of Land Reclamation and Agricultural Sciences, Jiamusi, Heilongjiang Province 154007, China
  • Received:2014-04-17 Revised:2014-12-11 Online:2015-04-15 Published:2015-03-25

桑树/苜蓿间作对根际土壤酶活性和微生物群落多样性的影响

张萌萌1, 敖红1, 李鑫1, 张景云2, 王宁1, 鞠成梅2, 王佳1, 蔡敦江2, 孙广玉1   

  1. 1. 东北林业大学生命科学学院, 黑龙江 哈尔滨 150040;
    2. 黑龙江省农垦科学院作物所, 黑龙江 佳木斯 154007
  • 通讯作者: 孙广玉, 敖红
  • 作者简介:张萌萌(1989-),女,山东菏泽人,硕士研究生,主要从事植物营养方面的科研工作.E-mail:18686893084@163.com
  • 基金资助:

    科技部国家科技支撑项目(2011BAD08B02-3);黑龙江省自然科学基金重点项目(ZD201105)资助

Abstract:

The effects of intercropping between mulberry and alfalfa on the enzyme activities and functional diversities of rhizophere soil microbial community were investigated comparing with mulberry monoculture and alfalfa monoculture in the field experiment. The results showed that the invertase and peroxidase activities of intercropping alfalfa increased 30.0% and 21.4% compared to monoculture alfalfa, respectively; the acid phosphatase activities of intercropping mulberry and intercropping alfalfa raised 23.0% and 28.9% compared to monocultures, respectively. The activities of the enzymes were significant differences between monoculture and intercropping. In mulberry and alfalfa intercropping system, average well color development (AWCD) symbolized the metabolic activities of soil microbes. Shanon index, Simpson index, and McIntosh index of intercropping mulberry were higher than that of monoculture, while theses indexes in intercropping alfalfa were lower than that of monoculture. Principal component analysis (PCA) showed that the main carbon source types of intercropping and monoculture were different, the main carbon sources of intercropping alfalfa changed into amino acid, polymers and miscellaneous. The research indicated that the intercropping system enhanced the diversity of rhizophere soil microbial community of mulberry, and changed the main carbon source types of rhizophere soil microbial community of alfalfa. The effects of intercropping patterns on soil enzyme activities were different.

Key words: Intercropping, Soil enzyme activity, Soil microbial community, BiologMT

摘要:

以桑树(Morus alba)/苜蓿(Medicago sativa)间作系统为研究对象,探讨间作种植模式对根际土壤酶活性及土壤微生物群落功能多样性的影响.结果表明:间作苜蓿的蔗糖酶和过氧化氢酶活性分别比单作苜蓿提高了30.0%和21.4%,达显著差异水平;而间作桑树则相反,分别比单作桑树降低了23.8%和2.6%,达显著差异水平;间作桑树和间作苜蓿的根际土壤酸性磷酸酶活性分别比单作提高了23.0%和28.9%,而脲酶活性则分别降低了52.4%和44.3%,二者差异显著.在桑树/苜蓿间作体系下,间作桑树的表征土壤微生物代谢活性强弱平均颜色变化率、多样性指数、均匀度指数和优势度指数均显著高于单作桑树,而间作苜蓿均低于单作苜蓿,达显著差异水平.主成分分析表明,间作种植模式改变了作物根际土壤微生物群落的主要碳源类型,使苜蓿的主要碳源变为氨基酸、聚合物和其他化合物,与单作之间具有显著差异.表明桑树/苜蓿间作提高了桑树根际土壤微生物群落的多样性,改变了苜蓿根际土壤微生物群落利用主要碳源的类型.

关键词: 间作, 土壤酶活性, 土壤微生物群落, BiologMT

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