Effects of Intercropping between Mulberry and Alfalfa on Soil Enzyme Activities and Microbial Community Diversity in Rhizophere

doi:10.11733/j.issn.1007-0435.2015.02.013

Acta Agrestia Sinica ›› 2015, Vol. 23 ›› Issue (2): 302-309.DOI: 10.11733/j.issn.1007-0435.2015.02.013

Effects of Intercropping between Mulberry and Alfalfa on Soil Enzyme Activities and Microbial Community Diversity in Rhizophere

ZHANG Meng-meng¹, AO Hong¹, LI Xin¹, ZHANG Jing-yun², WANG Ning¹, JU Cheng-mei², WANG Jia¹, CAI Dun-jiang², SUN Guang-yu¹

1. College of Life Science, Northeast Forest University, Harbin, Heilongjiang Province 150040, China;
2. Institute of Crops, Heilongjiang Academy of Land Reclamation and Agricultural Sciences, Jiamusi, Heilongjiang Province 154007, China

Received:2014-04-17 Revised:2014-12-11 Online:2015-04-15 Published:2015-03-25

桑树/苜蓿间作对根际土壤酶活性和微生物群落多样性的影响

张萌萌¹, 敖红¹, 李鑫¹, 张景云², 王宁¹, 鞠成梅², 王佳¹, 蔡敦江², 孙广玉¹

1. 东北林业大学生命科学学院, 黑龙江哈尔滨 150040;
2. 黑龙江省农垦科学院作物所, 黑龙江佳木斯 154007

通讯作者: 孙广玉, 敖红
作者简介:张萌萌(1989-),女,山东菏泽人,硕士研究生,主要从事植物营养方面的科研工作.E-mail:18686893084@163.com
基金资助:
科技部国家科技支撑项目(2011BAD08B02-3);黑龙江省自然科学基金重点项目(ZD201105)资助

Abstract

Abstract:

The effects of intercropping between mulberry and alfalfa on the enzyme activities and functional diversities of rhizophere soil microbial community were investigated comparing with mulberry monoculture and alfalfa monoculture in the field experiment. The results showed that the invertase and peroxidase activities of intercropping alfalfa increased 30.0% and 21.4% compared to monoculture alfalfa, respectively; the acid phosphatase activities of intercropping mulberry and intercropping alfalfa raised 23.0% and 28.9% compared to monocultures, respectively. The activities of the enzymes were significant differences between monoculture and intercropping. In mulberry and alfalfa intercropping system, average well color development (AWCD) symbolized the metabolic activities of soil microbes. Shanon index, Simpson index, and McIntosh index of intercropping mulberry were higher than that of monoculture, while theses indexes in intercropping alfalfa were lower than that of monoculture. Principal component analysis (PCA) showed that the main carbon source types of intercropping and monoculture were different, the main carbon sources of intercropping alfalfa changed into amino acid, polymers and miscellaneous. The research indicated that the intercropping system enhanced the diversity of rhizophere soil microbial community of mulberry, and changed the main carbon source types of rhizophere soil microbial community of alfalfa. The effects of intercropping patterns on soil enzyme activities were different.

Key words: Intercropping, Soil enzyme activity, Soil microbial community, Biolog^MT

摘要：

以桑树(Morus alba)/苜蓿(Medicago sativa)间作系统为研究对象,探讨间作种植模式对根际土壤酶活性及土壤微生物群落功能多样性的影响.结果表明:间作苜蓿的蔗糖酶和过氧化氢酶活性分别比单作苜蓿提高了30.0%和21.4%,达显著差异水平;而间作桑树则相反,分别比单作桑树降低了23.8%和2.6%,达显著差异水平;间作桑树和间作苜蓿的根际土壤酸性磷酸酶活性分别比单作提高了23.0%和28.9%,而脲酶活性则分别降低了52.4%和44.3%,二者差异显著.在桑树/苜蓿间作体系下,间作桑树的表征土壤微生物代谢活性强弱平均颜色变化率、多样性指数、均匀度指数和优势度指数均显著高于单作桑树,而间作苜蓿均低于单作苜蓿,达显著差异水平.主成分分析表明,间作种植模式改变了作物根际土壤微生物群落的主要碳源类型,使苜蓿的主要碳源变为氨基酸、聚合物和其他化合物,与单作之间具有显著差异.表明桑树/苜蓿间作提高了桑树根际土壤微生物群落的多样性,改变了苜蓿根际土壤微生物群落利用主要碳源的类型.

关键词: 间作, 土壤酶活性, 土壤微生物群落, Biolog^MT

CLC Number:

Q945.79

ZHANG Meng-meng, AO Hong, LI Xin, ZHANG Jing-yun, WANG Ning, JU Cheng-mei, WANG Jia, CAI Dun-jiang, SUN Guang-yu. Effects of Intercropping between Mulberry and Alfalfa on Soil Enzyme Activities and Microbial Community Diversity in Rhizophere[J]. Acta Agrestia Sinica, 2015, 23(2): 302-309.

张萌萌, 敖红, 李鑫, 张景云, 王宁, 鞠成梅, 王佳, 蔡敦江, 孙广玉. 桑树/苜蓿间作对根际土壤酶活性和微生物群落多样性的影响[J]. 草地学报, 2015, 23(2): 302-309.

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References

[1] 钦佩,安树青,颜京松. 生态工程学[M]. 南京:南京大学出版社,1998:1-3
[2] 张明如,翟明普,尹晶君,等. 农林复合生态系统的生态学原理及生态经济功能研究进展[J]. 中国水土保持科学,2003,1(4):66-77
[3] 周玉新,吴晶晶,唐罗忠. 林农复合系统的可持续发展研究[J]. 林业经济,2013,7:25
[4] 王凤娟,邓仰勇. 夏玉米与几种作物的间作技术[J]. 中国种业,2003,9:43
[5] 凤桂夙,肖靖秀,郑毅,等. 小麦蚕豆间作条件下蚕豆对钾的吸收及对蚕豆赤斑病的影响[J]. 云南农业大学学报,2005,20(6):779-782
[6] 范桂萍. 油菜/蚕豆间作控制病虫害研究[J]. 云南农业科技,2006(6):9-12
[7] 焦念元,宁堂原,赵春,等. 玉米间作复合体系光合特性的研究[J]. 作物学报,2006,32(6):917-923
[8] 吴凤芝,周新刚. 不同间作对黄瓜病害及土壤微生物群落多样性的影响[J]. 土壤学报,2009,46(5):899-906
[9] Nair P K R. State-of-the-art of agroforestry systems [J]. Forest Ecology and Management,1991,45(1):5-29
[10] 许楠,张会慧,朱文旭,等. 氮素形态对饲料桑树幼苗生长和光合特性的影响[J]. 草业科学,2012,29(10),1574-1580
[11] 郑晓媛,赵莉,许楠,等. 桑树大豆间作地上部和地下部的种间作用研究[J]. 土壤,2011,43(3):493-497
[12] 吴旭东,张晓娟,谢应忠,等. 不同种植年限紫花苜蓿人工草地土壤有机碳及土壤酶活性垂直分布特征[J]. 草业学报,2013,22(1):245-251
[13] 胡举伟,朱文旭,张会慧,等. 桑树/苜蓿间作对其生长及土地和光资源利用能力的影响[J]. 草地学报,2013,21(3):494-500
[14] 林先贵. 土壤微生物研究原理与方法[M]. 北京:高等教育出版社,2010:243-262
[15] 华孟,王坚. 土壤物理学[M]. 北京:北京农业大学出版社,1993:256-268
[16] Classen A T, Boyle S I, Haskins K E, et al. Community-level physiological profiles of bacteria and fungi: Plate type and incubation temperature influences on contrasting soils [J]. FEMS Microbiology Ecology,2003,44(3):319-328
[17] 董立国,蒋齐,蔡进军,等. 基于Biolog-ECO技术不同退耕年限苜蓿地土壤微生物功能多样性分析[J]. 干旱区研究,2011,28(4):630-637
[18] Salam A K, Katayama A, Kimura M. Activities of some soil enzymes in different land use systems after deforestation in hilly areas of West Lampung, South Sumatra, Indonesia [J]. Soil science and plant nutrition,1998,44(1):93-103
[19] Badiane N N Y, Chotte J L, Pate E, et al. Use of soil enzyme activities to monitor soil quality in natural and improved fallows in semi-arid tropical regions [J]. Applied soil ecology,2001,18(3):229-238
[20] Xiao Y, Li L, Zhang F. Effect of root contact on interspecific competition and N transfer between wheat and fababean using direct and indirect 15N techniques [J]. Plant and soil,2004,262(1/2):45-54
[21] 章铁,刘秀清,孙晓莉. 栗茶间作模式对土壤酶活性和土壤养分的影响[J]. 中国农学通报,2008,24(4):265-268
[22] Vandermeer J H. The ecology of intercropping [M]. Cambridge:Cambridge University Press,1992
[23] 贺明荣,冷寿慈. 桃粮间作对土壤养分状况及土壤生物活性的影响[J]. 土壤通报,1994,25(4):188-189
[24] 柴强,黄鹏,黄高宝. 间作对根际土壤微生物和酶活性的影响研究[J]. 草业学报,2006,14(5):105-110
[25] Zhang F, Li L. Using competitive and facilitative interactions in intercropping systems enhances crop productivity and nutrient-use efficiency [J]. Plant and soil,2003,248(1/2):305-312
[26] 唐劲驰, Mboreha I A,佘丽娜,等. 大豆根构型在玉米/大豆间作系统中的营养作用[J]. 中国农业科学,2005,38(6):1196-1203
[27] 刘均霞,陆引罡,远红伟,等. 玉米大豆间作条件下磷素的吸收利用[J]. 山地农业生物学报,2007,26(4):288-291
[28] 张恩和,黄高宝. 不同供磷水平下粮豆间套种植对根系分布和根际效应的影响[J]. 草业学报,1999,8(3):35-38
[29] 李隆,李晓林,张福锁,等. 小麦大豆间作条件下作物养分吸收利用对间作优势的贡献[J]. 植物营养与肥料学报,2000,6(2):140
[30] 许光辉,郑洪元,张德生,等. 长白山北坡自然保护区森林土壤微生物生态分布及其生化特性的研究[J]. 生态学报,1984,4(3):207-223
[31] Garland J L. Analysis and interpretation of community-level physiological profiles in microbial ecology [J]. FEMS Microbiology Ecology,1997,24(4):289-300
[32] 宋亚娜,张福锁,包兴国,等. 小麦/蚕豆, 玉米/蚕豆和小麦/玉米间作对根际细菌群落结构的影响[J]. 生态学报,2006,26(7):2268-2274
[33] 郝艳茹,劳秀荣,孙伟红,等. 小麦/玉米间作作物根系与根际微环境的交互作用[J]. 农村生态环境,2003,19(4):18-22
[34] 朱丽霞,章家恩,刘文高. 根系分泌物与根际微生物相互作用研究综述[J]. 生态环境,2003,12(1):102-105
[35] 李传涵,王长荣. 毛白杨、刺槐混交林丰产机理的研究[J]. 湖北林业科技,1991(1):1-5
[36] Li S M, Li L, Zhang F S, et al. Acid phosphatase role in chickpea/maize intercropping [J]. Annals of Botany,2004,94(2),297-303
[37] 王强,戴九兰,吴大千,等. 微生物生态研究中基于 BIOLOG 方法的数据分析[J]. 生态学报,2010,30(3):817-823
[38] 徐强,刘艳君,陶鸿. 间套作玉米对线辣椒根际土壤微生物生态特征的影响[J]. 中国生态农业学报,2013,21(9):1078-1087

Effects of Intercropping between Mulberry and Alfalfa on Soil Enzyme Activities and Microbial Community Diversity in Rhizophere

桑树/苜蓿间作对根际土壤酶活性和微生物群落多样性的影响

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