Identification and Evaluation of Wild Poa pratensis Varieties from Gansu for Drought Resistance During Germination Stage

doi:10.11733/j.issn.1007-0435.2016.05.017

Acta Agrestia Sinica ›› 2016, Vol. 24 ›› Issue (5): 1041-1049.DOI: 10.11733/j.issn.1007-0435.2016.05.017

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Identification and Evaluation of Wild Poa pratensis Varieties from Gansu for Drought Resistance During Germination Stage

NIU Kui-ju¹, JIN Xiao-yu¹, LI Hui-ping¹, XU Hai-peng¹, JIN Ning², MA Hui-ling¹

1. Pratacultural College, Gansu Agricultural University/Key laboratory of Grassland Ecosystem/Ministry of Education/Sino-U. S. Centers for Grazingland Ecosystem Sustainability, Lanzhou 730070, China;
2. Horticulture College, Gansu Agricultural University, Lanzhou 730070, China

Received:2015-10-12 Revised:2016-03-04 Online:2016-10-15 Published:2017-01-13

甘肃野生草地早熟禾萌发期抗旱性鉴定与评价

牛奎举¹, 金小煜¹, 李慧萍¹, 徐海鹏¹, 金宁², 马晖玲¹

1. 甘肃农业大学草业学院草业生态系统教育部重点实验室中-美草地畜牧业可持续发展研究中心, 甘肃兰州 730070;
2. 甘肃农业大学园艺学院, 甘肃兰州 730070

通讯作者: 马晖玲
作者简介:牛奎举(1991-),男,甘肃通渭人,博士,研究方向为牧草、草坪草种质资源及育种,E-mail:niukuiju@163.com
基金资助:
国家自然科学基金（31160482）资助

Abstract

Abstract:

Different concentrations of PEG-6000 (-0.1, -0.3, -0.5 and-0.7 MPa) was used to simulate drought stress environment. Germination rate, germination potential, vigor index and other four identification indices of 8 wild Poa pratensis varieties and 2 cultivars were measured under drought stress during germination stage. According to the relative value of seven identification indices as evaluation indices, drought resistance of Poa pratensis varieties was comprehensively evaluated and classified by the methods of membership function and cluster analysis. Furthermore, the role of each identification indices in evaluating drought resistance of different varieties was compared. The results showed that -0.1~-0.3MPa PEG-6000 was the optimum osmotic stress concentration to study drought resistance of Poa pratensis at germination stage. Seed vigor index and germination rate were more susceptible to drought resistance and closer to the comprehensive evaluation value of drought resistance, and it could be used as the most important indices for identification of drought resistance of Poa pratensis. The drought resistance of all varieties decreased in the order as:Qinzhou, Xihe, Longxi, Anding,Weiyuan, Tianzhu, Yuzhong, MidnightⅡ, Qingshui and Baron. The cluster analysis showed that Qingshui and Baron were sensitive to drought stress; three varieties had medium drought tolerance, for example Anding,Weyuan and MidnightⅡ; Qinzhou, Xihe and Longxi were the highest drought tolerant, which can be used to breeding new drought tolerant cultivars in the future.

Key words: Wild Poa pratensis, Germination stage, Drought resistance, Membership function, Cluster analysis

摘要：

采用-0.1，-0.3，-0.5和-0.7 MPa的聚乙二醇6000（PEG-6000）模拟不同程度的干旱胁迫，在萌发期分别处理8份野生草地早熟禾（Poa pratensis）种质和2个商用品种21 d，并测定发芽率、发芽势和活力指数等7个鉴定指标，并以其相对值作为评价指标，利用隶属函数法和聚类分析对供试的10份种质材料进行了综合评价和分类，同时，对各鉴定指标在草地早熟禾萌发期抗旱性评价中的作用进行了比较分析。结果表明，用于草地早熟禾萌发期抗旱性研究的适宜PEG-6000浓度为-0.1～-0.3 MPa，种子活力指数和发芽率受干旱胁迫的影响较大，且与抗旱性的关系最为密切，可作为草地早熟禾种质萌发期抗旱性鉴定的重要指标。10份种质材料抗旱性由强到弱依次为：秦州 > 西和 > 陇西 > 安定 > 渭源 > 天祝 > 榆中 > MidnightⅡ > 清水 > Baron；聚类分析可把供试材料按抗旱性强弱分为三类，其中清水和Baron属于干旱敏感种质；安定、渭源和MidnightⅡ等属于中等抗旱种质；秦州、西和和陇西属于抗旱种质，可作为抗旱育种材料。

关键词: 野生草地早熟禾, 萌发期, 抗旱性, 隶属函数, 聚类分析

CLC Number:

S543.9

NIU Kui-ju, JIN Xiao-yu, LI Hui-ping, XU Hai-peng, JIN Ning, MA Hui-ling. Identification and Evaluation of Wild Poa pratensis Varieties from Gansu for Drought Resistance During Germination Stage[J]. Acta Agrestia Sinica, 2016, 24(5): 1041-1049.

牛奎举, 金小煜, 李慧萍, 徐海鹏, 金宁, 马晖玲. 甘肃野生草地早熟禾萌发期抗旱性鉴定与评价[J]. 草地学报, 2016, 24(5): 1041-1049.

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Identification and Evaluation of Wild Poa pratensis Varieties from Gansu for Drought Resistance During Germination Stage

甘肃野生草地早熟禾萌发期抗旱性鉴定与评价

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