ISSR Analysis of Genetic Diversity of Introduced Red Clover Germplasm Resource

doi:10.11733/j.issn.1007-0435.2017.01.022

Acta Agrestia Sinica ›› 2017, Vol. 25 ›› Issue (1): 148-154.DOI: 10.11733/j.issn.1007-0435.2017.01.022

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ISSR Analysis of Genetic Diversity of Introduced Red Clover Germplasm Resource

CHAI Ji-kuan, ZHAO Gui-qin,MENG Li-juan

Pratacultural College, Gansu Agricultural University/Key Laboratory of Grassland Ecosystem/Ministry of Education/Sino-U.S. Centers for Grazingland Ecosystem Sustainability, Lanzhou, Gansu Province 730070, China

Received:2015-12-18 Revised:2016-12-26 Online:2017-02-15 Published:2017-05-06

引进红三叶种质资源遗传多样性的ISSR分析

柴继宽, 赵桂琴, 孟丽娟

甘肃农业大学草业学院草业生态系统教育部重点实验室中-美草地畜牧业可持续发展研究中心, 甘肃兰州 730070

通讯作者: 赵桂琴,E-mail:zhaogq@gsau.edu.cn
作者简介:柴继宽(1983-),男,甘肃陇西人,博士,讲师,主要研究方向为草种质资源及育种,E-mail:cjk_100@163.com
基金资助:
甘肃高校基本科研业务费项目（2012009）；农业部牧草种质资源保护项目（2013014）资助

Abstract

Abstract:

In order to analyze the genetic diversity of 31 red clover (Trifolium pratense), germplasm resources from Russia, Canada and America, provide reference information for their further utilization, ISSR-PCR molecular markers were used and tested in this paper. Five primers showed strong polymorphism and good repeatability, and were selected from 20 ISSR primers. The detected 61 bands including 58 polymorphic bands, the proportion of polymorphic bands (PPB) was 95.08%. The results from POPGENE analyses indicated that the genetic similarity coefficient of red clover germplasm ranged from 0.5902 to 0.9344, the average information index (I) of Shannon was 0.3427, genetic diversity index (H) of Nei was 0.2092 and Effective number of alleles(Ne) was 1.3162, indicating a comparatively rich genetic diversity among the tested materials. UPGMA cluster analyses gave a genetic similarity coefficient of 0.806 for boundaries and 31 materials were divided into five groups, Kenway from Canada had the highest genetic differentiation and clustered an unique group. This paper also compared clustering results of ISSR markers with those based on morphological characteristics.

Key words: Red clover, ISSR molecular marker, Germplasm resource, Genetic diversity

摘要：

为了分析从俄罗斯、美国和加拿大引进的31份红三叶（Trifolium pratense）种质资源的遗传多样性，为其进一步利用提供参考，本文采用ISSR分子标记技术对其进行了遗传多样性研究。从20条ISSR引物中筛选出多态性明显、重复性好的5条引物进行扩增，共扩增出61条谱带，其中多态性条带58条，多态性比率（PPB）高达95.08%。POPGENE分析结果表明，红三叶种质间遗传相似系数（GS）变化范围在0.5902~0.9344之间，平均Shannon信息指数（I）为0.3427，平均Nei's基因多样性指数（H）为0.2092，平均有效等位基因数（Ne）1.3162，表现出丰富的遗传多样性。利用UPGMA聚类分析，可将31份红三叶种质分为5大类，其中来自加拿大的24 号种质材料与其他材料间遗传分化最大，单独聚为一类。本文还比较了ISSR标记的聚类结果与基于形态特征的聚类结果之间的异同。

关键词: 红三叶, ISSR分子标记, 种质资源, 遗传多样性

CLC Number:

S541.2

CHAI Ji-kuan, ZHAO Gui-qin,MENG Li-juan. ISSR Analysis of Genetic Diversity of Introduced Red Clover Germplasm Resource[J]. Acta Agrestia Sinica, 2017, 25(1): 148-154.

柴继宽, 赵桂琴, 孟丽娟. 引进红三叶种质资源遗传多样性的ISSR分析[J]. 草地学报, 2017, 25(1): 148-154.

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ISSR Analysis of Genetic Diversity of Introduced Red Clover Germplasm Resource

引进红三叶种质资源遗传多样性的ISSR分析

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