Soil Microbial Diversity in Surface Crust and Non-Crust of Artificial Grassland in Black Soil Region of the Yellow River Source Region

doi:10.11733/j.issn.1007-0435.2018.01.005

Acta Agrestia Sinica ›› 2018, Vol. 26 ›› Issue (1): 45-52.DOI: 10.11733/j.issn.1007-0435.2018.01.005

Soil Microbial Diversity in Surface Crust and Non-Crust of Artificial Grassland in Black Soil Region of the Yellow River Source Region

LI Fa-xiang¹, ZHANG Tao², LUO Yu-zhu³, ZHANG Rui⁴, HAN Jin², BAI Yan-fu², DONG Quan-min⁵, ZHOU Hua-kun⁶, SHANG Zhan-huan²

1. College of Environmental Protection and Construction, Sanjiangyuan, Qinghai Provience 810008, China;
2. State Key Laboratory of Agroecological Ecosystems, College of Life Sciences, Lanzhou University, Lanzhou, Gansu Provience 730000, China;
3. Animal husbandry and veterinary workstation, Guoluo, Qinghai Provience 814000, China;
4. Northwest Institute of Eco-Environmental Resources, Chinese Academy of Sciences, Lanzhou, Gansu Provience 730020, China;
5. College of Animal Science and Veterinary Medicine, Qinghai University, Xining, Qinghai Provience 810000, China;
6. Northwest Institute of Plateau Biology, Chinese Academy of Science, Xining, Qinghai Provience 810008, China

Received:2017-06-06 Revised:2018-03-07 Online:2018-02-15 Published:2018-05-25

黄河源区黑土滩人工草地地表结皮与未结皮区土壤微生物多样性

李发祥¹, 张涛², 罗玉珠³, 张蕊⁴, 韩瑾², 白彦福², 董全民⁵, 周华坤⁶, 尚占环²

1. 青海三江源生态保护和建设办公室, 青海西宁, 810008;
2. 兰州大学生命科学院, 草地农业生态系统国家重点实验室, 甘肃兰州 730000;
3. 青海省果洛州畜牧兽医工作站, 青海果洛 814000;
4. 中国科学院西北生态环境资源研究院, 甘肃兰州 730020;
5. 青海大学畜牧兽医科学院, 青海西宁 81000;
6. 中国科学院西北高原生物研究所, 青海西宁 810008

通讯作者: 张涛
作者简介:李发祥(1963-),男,青海西宁人,本科,高级工程师,E-mail:qhlifx@163.com
基金资助:
国家重点研发计划课题（2016YFC0501906）；国家自然科学基金（41671508）；青海省创新平台建设专项（2017-ZJ-Y20）资助

Abstract

Abstract:

In this study, the microbial diversity of the crust and non-crust soil was examined in the artificial grassland. The results showed that dominant microbial community in the crust and non-crust soil was similar, belonging to the same phylum. The percentage of Proteobacteria in the crust soil was higher than non-crust soil. While the percentage of Actinobacteria was comparatively lower in the crust soil. PCA (Principal Component Analysis) demonstrated that the bacterial genetic distance was relatively minimum in the same sampling sites for crust as well as non-crust soil. This study suggests that the formation of soil biological crusts was closely associated with the change of the soil microbial community structure. So change in microbial community structure to a certain extent may develop soil biological crust.

Key words: Degraded grassland, Artificial grass, Soil biological crusts, Microbes

摘要：

为研究微生物群落多样性对土壤生物结皮的响应，本试验通过对有生物结皮和无生物结皮土壤微生物多样性的研究，分析黑土滩人工草地有生物结皮和无生物结皮土壤微生物细菌群落多样性。结果显示，无论是否有土壤生物结皮，土壤微生物细菌群落门水平的优势群落相同。变形菌门Proteobacteria群落都表现出在有生物结皮土壤中的百分含量大于五生物结皮土壤。放线菌门Actinobacteria和酸杆菌门Acidobacteria微生物含量在无生物结皮土壤中高于有生物结皮土壤。PCA分析结果表明，在相同采样地点有土壤生物结皮与无土壤生物结皮群落之间微生物细菌群落遗传距离较近，而不同采样点之间的微生物细菌群落遗传距离相对较远。本研究认为土壤生物结皮的形成与土壤中微生物群落结构的变化密切相关，微生物群落结构组成的变化在一定程度上能够说明土壤生物结皮的发生。

关键词: 退化草地, 人工草地, 土壤生物结皮, 微生物

CLC Number:

S154

LI Fa-xiang, ZHANG Tao, LUO Yu-zhu, ZHANG Rui, HAN Jin, BAI Yan-fu, DONG Quan-min, ZHOU Hua-kun, SHANG Zhan-huan. Soil Microbial Diversity in Surface Crust and Non-Crust of Artificial Grassland in Black Soil Region of the Yellow River Source Region[J]. Acta Agrestia Sinica, 2018, 26(1): 45-52.

李发祥, 张涛, 罗玉珠, 张蕊, 韩瑾, 白彦福, 董全民, 周华坤, 尚占环. 黄河源区黑土滩人工草地地表结皮与未结皮区土壤微生物多样性[J]. 草地学报, 2018, 26(1): 45-52.

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Soil Microbial Diversity in Surface Crust and Non-Crust of Artificial Grassland in Black Soil Region of the Yellow River Source Region

黄河源区黑土滩人工草地地表结皮与未结皮区土壤微生物多样性

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