PreliminaryAnalysis of Alfalfa Leaf Proteome under Salt-alkali Stress

doi:10.11733/j.issn.1007-0435.2019.03.008

Acta Agrestia Sinica ›› 2019, Vol. 27 ›› Issue (3): 574-580.DOI: 10.11733/j.issn.1007-0435.2019.03.008

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PreliminaryAnalysis of Alfalfa Leaf Proteome under Salt-alkali Stress

LI Bo¹, FANG Zhi-jian¹, WU Ting-ting¹, LI Hong², YANG Zhao², ZHAO Yu-jia¹

1. College of Agriculture, Forestry and Life Sciences, Qiqihar University, Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar, Heilongjiang Province 161006, China;
2. Institute of Animal Husbandry and Veterinary of Heilongjiang academy of Agricultural sciences, Qiqihar, Heilongjiang Province 161005, China

Received:2018-12-07 Revised:2019-04-13 Online:2019-06-15 Published:2019-07-19

盐碱胁迫下紫花苜蓿叶片蛋白组的初步分析

李波¹, 方志坚¹, 邬婷婷¹, 李红², 杨曌², 赵宇佳¹

1. 齐齐哈尔大学生命科学与农林学院, 抗性基因工程与寒地生物多样性保护黑龙江省重点实验室, 黑龙江齐齐哈尔 161006;
2. 黑龙江省农业科学院畜牧兽医分院, 黑龙江齐齐哈尔 161005

作者简介:李波(1962-),女,辽宁鞍山人,教授,硕士生导师,主要从事细胞生物学研究,E-mail:libo1962@163.com
基金资助:
黑龙江省省属高等学校基本科研业务费科研项目（135209267，YSTSXK201886）；黑龙江省应用技术研究与开发计划重大项目（GA15B105-5）资助

Abstract

Abstract: The alfalfa (Medicago sativa) variety "WL343HQ" was used as the material,and the seedlings were treated with the mixture of 150 mmol/L Na₂CO₃ and NaHCO₃. The changes of protein expression in leaves under salt-alkali stress were analyzed using iTRAQ combined with reversed-phase liquid chromatography and liquid chromatography-tandem chromatography,and the differential proteins were analyzed by bioinformatics. The results showed that 318 significant differential proteins were identified under salt-alkali stress treatment,including 172 up-regulated proteins and 146 down-regulated proteins. The function of these differential proteins involved a variety of metabolic pathways,in which the amount of protein expression associated with photosynthesis was generally down-regulated,and the protein expression levels associated with phenylpropanoid biosynthesis,phenylalanine metabolism,and flavonoid biosynthesis were generally up-regulated. Proteomic analysis technology could effectively screen differentially expressed proteins in alfalfa leaves,which could provide a theoretical basis for further study on the molecular mechanism of alfalfa to deal with salt-alkali stress.

Key words: Medicago sativa L., Soda salt-alkali stress, Proteome, Differential protein, Metabolic pathway

摘要： 以紫花苜蓿（Medicago sativa）品种"WL343HQ"为材料，对其幼苗进行150 mmol·L^-1的Na₂CO₃和NaHCO₃混合盐碱胁迫，采用iTRAQ技术结合反相液相色谱与液相串联色谱，分析盐碱胁迫叶片中蛋白表达的变化，并对获得的差异蛋白进行生物信息学分析。结果表明，在盐碱胁迫处理下共鉴定到318个显著差异蛋白，包括172个上调蛋白和146个下调蛋白，这些差异蛋白的功能涉及多种代谢途径，其中与光合作用相关的蛋白质表达量总体下调，与苯丙素生物合成、苯丙氨酸代谢和类黄酮生物合成相关的蛋白质表达量总体上调。通过蛋白组学分析技术可有效筛选紫花苜蓿叶片中差异表达蛋白，可为深入研究紫花苜蓿应对盐碱胁迫的分子机制提供一定的理论基础。

关键词: 紫花苜蓿, 苏打盐碱胁迫, 蛋白质组, 差异蛋白, 代谢途径

CLC Number:

S963.22+3.3

LI Bo, FANG Zhi-jian, WU Ting-ting, LI Hong, YANG Zhao, ZHAO Yu-jia. PreliminaryAnalysis of Alfalfa Leaf Proteome under Salt-alkali Stress[J]. Acta Agrestia Sinica, 2019, 27(3): 574-580.

李波, 方志坚, 邬婷婷, 李红, 杨曌, 赵宇佳. 盐碱胁迫下紫花苜蓿叶片蛋白组的初步分析[J]. 草地学报, 2019, 27(3): 574-580.

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PreliminaryAnalysis of Alfalfa Leaf Proteome under Salt-alkali Stress

盐碱胁迫下紫花苜蓿叶片蛋白组的初步分析

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