Effects of Exogenous Putrescine and Spermidine on Cold Tolerance of Centipede Grass

doi:10.11733/j.issn.1007-0435.2019.03.012

Acta Agrestia Sinica ›› 2019, Vol. 27 ›› Issue (3): 603-611.DOI: 10.11733/j.issn.1007-0435.2019.03.012

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Effects of Exogenous Putrescine and Spermidine on Cold Tolerance of Centipede Grass

LIU Nan-qing^1,2, LIN Shao-yan³, SHEN Yi-xin¹

1. College of Agro-Grassland Science, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China;
2. Department of Landscape Architecture, Jiangsu Polytechnic College of Agriculture and Forestry, Jurong, Jiangsu Province 212400, China;
3. State Key Laboratory of Crop Genetics and Enhancement, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China

Received:2018-12-05 Revised:2019-04-13 Online:2019-06-15 Published:2019-07-19

外源腐胺与亚精胺提高假俭草低温胁迫耐受性的研究

刘南清^1,2, 林绍艳³, 沈益新¹

1. 南京农业大学草业学院, 江苏南京 210095;
2. 江苏农林职业技术学院, 江苏句容 212400;
3. 南京农业大学作物遗传与种质创新国家重点实验室, 江苏南京 210095

通讯作者: 沈益新
作者简介:刘南清(1979-),男,广西平南人,博士研究生,副教授,主要从事草坪草逆境生理研究,E-mail:Liunanqing@jsafc.edu.cn
基金资助:
高温高湿区牧草栽培加工和利用研究项目（2017YFD0502106-1）资助

Abstract

Abstract: Effect of foliar application exogenous putrescine (Put) and spermidine (Spd) on the antioxidant enzyme activities and osmolyte contents of centipede grass[Eremochloa ophiuroides (Munro.) Hacks] under low temperature stress was researched in this study. The results showed that the application of exogenous Put and Spd at a concentration of 0.1mM could significantly decrease the leaf electrolyte leakage (EL) and malondialdehyde (MDA) content,while significantly increase the antioxidant enzyme activities including super oxidase dismutase (SOD),peroxidase (POD),asorbate peroxidase (APX) and catalase (CAT),the osmolyte contents such as soluble sugar (SS) and proline (Pro),and Put,Spd and spermine (Spm) contents,during the low temperature stress. These indicated that the application of exogenous polyamines could increase the antioxidant enzyme activities as well as the osmolyte contents,and decrease the stress damage of the centipede grass during low temperature stress. The results provided valuable references for improving cold resistance of centipede grass.

Key words: Centipede grass, Exogenous polyamines, Low temperature stress, Antioxidant enzyme activities, Osmolytes

摘要： 为明确外源多胺对低温胁迫下假俭草体内的抗氧化酶活性及渗透调节物质含量的调节作用，以及对假俭草耐寒性的影响，本文测定分析了外源多胺处理对低温胁迫下假俭草叶片组织的相对电导率、丙二醛含量、抗氧化酶活性以及渗透调节物质含量的影响。研究结果表明：喷施0.1mM浓度的外源腐胺与亚精胺显著降低假俭草在低温胁迫过程中叶片组织的相对电导率与丙二醛含量，并显著提高了超氧化物歧化酶、过氧化物酶、抗坏血酸过氧化物酶与过氧化氢酶等抗氧化酶的活性，以及可溶性多糖、脯氨酸、腐胺、亚精胺与精胺等渗透调节物质的含量。研究结果证实了外源多胺处理可以通过提高假俭草叶片组织抗氧化酶的活性和渗透调节物质的含量，减轻组织在低温胁迫下的伤害程度，这为草坪养护过程中使用外源多胺物质提高假俭草耐寒性提供了依据。

关键词: 假俭草, 外源多胺, 低温胁迫, 抗氧化酶活性, 渗透调节物质

CLC Number:

Q945

LIU Nan-qing, LIN Shao-yan, SHEN Yi-xin. Effects of Exogenous Putrescine and Spermidine on Cold Tolerance of Centipede Grass[J]. Acta Agrestia Sinica, 2019, 27(3): 603-611.

刘南清, 林绍艳, 沈益新. 外源腐胺与亚精胺提高假俭草低温胁迫耐受性的研究[J]. 草地学报, 2019, 27(3): 603-611.

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Effects of Exogenous Putrescine and Spermidine on Cold Tolerance of Centipede Grass

外源腐胺与亚精胺提高假俭草低温胁迫耐受性的研究

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