Acta Agrestia Sinica

Acta Agrestia Sinica ›› 2020, Vol. 28 ›› Issue (3): 589-596.DOI: 10.11733/j.issn.1007-0435.2020.03.001

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Effects of Exogenous Proline on Growth and Antioxidant Enzyme Related Gene Expression of Elymus nutans under Low Temperature Stress

PUBU Zhuo-ma1,2, QIMEI La-mu1, LI Dan-dan1, DUOJI Dun-zhu2, ZHANG Jing1, SUN Yun-fu1, MIAO Yan-jun3, FU Juan-juan1, HU Tian-ming1   

  1. 1. Department of Grassland Science, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi Province 712100, China;
    2. Tibet Academy of Agriculture and Animal Husbandry Sciences, Lasa, Tibet 850000, China;
    3. College of Plant Science, Agricultural and Animal Husbandry College of Tibet University, Linzhi, Tibet 860000, China
  • Received:2019-12-09 Revised:2020-02-16 Online:2020-06-15 Published:2020-05-30

外源脯氨酸对低温胁迫下西藏野生垂穗披碱草幼苗生长和抗氧化酶相关基因表达的影响

普布卓玛1,2, 其美拉姆1, 李丹丹1, 多吉顿珠2, 张靖1, 孙运府1, 苗彦军3, 付娟娟1, 呼天明1   

  1. 1. 西北农林科技大学草业与草原学院, 陕西 杨凌 712100;
    2. 西藏自治区农科院草业科学研究所, 西藏 拉萨 850000;
    3. 西藏农牧学院植物科学学院, 西藏 林芝 860000
  • 通讯作者: 付娟娟, 呼天明
  • 作者简介:普布卓玛(1991-),女,藏族,西藏拉萨人,硕士研究生,主要从事植物逆境生物学研究,E-mail:puzhuo050509@nwafu.edu.cn;
  • 基金资助:
    国家自然科学基金项目（31901382）；西北农林科技大学科研启动经费项目（2452017195）；中国博士后科学基金项目（2018M631208）；国家自然科学基金项目（31872411）；国家牧草产业技术体系拉萨综合试验站项目（CARS-34）共同资助

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Abstract: The wild Elymus nutans in Tibet was used as a test material,and 10 mM proline was sprayed on the leaves of E. nutans to investigate the physiological responses and the expression of antioxidant enzyme-related genes of plants in response to cold stress (4℃). The results showed that proline treatment significantly increased the root length (27.11%),aboveground fresh (48.80%) and dry weight (48.83%),and underground fresh and dry weight (51.74% and 38.87%) of E. nutans under low temperature stress (4℃). Meanwhile,pretreatment with proline increased proline and soluble sugar contents by 42.96% and 22.98% (P<0.05),respectively;increased the relative expression level of APX,CAT,ChICu/ZnSOD, and CytCu/ZnSOD genes. Antioxidant enzymes activities of POD,CAT,SOD,and APX were also improved (P<0.05) by proline treatment. Proline treatment led to a decrease in MDA accumulation and relative conductivity by 47.84% and 20.87%,respectively (P<0.05). In summary,these findings suggest that proline regulates the stability of cell membrane structure through enhancing the ability of osmotic adjustment and antioxidant defense activities,thereby improves the cold tolerance of E. nutans.

Key words: Proline, Low temperature stress, Elymus nutans, Antioxidant enzyme, Gene expression

摘要： 本试验以西藏野生垂穗披碱草（Elymus nutans Griseb.）为研究对象，采用盆栽试验，研究叶面喷施外源脯氨酸（10 mM）对低温胁迫（4℃）下垂穗披碱草幼苗生长及抗氧化酶活性及其基因表达的影响。结果表明：低温胁迫下脯氨酸处理能够显著提高垂穗披碱草幼苗的根长、地上和地下鲜重、地上和地下干重，与低温胁迫相比分别增加27.11%，48.80%，48.83%，51.74%，38.87%；外源脯氨酸增加了低温胁迫下内源脯氨酸和可溶性糖的积累，分别增加42.96%和22.98%（P<0.05）；而且脯氨酸处理提高了APX、CAT、ChICu/ZnSOD、CytCu/ZnSOD基因的表达水平以及过氧化物酶（Peroxidase，POD）、过氧化氢酶（Catalase，CAT）、超氧化物歧化酶（Superoxide dismutase，SOD）和抗坏血酸过氧化物酶（Ascorbate peroxidase，APX）活性，降低了丙二醛（Malondialdehyde，MDA）含量和相对电导率水平（P<0.05）。综上所述，脯氨酸能够增强低温胁迫下垂穗披碱草幼苗的渗透调节能力及抗氧化酶活性，维持细胞膜的稳定性，缓解其生长抑制，从而提高垂穗披碱草的抗寒性。

关键词: 脯氨酸, 低温胁迫, 垂穗披碱草, 抗氧化酶, 基因表达

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