Effects of UV-B Radiation on the Growth and Antioxidative Enzymes System of Two Eco-types Elymus nutans Seedlings

doi:10.11733/j.issn.1007-0435.2019.04.014

Acta Agrestia Sinica ›› 2019, Vol. 27 ›› Issue (4): 898-905.DOI: 10.11733/j.issn.1007-0435.2019.04.014

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Effects of UV-B Radiation on the Growth and Antioxidative Enzymes System of Two Eco-types Elymus nutans Seedlings

LIU Qian¹, LIU Bo-wen¹, WANG Yang¹, AO Bao¹, TIAN Pei-xin¹, WEN Su-yun¹, ZHANG Xu-hu¹, MIAO Yan-jun², XU Yue-fei¹

1. College of Grassland Agriculture, Northwest A & F University, Yangling, Shaanxi Province 712100, China;
2. College of Plant Science, Tibet Agriculture and Animal Husbandry College, Linzhi, Tibet 860000, China

Received:2018-09-29 Revised:2019-03-22 Online:2019-08-15 Published:2019-09-26

UV-B辐射对不同生态型垂穗披碱草幼苗生长和抗氧化酶系统的影响

刘茜¹, 刘博文¹, 王洋¹, 奥宝¹, 田沛鑫¹, 文素芸¹, 张旭虎¹, 苗彦军², 许岳飞¹

1. 西北农林科技大学草业与草原学院, 陕西杨凌 712100;
2. 西藏农牧学院植物科学学院, 西藏林芝 860000

通讯作者: 许岳飞
作者简介:刘茜(1996-),女,汉族,陕西商洛人,硕士研究生,主要从事牧草、草坪草逆境生物学研究,E-mail:liuqian9577@nwsuaf.edu.cn
基金资助:
陕西省畜牧技术推广项目（20181214000004）；中央高校基本科研业务费专项资金资助（2452019080）资助

Abstract

Abstract: UV-B radiation is one of the limiting factors affecting plant growth. The effects of UV-B radiation on the growth and antioxidative enzymes system of two ecological types of Elymus nutans (Commercial type from Beijing zhengdao,ZD;Tibetan wild-type from Xainza,XZ) seedlings were studied using sand culture method. The results showed that the biomass and chlorophyll content of two eco-types of E. nutans were inhibited to varying degrees after UV-B radiation treatment,and the growth of ZD's below-ground biomass and root-shoot index was significantly inhibited. UV-B radiation treatment significantly increased the malondialdehyde content of E. nutans,and the malondialdehyde content of XZ was significantly lower than ZD. And,UV-B radiation significantly increased the soluble protein content of E. nutans, and lipoxygenase activity of ZD increased significantly,but there was no significant difference between the UV-B radiation treatment and the control in XZ. The superoxide dismutase and peroxidase activities of two eco-types E. nutans didn't not change significantly after UV-B radiation treatment. The activity of superoxide dismutase in XZ was significantly higher than that in ZD,while the activity of peroxidase was significantly lower than that in ZD. However,the activities of catalase and glutathione reductase were significantly affected by UV-B radiation,but there was no significant difference between the two eco-types. It is reasonably to infer that XZ is less affected by UV-B radiation than ZD and has higher resistance to UV-B radiation,which may be related to its stable lipoxygenase activity and higher superoxide dismutase activity.

Key words: UV-B radiation, Elymus nutans, Antioxidative enzymes system, Tibet, Ecological type

摘要： UV-B辐射是影响植物生长的外界限制因子之一。本文研究了UV-B辐射对2个生态型垂穗披碱草（Elymus nutans）（商用型正道，ZD；西藏野生型申扎，XZ）幼苗的生长和抗氧化系统的影响。结果表明：UV-B辐射处理后，二者的生物量和叶绿素含量受到不同程度地抑制，ZD地下生物量和根茎比受UV-B辐射抑制作用显著；处理后二者的丙二醛含量显著提高，且XZ的丙二醛含量显著低于ZD；UV-B还显著增加了垂穗披碱草幼苗的可溶性蛋白含量，ZD的脂氧合酶活性也有显著提高，但XZ并无显著变化；另外，幼苗的超氧化物歧化酶和过氧化物酶活性受UV-B辐射影响均不显著，但XZ的超氧化物歧化酶活性显著高于ZD，而过氧化物酶活性显著低于ZD；二者的过氧化氢酶和谷胱甘肽还原酶活性受UV-B辐射影响显著，但生态型间无显著差异。综上，XZ受UV-B辐射影响小于ZD，对UV-B辐射有较高抗性，这可能与其稳定的脂氧合酶活性和较高的超氧化物歧化酶活性等有关。

关键词: UV-B辐射, 垂穗披碱草, 抗氧化酶系统, 西藏, 生态型

CLC Number:

Q945.78

LIU Qian, LIU Bo-wen, WANG Yang, AO Bao, TIAN Pei-xin, WEN Su-yun, ZHANG Xu-hu, MIAO Yan-jun, XU Yue-fei. Effects of UV-B Radiation on the Growth and Antioxidative Enzymes System of Two Eco-types Elymus nutans Seedlings[J]. Acta Agrestia Sinica, 2019, 27(4): 898-905.

刘茜, 刘博文, 王洋, 奥宝, 田沛鑫, 文素芸, 张旭虎, 苗彦军, 许岳飞. UV-B辐射对不同生态型垂穗披碱草幼苗生长和抗氧化酶系统的影响[J]. 草地学报, 2019, 27(4): 898-905.

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References

[1] Norsang G,Kocbach L,Tsoja W,et al. Ground-based measurements and modeling of solar UV-B radiation in Lhasa,Tibet[J]. Atmospheric Environment,2009,43(8):1498-1502
[2] Norsang G,Ma L,Dahlback A,et al. The vitamin D status among Tibetans[J]. Photochemistry & Photobiology,2010,85(4):1028-1031
[3] 孙金伟,任斐鹏,任亮,等. UV-B辐射对植物生理生态特征的影响研究进展[J]. 长江科学院院报,2015,32(3):107-111
[4] 李永鑫. 西藏地表生物有效紫外辐射观测研究[D]. 拉萨:西藏大学,2015:3
[5] 卢宝荣. 披碱草属与大麦属系统关系的研究[J]. 植物分类学报,1997,35(3):193-207
[6] 陈智华,苗佳敏,钟金城,等. 野生垂穗披碱草种质遗传多样性的SRAP研究[J]. 草业学报,2009,18(5):192-200
[7] 马啸,周永红,于海清,等. 野生垂穗披碱草种质的醇溶蛋白遗传多样性分析[J]. 遗传,2006,28(6):699-706
[8] Zhou X,Li C,Zhang S. Effects of ambient ultraviolet-B on ecophysiological characteristics and forage quality of Elymus nutans in the Tibetan alpine grasslands[J]. Grassland Science,2014,60(2):98-103
[9] Yun H,Lim S,Kim Y X,et al. Diurnal Changes in C,N Metabolism and Response of Rice Seedlings to UV-B Radiation[J]. Journal of Plant Physiology,2018,228:66-74
[10] Zhang X,Ding X,Ji Y,et al. Measurement of metabolite variations and analysis of related gene expression in Chinese liquorice (Glycyrrhiza uralensis) plants under UV-B irradiation[J]. Scientific Reports,2018,8(1):6144
[11] Januškaitiene·I,Kaciene·G. The effect of foliar spray fertilizers on the tolerance of Hordeum vulgare to UV-B radiation and drought stress[J]. Cereal Research Communications,2017,45(3):1-11
[12] 侯常伟,白涛,王忆,等. UV-B辐射对光核桃光合作用和内源激素水平的影响[J]. 中国农学通报,2012,28(22):184-189
[13] Allen D J,Nogues S,Baker N R. Ozone depletion and increased UV-B radiation:is there a real threat to photosynthesis[J]. Journal of Experimental Botany,1998,49(328):1775-1788
[14] Fisher R A,Tanabe S,Buxton D B,et al. Ultraviolet-B irradiation-induced freezing tolerance in relation to antioxidant system in winter wheat (Triticum aestivum L.) leaves[J]. Environmental & Experimental Botany,2007,60(3):300-307
[15] 许大全. 叶绿素含量的测定及其应用中的几个问题[J]. 植物生理学报,2009,45(9):896-898
[16] Li Y,Zhao H,Duan B,et al. Effect of drought and ABA on growth,photosynthesis and antioxidant system of Cotinus coggygria,seedlings under two different light conditions[J]. Environmental & Experimental Botany,2011,71(1):107-113
[17] Elstner E F,Heupel A. Inhibition of nitrite formation from hydroxylammonium chloride:a simple assay for superoxide dismutase.[J]. Analytical Biochemistry,1976,70(2):616-620
[18] Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical Biochemistry,1976,72(s1-2):248-254
[19] 石胜尧,张延坤,郭大发,等. 大豆脂肪氧化酶活性的测定[J]. 营养学报,1996(3):354-357
[20] Beauchamp C,Fridovich I. Superoxide dismutase:Improved assays and an assay applicable to acrylamide gels[J]. Analytical Biochemistry,1971,44(1):276-287
[21] Cakmak I,Marschner H. Magnesium deficiency and high light intensity enhance activities of superoxide dismutase,ascorbate peroxidase,and glutathione reductase in bean leaves[J]. Plant Physiology,1992,98(4):1222-1227
[22] Upadhyaya A,Sankhla D,Davis T D,et al. Effect of paclobutrazol on the activities of some enzymes of activated oxygen metabolism and lipid peroxidation in senescing soybean leaves[J]. Journal of Plant Physiology,1985,21(5):453-461
[23] Foyer C H,Halliwell B. The presence of glutathione and glutathione reductase in chloroplasts:A proposed role in ascorbic acid metabolism[J]. Planta,1976,133(1):21-25
[24] Mazza C A,Giménez P I,Kantolic A G,et al. Beneficial effects of solar UV-B radiation on soybean yield mediated by reduced insect herbivory under field conditions[J]. Physiologia Plantarum,2013,147(3):307-315
[25] 张玉红,陈路瑶,刘彤,等. 增补UV-B辐射对药用植物黄檗幼苗生长及光合生理影响[J]. 中国农学通报,2018,34(4):76-82
[26] He J,Huang L K,Chou W S. Effects of supplementary ultraviolet-B radiation on rice and pea plants[J]. Functional Plant Biology,1993,20(2):129-142
[27] Ranjbarfordoei A,Samson R,Damme P V. Photosynthesis performance in sweet almond (Prunus dulcis (Mill) D. Webb) exposed to supplemental UV-B radiation[J]. Photosynthetica,2011,49(1):107-111
[28] 刘智微,钟小仙,沈益新. 海盐胁迫对苏牧2号象草抗氧化酶活性和MDA含量的影响[J]. 草地学报,2011,33(1):24-29
[29] 刘碧容,甄畅迪,萧洪东,等. 硼对草坪草超氧化物歧化酶活性,超氧阴离子产生速率和丙二醛含量的影响[J]. 华中农业大学学报,2008,7(3):378-381
[30] 刘鹏,杨玉爱. 钼,硼对大豆叶片膜脂过氧化及体内保护系统的影响[J]. 植物学报,2000,42(5):461-466
[31] Di V A,Salucci M L,Van Z G,et al. Structure-to-Function Relationship of Mini-Lipoxygenase,a 60-kDa Fragment of Soybean Lipoxygenase-1 with Lower Stability but Higher Enzymatic Activity[J]. Journal of Biological Chemistry,2003,278(20):18281-18288
[32] 胡廷章,胡宗利,屈霄霄,等. 植物脂肪氧化酶的研究进展[J]. 生物工程学报,2009,25(1):1-9
[33] Porta H,Ruedabenítez P,Campos F,et al. Analysis of Lipoxygenase mRNA Accumulation in the Common Bean (Phaseolus vulgaris L.) during Development and under Stress Conditions[J]. Plant & Cell Physiology,1999,40(8):850-858
[34] Mittler R,Vanderauwera S,Gollery M,et al. Reactive oxygen gene network of plants[J]. Trends in Plant Science,2004,9(10):490-498
[35] Kataria S,Jajoo A,Guruprasad K N. Impact of increasing Ultraviolet-B (UV-B) radiation on photosynthetic processes.[J]. Journal of Photochemistry & Photobiology Biology,2014,137(8):55-66
[36] 褚润,陈年来,韩国君,等. UV-B辐射增强对芦苇生长及生理特性的影响[J]. 环境科学学报,2018,38(05):389-396
[37] 吴永波,薛建辉. UV-B辐射增强对植物影响的研究进展[J]. 世界林业研究,2004,17(3):29-31

Effects of UV-B Radiation on the Growth and Antioxidative Enzymes System of Two Eco-types Elymus nutans Seedlings

UV-B辐射对不同生态型垂穗披碱草幼苗生长和抗氧化酶系统的影响

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