Acta Agrestia Sinica ›› 2019, Vol. 27 ›› Issue (6): 1781-1785.DOI: 10.11733/j.issn.1007-0435.2019.06.039

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Physiological Responses of Elymus nutans to Cu2+ and Zn2+ Stress

WANG Chuan-qi1,2, FU Juan-juan3, LIU Min-jie1, ZHOU Qing-ping4, MIAO Yan-jun2   

  1. 1. Qinghai Academy of Animal Science and Veterinary Medicine, Qinghai University, Xining, Qinghai Province 810016, China;
    2. Tibet Agriculture and Animal Husbandry University, Tibet, Nyingchi, 860000, China;
    3. Northwest A&F University, Yangling, Shanxi Province 712100, China;
    4. Southwest Minzu University, Chengdu, Sichuan Province 610041, China
  • Received:2019-09-19 Revised:2019-10-18 Online:2019-12-15 Published:2019-12-31

垂穗披碱草对Cu2+,Zn2+胁迫的生理响应

王传旗1,2, 付娟娟3, 刘敏洁1, 周青平4, 苗彦军2   

  1. 1. 青海大学畜牧兽医科学院, 青海 西宁 810016;
    2. 西藏农牧学院, 西藏 林芝 860000;
    3. 西北农林科技大学, 陕西 杨凌 712100;
    4. 西南民族大学, 四川 成都 610041
  • 通讯作者: 陈积山,E-mail:myj666@126.com
  • 作者简介:王传旗(1991-),男,河南武陟人,博士研究生,主要从事高原牧草遗传育种资源研究与利用研究,E-mail:18889046706@163.com
  • 基金资助:
    西藏自治区科技厅重点项目(XZ201901NA03);国家重点研发计划项目(2016YFC0502002;2018YFD0502401-1)资助

Abstract: The paper aims to explore the Physiological adaptation mechanism of wild Elymus nutans to Cu2+ and Zn2+ stress in Baqing County,Tibet. The experiment took wild Elymus nutans from Baqing County as materials,and studied the effects of different concentrations of Cu2+ and Zn2+ on seed germination and seedling growth by paper germination method. The results were as follows:300 mg·L-1 Cu2+ and 500 mg·L-1 Zn2+ treatment significantly inhibited seed germination and seedling growth of wild Elymus nutans in Baqing County,Tibet. Low concentration of Zn2+ (200 mg·L-1) promoted seed germination;Under low concentration of Cu2+(20 mg·L-1) and Zn2+(200 mg·L-1) stress,the root length of seeds was slightly longer than that of the control. With the increase of Cu2+ and Zn2+ concentration,the content of chlorophyll in leaves of Elymus nutans seedlings decreased,while the relative conductivity and Malondialdehyde content increased. It can be seen that wild Elymus nutans in Baqing County,Tibet,has a low tolerance for Cu2+ stress,but has certain tolerance to Zn2+ stress,and has strong application potential in vegetation restoration of Zn2+ contaminated soil in central Tibet.

Key words: Elymus nutans, Domestication, Cu2+, Zn2+, Cell membrane damage

摘要: 为探索西藏巴青县野生垂穗披碱草(Elymus nutans)对Cu2+,Zn2+胁迫的生理适应机理,本试验以巴青县地区的野生垂穗披碱草为材料,采用纸上发芽法,研究了不同浓度Cu2+和Zn2+胁迫对其种子萌发和幼苗生长的影响。结果表明:300 mg·L-1Cu2+和500 mg·L-1 Zn2+处理下,垂穗披碱草种子萌发及幼苗生长受到显著抑制(P<0.05);而低浓度的Zn2+(200 mg·L-1)处理促进了种子萌发;20 mg·L-1的Cu2+和200 mg·L-1Zn2+处理下,种子根长略高长于对照;随着Cu2+和Zn2+胁迫浓度的增大,幼苗叶片叶绿素含量表现为下降趋势,相对电导率和丙二醛含量呈现上升趋势。由此可知,垂穗披碱草对Cu2+的耐受性较差,但具有一定的耐Zn2+胁迫能力,在西藏中部Zn2+污染土壤的植被修复中具有较强的应用潜力。

关键词: 垂穗披碱草, 驯化, Cu2+, Zn2+, 细胞膜损伤

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