Acta Agrestia Sinica ›› 2020, Vol. 28 ›› Issue (2): 305-318.DOI: 10.11733/j.issn.1007-0435.2020.02.003

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Transcriptome Comparative Analysis of Different Qinghai Wild Poa pratensis Under Low Temperature Stress

ZHAO Chun-xu1, MA Xiang2, Dong Wen-ke1, Niu Kui-ju1, Zhang Ran1, MA Hui-ling1   

  1. 1. College of Pratacultural Science, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem, Ministry of Education, Pratacultural Engineering Laboratory of Gansu Province, Sino-U. S. Center for Grassland Ecosystem Sustainability, Lanzhou, Gansu Province 730070, China;
    2. Key Laboratory of Superior Forage Germplasm in the Qinghai-Tibetan Plateau, Qinghai Academy of Animal Science and Veterinary Medicine, Qinghai University, Xining, Qinghai Province 810016, China
  • Received:2019-12-22 Revised:2020-02-13 Online:2020-04-15 Published:2020-04-11

低温胁迫下不同青海野生草地早熟禾的转录组比较分析

赵春旭1, 马祥2, 董文科1, 牛奎举1, 张然1, 马晖玲1   

  1. 1. 甘肃农业大学草业学院, 草业生态系统教育部重点实验室, 甘肃省草业工程实验室, 中-美草地畜牧业可持续发展研究中心, 甘肃 兰州 730070;
    2. 青海省青藏高原优良牧草种质资源利用重点实验室, 青海省畜牧兽医科学院, 青海大学, 青海 西宁 810016
  • 通讯作者: 马晖玲
  • 作者简介:赵春旭(1985-),男,甘肃兰州人,博士研究生,主要从事牧草及草坪草种质资源研究,E-mail:zhaocx@gsau.edu.cn
  • 基金资助:
    青海省科技厅重点实验室发展专项“青海省青藏高原优良牧草种质资源利用重点实验室”(2020-ZJ-Y03)资助

Abstract: In order to explore the molecular response mechanism to low temperature stress of Qinghai wild Poa pratensis,this experiment took cold-resistant germplasm material ‘10-122’ and low-temperature sensitive germplasm material ‘09-126’ as research objects and analyzed transcriptome differences between low temperature stress and normal temperature controls with high-throughput Illumina Hiseq sequencing technology. The results showed that there were 31 943 differentially expressed genes detected in ‘10-122’,in which 17088 differentially expressed genes were up-regulated and 14855 differentially expressed genes were down-regulated. There were 25 905 differentially expressed genes detected in ‘09-126’,in which 13 513 differentially expressed genes were up-regulated and 12 392 differentially expressed genes were down-regulated. The enrichment analysis of differentially expressed genes on two germplasm materials showed that the differentially expressed genes were significantly enriched in photosynthesis,oxidation-reduction,carbohydrate metabolism,cell membrane system,transporter protein,and secondary metabolism under low temperature stress. Additionally,a number of DEGs involved in Ca2+ signaling,plant hormone biosynthesis and signaling transduction,antioxidant system and carbohydrate metabolism pathways were specific up-regulated in cold-resistant genotype ‘10-122’. Therefore,these DEGs including CMLCPKCALMDHARGSTNCEDSNRK2、BSKCKXBIN2、ARF were identified as potential genes associated with the cold resistance of ‘10-122’ genotype.

Key words: Low-temperature stress, wild Poa pratensis, Transcriptome, Differentially expressed genes

摘要: 为探究青海野生草地早熟禾(Poa pratensis)低温胁迫下的分子应答机制,本试验以青海省野生草地早熟禾耐寒材料‘10-122’和低温敏感材料‘09-126’为研究对象,采用高通量Illumina Hiseq测序技术分析了在低温胁迫与常温对照间的转录组差异。结果表明:‘10-122’共有31 943个差异表达基因,其中,17 088个差异表达基因上调表达,14 855个差异表达基因下调表达;‘09-126’共有25 905个差异表达基因,其中,13 513个差异表达基因上调表达,12 392个差异表达基因下调表达;对2种材料进行差异表达基因富集分析,得出差异表达基因低温胁迫下在光合作用、氧化还原反应过程、碳水化合物代谢、细胞膜系统、转运蛋白以及次生物代谢中富集显著;此外,一些钙信号调节、激素代谢和信号传导、抗氧化系统、碳水化合物代谢等通路的基因仅在耐寒型种质材料‘10-122’上调表达,可作为潜在的抗寒基因,如CMLCPKCALMDHARGSTNCEDSNRK2、BSKCKXBIN2、ARFPEK等。

关键词: 低温胁迫, 野生草地早熟禾, 转录组, 差异表达基因

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