Incidence of Oat Powdery Mildew and Pathogen Identification in the Main Oat Production Areas of Gansu Province

doi:10.11733/j.issn.1007-0435.2020.06.006

Acta Agrestia Sinica ›› 2020, Vol. 28 ›› Issue (6): 1535-1543.DOI: 10.11733/j.issn.1007-0435.2020.06.006

Incidence of Oat Powdery Mildew and Pathogen Identification in the Main Oat Production Areas of Gansu Province

SUN Hao-yang¹, ZHAO Gui-qin¹, ZHANG Jian-gui¹, LI Xin², YANG Yan-shan¹, CHAI Ji-kuan¹, ZENG Liang¹, JIAO Run-an¹, JIN Xiao-wen¹, LI Rong¹, GONG Wen-long¹

1. Key Laboratory of Grassland Ecology System, Ministry of Education, Sino-U. S. Centers for Grazing Land Ecosystem Sustainability, College of Pratacultural Science, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China;
2. Agronomy College, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China

Received:2020-05-23 Revised:2020-07-05 Online:2020-12-15 Published:2020-12-02

甘肃省燕麦主产区白粉病调查及病原鉴定

孙浩洋¹, 赵桂琴¹, 张建贵¹, 李鑫², 杨琰珊¹, 柴继宽¹, 曾亮¹, 焦润安¹, 金小雯¹, 黎蓉¹, 宫文龙¹

1. 甘肃农业大学草业学院, 草业生态系统教育部重点实验室, 甘肃省草业工程实验室, 中-美草地畜牧业可持续发展研究中心, 甘肃兰州 730070;
2. 甘肃农业大学农学院, 甘肃兰州 730070

通讯作者: 赵桂琴
作者简介:孙浩洋(1993-),男,汉族,陕西富平人,硕士,主要从事牧草种质资源与育种研究,E-mail:sun951hy@foxmail.com
基金资助:
国家燕麦荞麦产业技术体系（CARS-8-C）；盛彤笙创新基金（GSAU-STS-2018-21）共同资助

Abstract

Abstract: Oat powdery mildew is a popular wider,onset heavier oat diseases all over the world. To pinpoint the occurrence situation and damage in the main production area of oat (Avena sativa) in Gansu province of northern China. The occurrence of oat powdery mildew in each leaf was investigated,and leaves were collected from the field for pathogen identification. The investigation was located in Tongwei County of Dingxi City,Shandan and Minle Counties of Zhangye City,Tianzhu County of Wuwei City,and Gannan County of Hezuo City. The results showed that the powdery mildew occurred in most survey counties except for Hezuo and Luqu. ‘Sweet oat’ had the highest disease index (50.10) in Tianzhu County and the disease index of ‘Mulesi’ and ‘Jiayan No.2’ in Shandan County was lower (0.28 and 0.34,respectively). The incidence of the same oat varieties was significantly different in different survey areas. The disease index of ‘Sweet oat’ in Tianzhu County is 3.40~50.10;that of ‘Baiyan 7’ in Yongdeng County was 43.29,and only 1.22 in Tongwei County. The differences of disease severity among different oat varieties in the same area were observed. Combined with morphological and molecular biological methods,the partial sequence of oat powdery mildew pathogens collected during the investigation was analyzed and two different powdery mildew specific types were identified,the one was Blumeria. graminis (DC.) f.sp. secalis,the other was B. graminis (DC.) f.sp. avenae.

Key words: Oat, Main production area, Powdery mildew, Disease investigation, Pathogenic identification

摘要： 为了解甘肃省燕麦（Avena sativa）产区白粉病的发生及其危害情况，本研究对张掖、甘南、武威和定西等市（州）主要燕麦产区进行了白粉病调查，同时采集病样对白粉病病原进行鉴定。结果表明，除甘南州的合作市和碌曲县未发现白粉病外，其余调查县（市）均有发生，其中天祝县种植的‘甜燕麦’病情指数最高可达50.10，山丹县种植的‘牧乐思’和‘加燕2号’病情指数较低，分别为0.28和0.34；同一燕麦品种在不同调查区发病程度差异较大，其中‘甜燕麦’病情指数变化最大（3.40~50.10），‘白燕7号’在永登县病情指数高达43.29，在通渭县仅为1.22；不同品种在同一种植区的发病程度也有差异。结合形态学和分子生物学方法对调查过程中采集的白粉病病原菌进行鉴定，发现甘肃省燕麦白粉病可能存在禾本科布氏白粉菌黑麦专化型Blumeria.graminis（DC.）f.sp.secalis和禾本科布氏白粉菌燕麦专化型B.graminis（DC.）f.sp.avenae共2种。

关键词: 燕麦, 主产区, 白粉病, 病情调查, 病原菌鉴定

CLC Number:

S152.6

SUN Hao-yang, ZHAO Gui-qin, ZHANG Jian-gui, LI Xin, YANG Yan-shan, CHAI Ji-kuan, ZENG Liang, JIAO Run-an, JIN Xiao-wen, LI Rong, GONG Wen-long. Incidence of Oat Powdery Mildew and Pathogen Identification in the Main Oat Production Areas of Gansu Province[J]. Acta Agrestia Sinica, 2020, 28(6): 1535-1543.

孙浩洋, 赵桂琴, 张建贵, 李鑫, 杨琰珊, 柴继宽, 曾亮, 焦润安, 金小雯, 黎蓉, 宫文龙. 甘肃省燕麦主产区白粉病调查及病原鉴定[J]. 草地学报, 2020, 28(6): 1535-1543.

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References

[1] 何家泌,宋玉立,张忠山,等. 小麦白粉病及其防治I.小麦白粉病的分布、症状和危害[J]. 河南农业科学,1998(01):18-19
[2] 李帅,朱路路,李景富,等. 黑龙江省番茄白粉病病原鉴定[J]. 植物保护,2014,40(04):112-114
[3] 张怡,马晓萌,张佩佩,等. 周口地区油菜白粉病病原的鉴定[J]. 植物保护,2012,38(03):32-35
[4] 袁玉涛,史娟,马新,等. 紫花苜蓿白粉病病原菌鉴定及其生物学特性[J]. 微生物学通报,2020:1-12
[5] Xue L H,Li C J,Zhao G Q. First Report of Powdery Mildew Caused by Blumeria Graminis on Avena Sativa in China[J]. Plant Disease,2017,101(11):1954
[6] 孙道旺,尹桂芳,卢文洁,等. 云南省燕麦白粉病病原鉴定及致病力测定[J]. 植物保护学报,2017,44(04):617-622
[7] 周启龙,多吉顿珠,益西央宗. 拉萨地区16个燕麦引进品种的灰色关联度评价[J]. 草地学报,2020,28(02):389-396
[8] 汪焱,张英,苏贝贝,等. 高寒区不同地域燕麦根际土壤微生物多样性研究[J]. 草地学报,2020,28(02):358-366
[9] 南铭,赵桂琴,李晶,等. 西北半干旱区引种燕麦品种产量与品质的关联分析及评价[J]. 草地学报,2018,26(01):125-133
[10] Chowdhury J,Henderson M,Schweizer P,et al. Differential Accumulation of Callose,Arabinoxylan and Cellulose in Nonpenetrated Versus Penetrated Papillae on Leaves of Barley Infected with Blumeria Graminis f. sp. Hordei[J]. New Phytologist,2014,204(3):650-660
[11] 任长忠,胡跃高. 中国燕麦学[M]. 北京:中国农业出版社,2013:5
[12] 王丽,霍治国,张蕾,等. 气候变化对中国农作物病害发生的影响[J]. 生态学杂志,2012,31(07):1673-1684
[13] 孙浩洋,赵桂琴,柴继宽,等. 种植区环境对燕麦种质成株期白粉病抗性的影响[J]. 草原与草坪,2019,39(04):31-38
[14] 赵峰,郭满库,郭成,等. 213份燕麦种质的白粉病抗性评价[J]. 草业科学,2017,11(2):331-338
[15] 郭斌,郭满库,郭成,等. 燕麦种质资源抗白粉病鉴定及利用评价[J]. 植物保护,2012,38(4):144-146
[16] 孙道旺,李春花,尹桂芳,等. 播期和密度对云南冬播燕麦主要经济性状及产量的影响[J]. 中国农学通报,2019,35(36):21-25
[17] 霍治国,陈林,刘万才,等. 中国小麦白粉病发生地域分布的气候分区[J]. 生态学报,2002,22(11):1873-1881
[18] Gu Y,Li Y,Wang C,et al. Inter-Seasonal and Altitudinal Inoculum Dynamics for Wheat Stripe Rust and Powdery Mildew Epidemics in Gangu,Northwestern China[J]. Crop Protection,2018,110:65-72
[19] 农业部农药检定所. GB/T 17980.22-2000农药田间药效试验准则(一)杀菌剂防治禾谷类白粉病[S]. 北京:中国标准出版社,2000:433-436
[20] 沈平,陈向东. 微生物学实验[M]. 第四版. 北京:高等教育出版社,2007:73-74
[21] White T J,Bruns T,Lee S,et al. 38-Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics[M]. San Diego:Academic Press,1990:315-322
[22] Takamatsu S,Kano Y. PCR Primers Useful for Nucleotide Sequencing of rDNA of the Powdery Mildew Fungi[J]. Mycoscience,2001,42(1):135-139
[23] Mori Y,Sato Y,Takamatsu S. Evolutionary Analysis of the Powdery Mildew Fungi Using Nucleotide Sequences of the Nuclear Ribosomal DNA[J]. Mycologia,2000,92(1):74-93
[24] 曹世勤,何理,陈杰新,等. 2015-2017年平凉市崆峒区小麦白粉病越冬调查[J].甘肃农业科技,2018(01):5-8
[25] 金柳艳,孙华,石洁,等. 2016年黄淮海地区主栽品种抗病监测与现状分析[J]. 玉米科学,2018,26(04):150-155
[26] 黄凌洪,张天才,汪锐辉,等. 不同生态环境下2009年江西省主栽早稻品种稻瘟病的发生[J]. 江西农业大学学报,2010,32(03):485-488
[27] 张蕾,郭安红,李森. 小麦白粉病气候变化响应及动态预警[J]. 自然灾害学报,2017,26(01):141-148
[28] 刘伟,姚冬明,范洁茹,等. 田间空气中小麦白粉菌分生孢子的动态监测研究[J]. 植物病理学报,2016,46(01):112-118
[29] Uloth M B,You M P,Barbetti M J. Plant Age and Ambient Temperature:Significant Drivers for Powdery Mildew (Erysiphe Cruciferarum) Epidemics On Oilseed Rape (Brassica Napus)[J]. Plant Pathology,2018,67(2):445-456
[30] 黄苗苗. 甘肃省小麦白粉病菌的温度敏感性及其抗病性遗传分析[D]. 兰州:甘肃农业大学,2015:18-19
[31] Okoń S,Paczos-Grzeda E,Ociepa T,et al. Avena sterilis L. Genotypes as a Potential Source of Resistance to Oat Powdery Mildew[J]. Plant Disease,2016,100(10):2145-2151
[32] Herrmann M H,Mohler V. Locating Two Novel Genes for Resistance to Powdery Mildew from Avena byzantina in the Oat Genome[J]. Plant Breeding,2018,137(6):832-838
[33] Okoń S M. Effectiveness of Resistance Genes to Powdery Mildew in Oat[J]. Crop Protection,2015,74:48-50
[34] Siahaan S A S,Sakamoto H,Shinoda T,et al. Geographic and Temporal Distributions of Four Genotypes Found in Erysiphe Gracilis Var. Gracilis,a Powdery Mildew of Evergreen Oaks (Erysiphales)[J]. Mycoscience,2018,59(2):110-118
[35] Wu H,Pan Y,Di R,et al. Molecular Identification of the Powdery Mildew Fungus Infecting Rubber Trees in China[J]. Forest Pathology,2019,49(5):e12519
[36] 李敏权,张自和,柴兆祥,等. 紫花苜蓿白粉病病原鉴定[J]. 甘肃农业大学学报,2002,37(3):303-306
[37] 张蓉,马建华,杨芳先,等. 宁夏苜蓿主要病害田间发生规律的研究[J]. 石河子大学学报(自然科学版),2004(S1):168-171
[38] 丁锦平,张庆琛,裴冬丽,等. 枸杞白粉病的病原菌鉴定[J]. 江苏农业科学,2015,43(06):137-139
[39] 干射香,章松柏,彭小琴,等. 北京地区枸杞白粉病病原菌的鉴定[J]. 长江大学学报(自然科学版),2019,16(09):67-70
[40] 董毓平,姜文涛,柳建,等. 吉林省枸杞白粉病病原菌鉴定[J]. 菌物研究,2016(03):149-152
[41] Inuma T,Khodaparast S A,Takamatsu S. Multilocus Phylogenetic Analyses within Blumeria Graminis,a Powdery Mildew Fungus of Cereals[J]. Molecular Phylogenetics and Evolution,2007,44(2):741-751
[42] 刘建利. 利用28S rDNA D1/D2区和ITS rDNA序列鉴定甜瓜白粉病病原菌[J]. 植物保护学报,2011,38(01):47-51
[43] 韩宁宁,黄元腾吉,李峰,等. 南宁市桑白粉病病原菌种类鉴定[J]. 植物保护,2019,45(03):67-70
[44] 陶家凤,文成敬,张敏,等. 小麦白粉菌对几种禾本科植物侵染的观察[J]. 四川农业大学学报,1985(02):1-8
[45] Walker A S,Bouguennec A,Confais J,et al. Evidence of Host-Range Expansion from New Powdery Mildew (Blumeria Graminis) Infections of Triticale (×Triticosecale) in France[J]. Plant Pathology,2011,60(2):207-220
[46] Menardo F,Praz C R,Wyder S,et al. Hybridization of Powdery Mildew Strains Gives Rise to Pathogens on Novel Agricultural Crop Species[J]. Nature Genetics,2016,48:201
[47] 曹学仁,车海彦,罗大全. 海南橡胶树白粉菌的群体遗传结构研究[J]. 植物保护,2019,45(02):121-124
[48] Müller K,Mcdermott J M,Wolfe M S,et al. Analysis of Diversity in Populations of Plant Pathogens:The Barley Powdery Mildew Pathogen Across Europe[J]. European Journal of Plant Pathology,1996,102(4):385-395
[49] 朱靖环,杨建明,汪军妹,等. 大麦白粉菌致病型鉴定初报[J]. 浙江农业学报,2007,19(1):29-33

Incidence of Oat Powdery Mildew and Pathogen Identification in the Main Oat Production Areas of Gansu Province

甘肃省燕麦主产区白粉病调查及病原鉴定

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