Study on the Cold Resistance of New Strains of Timothy Grass from the Central Part of Gansu Province

doi:10.11733/j.issn.1007-0435.2021.03.025

Acta Agrestia Sinica ›› 2021, Vol. 29 ›› Issue (3): 625-630.DOI: 10.11733/j.issn.1007-0435.2021.03.025

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Study on the Cold Resistance of New Strains of Timothy Grass from the Central Part of Gansu Province

YIN Yu-lin, MA Wen-xin, LI Ya-juan, TIAN Xin-hui, DU Wen-hua

Pratacultural Science College of Gansu Agricultural University, Grassland Ecosystem Key Laboratory of Ministry of Education, Si-no-U. S. Research Centers for Sustainable Grassland and Livestock Management, Lanzhou, Gansu Province 730070, China

Received:2020-08-31 Revised:2020-10-24 Online:2021-03-15 Published:2021-04-02

甘肃省中部地区猫尾草新品系的抗寒性研究

尹玉林, 马文馨, 李亚娟, 田新会, 杜文华

甘肃农业大学草业学院/草业生态系统教育部重点实验室/中-美草地畜牧业可持续发展研究中心, 甘肃兰州 730070

通讯作者: 杜文华
作者简介:尹玉林(1996-),男,云南省腾冲人,硕士研究生,主要从事农艺与种业研究,E-mail:572071338@qq.com
基金资助:
国家重点研发计划（2018YFD0502402-3）；西藏饲草产业专项（XZ201801 NA02，XZ201901 NA03）；甘肃省草地畜牧业可持续发展创新团队项目（2017C-11）；现代农业产业技术体系建设专项资金（CARS-40-09B）共同资助

Abstract

Abstract: In this experiment,a new line of Timothy (Phleum pratense) (T) cultivated by Gansu Agricultural University was used as the test material,and the variety of Timothy ‘Sichuan’(CK1) and Timothy ‘Minshan’(CK2) were used as controls. The changes of physiological and biochemical indexes of the three materials under low temperature stress (0℃,-10℃,-20℃,-30℃),their cold resistance strength were studied in order to cultivate high-yield,high-quality,and stress-resistant timothy breeds. The results showed that low-temperature treatments had significant effects on the soluble sugar (SS),peroxidase (POD) and catalase (CAT) contents of the tested timothy (P<0.05);but had no significant effect on malondialdehyde (MDA),superoxide dismutase (SOD),or chlorophyll (CHL) content. With the intensification of the stress,the SS content of the test materials showed a trend of first decreasing and then increasing,the content was the lowest at -20℃ (8.6 mg·g^-1),and the POD activity showed a trend of first increasing and then decreasing,at -20℃ the activity was the highest (606.23 U·g^-1·min^-1),and the CAT activity increased under cold exposure. The SS content,SOD and POD of T was significantly higher than that of CK1 and CK2 (P<0.05) under the treatments of -10℃ to -30℃. Overall,the new timothy strain (T) has strong cold resistance and is suitable for further regional and production trials.

Key words: Phleun pratense, New strain, Breeding of variety, Physiological index, Evaluation of cold resistance

摘要： 本试验以甘肃农业大学培育的猫尾草(Phleum pratense)新品系(T)为试验材料，‘川西’猫尾草品种(CK1)和‘岷山’猫尾草品种(CK2)为对照，研究3个材料在低温胁迫(0℃，—10℃，—20℃，—30℃)下生理生化指标的变化，明晰其抗寒性强弱，为培育高产优质、抗性强的猫尾草新品系打下基础。结果表明：不同低温处理对供试猫尾草的可溶性糖(Soluble sugar,SS)含量、过氧化物酶(Peroxidase,POD)和过氧化氢酶(Catalase,CAT)活性均有显著影响(P<0.05)；对超氧化物歧化酶(Superoxide dismutase,SOD)活性、丙二醛(Malondialdehyde,MDA)和叶绿素(Chlorophyll,CHL)含量无显著影响。随着胁迫的加剧，供试材料的SS含量呈先降后升的趋势，在—20℃时含量最低(8.6 mg·g^-1)，POD活性呈先升后降趋势，在—20℃时活性最高(606.23 U·g^-1·min^-1)，CAT活性呈上升趋势。在—10℃至—30℃处理下，T的SS含量均显著高于CK1和CK2(P<0.05)，且不同低温胁迫处理下T的SOD，POD活性均显著高于CK1和CK2(P<0.05)。综合表明，猫尾草新品系(T)的抗寒性较强，适宜开展进一步的区域试验和生产试验。

关键词: 猫尾草, 新品系, 品种选育, 生理指标, 抗寒性评价

CLC Number:

S543+.1

YIN Yu-lin, MA Wen-xin, LI Ya-juan, TIAN Xin-hui, DU Wen-hua. Study on the Cold Resistance of New Strains of Timothy Grass from the Central Part of Gansu Province[J]. Acta Agrestia Sinica, 2021, 29(3): 625-630.

尹玉林, 马文馨, 李亚娟, 田新会, 杜文华. 甘肃省中部地区猫尾草新品系的抗寒性研究[J]. 草地学报, 2021, 29(3): 625-630.

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Study on the Cold Resistance of New Strains of Timothy Grass from the Central Part of Gansu Province

甘肃省中部地区猫尾草新品系的抗寒性研究

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