Acta Agrestia Sinica ›› 2021, Vol. 29 ›› Issue (10): 2233-2239.DOI: 10.11733/j.issn.1007-0435.2021.10.014

Previous Articles     Next Articles

Effects of H2S on Physiological Characteristics and Endogenous Hormones in Tall Fescue Seedlings under Low-light Stress

GAO Shuang-hong, LI Yuan-ying, LIU Bo-wen, LONG Si, LIU Tie-yuan, XU Yue-fei   

  1. College of Grassland Agriculture, Northwest A&F University, Yangling, Shaanxi Province 712100, China
  • Received:2021-05-31 Revised:2021-06-30 Online:2021-10-15 Published:2021-11-05

H2S对弱光胁迫下高羊茅幼苗生理特性和内源激素的影响

高双红, 李媛英, 刘博文, 龙思, 刘铁芫, 许岳飞   

  1. 西北农林科技大学草业与草原学院, 陕西 杨凌 712100
  • 通讯作者: 许岳飞,E-mail:xuyuefei@nwsuaf.edu.cn
  • 作者简介:高双红(1994-),女,汉族,甘肃靖远人,硕士研究生,主要从事牧草、草坪草逆境生物学研究,E-mail:2965722717@qq.com
  • 基金资助:
    自然科学基金项目(32071888)资助;中央高校基本科研业务费专项资金(2452019080)资助;陕西省重点研发计划-一般项目(2021 NY-003)资助;陕西省重点研发计划-重点产业链(2021ZDLNY02-04;2020ZDLNY02-05)资助

Abstract: This study evaluated the effects of exogenous hydrogen sulfide (H2S) on the physiological characteristics and endogenous hormones in tall fescue (Festuca arundinacea schyeb. cv. ‘Arid 3’) seedlings under low-light stress. The seedlings of Arid 3 under low-light stress were sprayed with sodium hydrosulfide (NaHS, H2S donor), hypotaurine (HT, H2S scavenger), aminooxy acetic acid (AOA, H2S biosynthesis inhibitor), and distilled water respectively;and the indicators of germination characteristics, membrane stability, root vitality and endogenous hormones were determined. The results showed that exogenous H2S significantly increased the germination rate, germination potential, vitality index, endogenous H2S and abscisic acid (ABA) contents under low-light stress. Meanwhile, malondialdehyde (MDA) and superoxide anion (O2·-), relative electrolyte leakage, and gibberellin (GA) levels were decreased by 17.5%, 11.0%, and 23.0%, respectively. However, the HT or AOA treatment inhibited the effects of exogenous H2S. It indicates that exogenous H2S can increase ABA level, improve germination characteristics, maintain the membrane stability and integrity under low-light stress, thereby alleviate oxidative damage and enhance the adaption to low-light stress in tall fescue seedlings.

Key words: Tall fescue, Low light, Hydrogen sulfide, Physiological characteristics, Endogenous hormones

摘要: 为了探讨外源硫化氢(H2S)在弱光胁迫下对高羊茅(Festuca arundinacea)生理特性和内源激素的调节作用,本文以高羊茅品种Arid 3号为试验材料,在弱光胁迫下分别喷施外源H2S供体(NaHS)、H2S清除剂次牛磺酸(HT)、H2S合成酶抑制剂羧甲基胺半盐酸盐(AOA)和蒸馏水,测定其对高羊茅萌发特性、细胞膜稳定性、根活力和内源激素的影响。结果表明:弱光下施加H2S显著提高了发芽率、发芽势、活力指数、内源H2S和脱落酸(ABA)含量,同时降低了丙二醛(MDA)、细胞膜透性和超氧阴离子(O2·-),且较单独遮荫处理分别降低了17.5%,23.0%和11.0%;而施加HT和AOA会抑制内源H2S的效果。综上,H2S能够提高弱光胁迫下高羊茅的ABA水平,增强萌发特性,维持细胞膜的稳定性和完整性,从而减轻弱光胁迫引起的细胞膜氧化损伤,提高高羊茅对弱光胁迫的适应能力。

关键词: 高羊茅, 弱光胁迫, 硫化氢, 生理特性, 内源激素

CLC Number: