Identification and Characteristics of Phosphate-solubilizing Endophytic Bacteria from Root Nodules of Lespedeza daurica ‘Jinnong No.1’

doi:10.11733/j.issn.1007-0435.2022.12.009

Acta Agrestia Sinica ›› 2022, Vol. 30 ›› Issue (12): 3241-3252.DOI: 10.11733/j.issn.1007-0435.2022.12.009

Identification and Characteristics of Phosphate-solubilizing Endophytic Bacteria from Root Nodules of Lespedeza daurica ‘Jinnong No.1’

YANG Kai-yuan, HUANG Chen, GAO Peng, LIANG Yin-ping, HAN Ling-juan, ZHAO Xiang

College of grassland science, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China

Received:2022-07-12 Revised:2022-10-26 Published:2023-01-04

‘晋农1号’达乌里胡枝子根瘤内生解磷菌的鉴定及特性研究

杨凯元, 黄臣, 高鹏, 梁银萍, 韩玲娟, 赵祥

山西农业大学草业学院, 山西太谷 030801

通讯作者: 赵祥,Email:sxndzhaox@126.com
作者简介:杨凯元(1998-),男,汉族,河北容城人,硕士研究生,主要从事草地微生物研究,E-mail:1632021098@qq.com
基金资助:
国家自然科学基金(31870438);山西农业大学科技创新基金项目(2018YJ37);山西省2021年度研究生教育创新项目(2021Y344)资助

Abstract

Abstract: Endophytic phosphate-solubilizing bacteria isolated from root nodules could promote stress resistance,nodulation,and nitrogen fixation in plants. In this study,Lespedeza daurica ‘Jinnong No.1’ was used as the experimental material. Nodule endophytic bacteria were isolated by plate streaking,the bacteria with phosphate-solubilizing abilities were selected and the phosphate-solubilizing abilities of initially selected bacteria were determined. Then the stress resistance and plant growth-promoting characteristics were determined. The results showed that:eighty-eight endophytic bacterial strains were isolated from root nodules of Lespedeza daurica,among which eleven strains possessed phosphate-solubilizing abilities. The organic phosphorus solubility of TG41,TG43,TG47,and TG68 were 0.73~0.86 μg·mL^-1;the inorganic phosphorus solubility of TG47 and TG68 were 72.97 and 1.29 μg·mL^-1,respectively,the four strains were identified as effective phosphate-solubilizing bacteria. TG41 and TG43 were determined as Bacillus zanthoxyli,TG47 as Phyllobacterium zundukense and TG68 as Pantoea agglomerans. TG41,TG43,and TG68 could grow with NaCl content of 0.01%~9.00% and pH of 4~12. All 4 strains were not resistant to high temperature,but TG47 and TG68 could grow at 4℃. TG41,TG43,and TG68 could secrete siderophore and organic acids. In summary,there were abundant endophytic bacteria in root nodules of Lespedeza daurica and some of them possessed phosphate-solubilizing abilities,stress resistance,and growth-promoting potential,which could be used to research and develop composite microbial inoculants for rhizobia suitable for planting Lespedeza daurica in the Loess Plateau.

Key words: Lespedeza daurica, Nodule endophytic bacteria, Phosphate-solubilizing bacteria, Stress resistance, Plant growth promoting

摘要： 根瘤内生解磷菌具有促进豆科植物结瘤固氮，提高植物抗逆性等作用。本研究以‘晋农1号’达乌里胡枝子(Lespedeza daurica ‘Jinnong NO.1’)为试验材料，采用“划线法”分离其根瘤内生菌，筛选具有解磷能力的菌株，测定其解磷能力，并对其抗逆和促生特性进行研究。结果表明：达乌里胡枝子根瘤中共分离出88株内生菌，其中11株具有解磷能力；TG41，TG43，TG47和TG68解有机磷量为0.73~1.29μg·mL^-1，TG47和TG68解无机磷量为72.97和1.29μg·mL^-1，为有效解磷菌；经鉴定，TG41和TG43为Bacillus zanthoxyli，TG47为Phyllobacterium zundukense，TG68为Pantoea agglomerans；TG41，TG43和TG68在盐浓度0.01%~9.00%和pH4~pH12条件下均可生长；4株菌不耐高温，TG47和TG68能在4℃生长；TG41，TG43和TG68能分泌铁载体和有机酸。综上，达乌里胡枝子根瘤中存在丰富的内生菌，部分具有解磷功能、抗逆和促生潜力，可用于研发适合黄土高原地区胡枝子建植的根瘤微生物复合菌剂。

关键词: 达乌里胡枝子, 根瘤内生菌, 解磷菌, 抗逆, 促生效应

CLC Number:

S541+.5

YANG Kai-yuan, HUANG Chen, GAO Peng, LIANG Yin-ping, HAN Ling-juan, ZHAO Xiang. Identification and Characteristics of Phosphate-solubilizing Endophytic Bacteria from Root Nodules of Lespedeza daurica ‘Jinnong No.1’[J]. Acta Agrestia Sinica, 2022, 30(12): 3241-3252.

杨凯元, 黄臣, 高鹏, 梁银萍, 韩玲娟, 赵祥. ‘晋农1号’达乌里胡枝子根瘤内生解磷菌的鉴定及特性研究[J]. 草地学报, 2022, 30(12): 3241-3252.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2022.12.009

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2022/V30/I12/3241

References

[1] LI J H,WANG E T,CHEN W F,et al. Genetic Diversity and Potential for Promotion of Plant Growth Detected in Nodule Endophytic Bacteria of Soybean Grown in Heilongjiang Province of China[J]. Soil Biology & Biochemistry,2008,40(1):238-246
[2] HERRIDGE D F,PEOPLES M B,BODDEY R M. Global Inputs of Biological Nitrogen Fixation in Agricultural Systems[J]. Plant & Soil,2008,311(1-2):1-18
[3] 杨庆,王义祥,李欣欣,等. 酸性土壤中决明属牧草高效根瘤菌的分离及筛选[J]. 草地学报,2020,28(6):1519-1526
[4] SINGH Z,SINGH G,AGGARWAL N,et al. Symbiotic Efficiency vis-à-vis Chickpea Performance as Affected by Seed Inoculation with Mesorhizobium,Phosphorus-solubilizing Bacteria and Phosphorus Application[J]. Journal of Plant Nutrition,2021,45(2):246-258
[5] HERNANDEZ G,VALDES-LOPEZ O,RAMIREZ M,et al. Global Changes in the Transcript and Metabolic Profiles During Symbiotic Nitrogen Fixation in Phosphorus-stressed Common Bean Plants[J]. Plant Physiology,2009,151(3):1221-1238
[6] NEILA A,ADNANE B,BOULBABA L,et al. Rhizobial Symbiosis Efficiency Increases in Common Bean under Sparingly Soluble Calcium-phosphorus[J]. Journal of Plant Nutrition,2017,40(13):1829-1841
[7] THAMIR S A N,MICHAEL L K,TIMOTHY R M. Phosphorus Uptake by Bean Nodules[J]. Plant & Soil,1998,198(1):71-78
[8] BHATTACHARYYA P,JHA D. Plant Growth-promoting Rhizobacteria (PGPR):Emergence in Agriculture[J]. World Journal of Microbiology & Biotechnology,2012,28(4):1327-1350
[9] ALMAS Z,MOHAMMAD S K,ASFA R,et al. Role of Phosphate-solubilizing Bacteria in Legume Improvement[M]. Microbes for Legume Improvement. Cham:Springer,2017:175-197
[10] ELIZABETH P,MIGUEL S,MARIA M B,et al. Isolation and Characterization of Mineral Phosphate-solubilizing Bacteria Naturally Colonizing a Limonitic Crust in the South-eastern Venezuelan Region[J]. Soil Biology & Biochemistry,2007,39(11):2905-2914
[11] MEYER S D,BEUF K D,VEKEMAN B,et al. A Large Diversity of Non-rhizobial Endophytes Found in Legume Root Nodules in Flanders (Belgium)[J]. Soil Biology & Biochemistry,2015,83:1-11
[12] MARTINEZ-HIDALGO P,HIRSCH A. The Nodule Microbiome:N₂-fixing Rhizobia Do Not Live Alone[J]. Phytobiomes Journal,2017,1(2):70-82
[13] 邱并生. 根瘤内生细菌[J]. 微生物学通报,2013,40(3):544
[14] 赵龙飞,徐亚军,曹冬建,等. 溶磷性大豆根瘤内生菌的筛选、抗性及系统发育和促生[J]. 生态学报,2015,35(13):4425-4435
[15] JULIA K S,WELINGTON L A,Rodrigo M,et al. Isolation and Characterization of Soybean-associated Bacteria and Their Potential for Plant Growth Promotion[J]. Environmental Microbiology,2010,6(12):1244-1251
[16] YASMEEN S,BANO A. Combined Effect of Phosphate-solubilizing Microorganisms,and on Root Nodulation and Physiology of Soybean (Glycine max L.)[J]. Communications in Soil Science & Plant Analysis,2014,45(18):2373-2384
[17] ELKOCA E,KANTAR F,SAHIN F. Influence of Nitrogen Fixing and Phosphorus Solubilizing Bacteria on the Nodulation,Plant Growth,and Yield of Chickpea[J]. Journal of Plant Nutrition,2007,31(1):157-171
[18] RAJPUT M,PANDYA M,RAJKUMAR S. Exploring Plant Growth Promoting Potential of Non Rhizobial Root Nodules Endophytes of Vigna radiata¹[J]. Microbiology,2015,84(1):80-89
[19] 黄臣,杨凯元,高鹏,等. 达乌里胡枝子根际解磷细菌的筛选、鉴定及特性研究[J]. 草地学报,2022,30(9):2345-2355
[20] PALANIAPPAN P,CHAUHAN P S,SARAVANAN V S,et al. Isolation and Characterization of Plant Growth Promoting Endophytic Bacterial Isolates from Root Nodule of Lespedeza sp.[J]. Biology & Fertility of Soils,2010,46(8):807-816
[21] SUBRAMANIAN P,KIN K,KRISHNAMOORTHY R,et al. Endophytic Bacteria Improve Nodule Function and Plant Nitrogen in Soybean on Co-inoculation with Bradyrhizobium japonicum MN110[J]. Plant Growth Regulation,2015,76(3):327-332
[22] BUSBY R R,RODRIGUEZ G,GEBHART D L,et al. Native Lespedeza Species Harbor Greater Non-rhizobial Bacterial Diversity in Root Nodules Compared to the Coexisting Invader,L. cuneata[J]. Plant & Soil,2016,401(1-2):427-436
[23] 闫剑利. 山西为国家级草育成新品种[N]. 科学导报,2014-06-24(A01)
[24] 冀玉良,韦革宏. 陕西商州铅锌尾矿区豆科植物根瘤菌耐铅锌胁迫能力及其生理生化特征[J]. 干旱地区农业研究,2014,32(4):9-13
[25] 苗阳阳,周彤,师尚礼,等. 硼对根瘤菌在紫花苜蓿体内运移和定殖及对幼苗生长的影响[J]. 草业学报,2017,26(4):120-133
[26] 东秀珠,蔡妙英. 常见细菌系统和鉴定手册[M]. 北京:科学出版社,2001:353-384
[27] 王平,董飚,李阜棣,等. 小麦根圈细菌铁载体的检测[J]. 微生物学通报,1994(6):323-326
[28] 康宏,郭艾琳,马梦彪,等. 产有机酸采油菌的筛选及产酸情况的分析[J]. 生物技术,2011,21(4):89-93
[29] 张晓波. 草地早熟禾根际促生菌(PGPR)特性及根际微生物区系研究[D]. 北京:北京林业大学,2008:58-60
[30] 沈萍,陈向东. 微生物学(第2版)[M]. 北京:高等教育出版社,2006:85-92
[31] BAKHTIYARIFAR M,ENAYATIZAMIR N,KHANLOU K M. Biochemical and Molecular Investigation of Non-rhizobial Endophytic Bacteria as Potential Biofertilisers[J]. Archives of Microbiology,2021,203(1):513-521
[32] 冀玉良,韦革宏. 商洛多花胡枝子根瘤菌16S rDNA-RFLP分析及系统发育研究[J]. 西北植物学报,2010,30(5):925-932
[33] 陈文峰. 根瘤菌系统学研究进展与展望[J]. 微生物学通报,2016,43(5):1095-1100
[34] ANTHONY C Y,ERIC W T. Geographic and Environmental Sources of Variation in Lake Bacterial Community Composition[J]. Applied & Environmental Microbiology,2005,71(1):227-239
[35] DAVE S,LIEVEN W,EVA M T,et al. Impact of Agricultural Practices on the Zea mays L. Endophytic Community[J]. Applied & Environmental Microbiology,2004,70(3):1475-1482
[36] HANI A,CHANTAL J B,NADIA G,et al. Potential of Rhizobium and Bradyrhizobium Species as Plant Growth Promoting Rhizobacteria on Non-legumes:Effect on Radishes (Raphanus sativus L.)[M]. Molecular Microbial Ecology of The Soil. Dordrecht:Springer,1998:57-67
[37] 张希涛,康丽华,马海宾,等. 具有解磷能力的相思根瘤菌的筛选[J]. 林业科学研究,2008(5):619-624
[38] 陈晓琳,刘洁莹,宋艳琦,等. 高效解磷根瘤菌菌株的分离筛选及对花榈木苗木生长的影响[J]. 中南林业科技大学学报,2022,42(4):76-82+92
[39] 韩梅,于芳,肖亦农,等. 1株具解磷特性大豆根瘤菌的分离筛选与回接鉴定[J]. 微生物学杂志,2010,30(2):51-56
[40] AREGU A A,LENNA A R,FASSIL A,et al. Diversity of Sporadic Symbionts and Nonsymbiotic Endophytic Bacteria Isolated from Nodules of Woody,Shrub,and Food Legumes in Ethiopia[J]. Applied Microbiology & Biotechnology,2013,97(23):10117-10134
[41] 蒲强,谭志远,彭桂香,等. 根瘤菌分类的新进展[J]. 微生物学通报,2016,43(3):619-633
[42] MOHSIN,T,SOHAIL H,TAHIRA Y,et al. Molecular Characterization and Identification of Plant Growth Promoting Endophytic Bacteria Isolated from the Root Nodules of Pea (Pisum sativum L.)[J]. World Journal of Microbiology & Biotechnology,2014,30(2):719-725
[43] 钟宇舟,余秀梅,陈强,等. 四川盆地大豆根瘤内生细菌的分离鉴定及促生效果[J]. 应用与环境生物学报,2017,23(1):46-53
[44] YANG L,LIU Y,CAO X,et al. Community Composition Specificity and Potential Role of Phosphorus Solubilizing Bacteria Attached on the Different Bloom-forming Cyanobacteria[J]. Microbiological Research,2017(205):59-65
[45] 池景良,郝敏,王志学,等. 解磷微生物研究及应用进展[J]. 微生物学杂志,2021,41(1):7
[46] MUNEES A,KIBRET M. Mechanisms and Applications of Plant Growth Promoting Rhizobacteria:Current Perspective[J]. Journal of King Saud University:Science,2014,26(1):1-20
[47] 陈倩,刘善江,白杨,等. 山西矿区复垦土壤中解磷细菌的筛选及鉴定[J]. 植物营养与肥料学报,2014,20(6):1505-1516
[48] 游银伟,王梅,江丽华,等. 高效解磷菌Bacillus subtilis P-1的N离子束诱变育种[J]. 西南农业学报,2009,22(4):1020-1022
[49] 梁振霆,唐婷. 内生菌对植物次生代谢产物的生物合成影响和抗逆功能研究[J]. 生物技术通报,2021,37(8):35-45
[50] GUILLERMO C C,MARIA E A C. Characterization of Siderophore-mediated Iron Transport from Rhizobium leguminosarum bv. phaseoli.[J]. Journal of Plant Nutrition,2000,23(11-12):1669-1683
[51] 穆文强,康慎敏,李平兰. 根际促生菌对植物的生长促进作用及机制研究进展[J]. 生命科学,2022,34(2):118-127
[52] ELLA M B,DAVID A D,PENELOPE M C S. Iron:An Essential Micronutrient for the Legume-rhizobium Symbiosis[J]. Frontiers in Plant Science,2013(4):359

Identification and Characteristics of Phosphate-solubilizing Endophytic Bacteria from Root Nodules of Lespedeza daurica ‘Jinnong No.1’

‘晋农1号’达乌里胡枝子根瘤内生解磷菌的鉴定及特性研究

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	MAO Yuan-yu, LI Li-jing, LI Jin-chao, ZHANG Jia-hang, DUAN Meng-qi, XU Li-xin. Gene Functional Analysis of ZjSGR Gene in Zoysia japonica Leaves in Response to Vitro Stress [J]. Acta Agrestia Sinica, 2022, 30(6): 1396-1402.
[2]	LI Hui-ping, GAN Ya-nan, HAN Qing-qing, YAO Dan, CHEN Jia, ZHANG Ming-xu, HE Ao-lei, ZHANG Jin-lin, ZHAO Qi. Isolation of Phosphate Solubilizing Bacteria from Soil of Spruce Forest in the Qilian Mountains and Their Growth Promotion Effects in White Clover [J]. Acta Agrestia Sinica, 2022, 30(4): 879-888.
[3]	HAN Fu-song, YU Cheng-qun, FU Gang, PENG Yi. Stress Resistance Mechanisms of Herbage under Low Temperature and Drought Stress [J]. Acta Agrestia Sinica, 2022, 30(11): 2856-2864.
[4]	FANG Ming-yue, WANG Yi-pan, ZHAO Yi, Reziya, Ai, SHEN Yu-hua, ZHANG Tie-jun. Morphological and Physiological Responses of Eight Alfalfa Cultivars to Combined Stress of Cold and Drought [J]. Acta Agrestia Sinica, 2022, 30(11): 2967-2974.
[5]	QU Song-lin, WU Yi-fan, LIU Zhong-kuan, WANG Guo-liang, CHEN Yan-jing, RONG Yu-ping. Research Progress for Effects of Arbuscular Mycorrhizal Fungi on Growth and Development of Alfalfa [J]. Acta Agrestia Sinica, 2022, 30(10): 2529-2534.
[6]	ZHAO Shu-dong, LI Jian-hong. The Study on Screening and Characteristics of Rhizosphere Growth-promoting Bacteria of Poa alpigena L. [J]. Acta Agrestia Sinica, 2021, 29(9): 1885-1891.
[7]	LI Qi, YAO Tuo, Abuman, YANG Xiao-mei, ZHANG Jian-gui, FENG Ying. Development of Plant Growth Promoting Rhizobacteria Microcapsules and Evaluation of Its Growth Promotion Effects on Alfalfa and Oats [J]. Acta Agrestia Sinica, 2019, 27(5): 1392-1399.
[8]	LI Fang, GAO Ping, DUAN Ting-yu. Response and Mechanism of Arbuscular Mycorrhizal Fungi to Abiotic Stress [J]. Acta Agrestia Sinica, 2016, 24(3): 491-500.
[9]	MA Wen-bin, YAO Tuo, RONG Liang-yan, WANG Guo-ji, SUN Guang-zheng, LIU Huan. Effects of PGPR Inoculum without Exogenous Nitrogen on the Growth and Root System Characteristics of Vicia sativa L. [J]. Acta Agrestia Sinica, 2015, 23(3): 496-501.
[10]	LI Yuan, YOU Yong-liang, ZHAO Hai-ming, XIE Nan, ZHAI Lan-ju, LIU Gui-bo. Analysis on the Yield， Quality and Stress Resistance of Brown Midrib Forage Sorghum Varieties [J]. Acta Agrestia Sinica, 2014, 22(4): 889-896.
[11]	ZHAO Mei-rong, SHEN Yu-hua, LI Yong-chun, DUAN Yong-ping. Research Progress in the Genetic Engineering of Alfalfa Stress Resistance [J]. , 2014, 22(2): 243-248.
[12]	ZHANG Yu-long, LU Xiao-liang, YANG Chen-de. A Preliminary Study on the Isolation and Characterization of Inorganic Phosphate-solubilizing Bacteria in Soil of Alpine Grasslands in Eastern Qilian Mountains [J]. , 2011, 19(4): 560-564.
[13]	LI Yu-e, YAO Tuo, RONG Liang-yan. Characteristics of IAA Secretion and Phosphate Dissolving of Phosphate-solubilizing Bacteria and Its Effect on Alfalfa Growth [J]. , 2010, 18(1): 84-88.
[14]	ZHAO Xiang, DONG Kuan-hu, ZHANG Yao, YANG Wu-de, LIANG Pi-fu. Study on Lamina Anatomical Structure of Lespedeza daurica(Laxm.) Schindl. from Different Populations [J]. , 2009, 17(4): 445-451.
[15]	ZHU Ying, YAO Tuo, LI Yu-e, SUN Hong-yang. Screening of Phosphate-solubilizing Bacteria and Their Acting Mechanisms in the Rhizosphere of Red Clover [J]. , 2009, 17(2): 259-263.