Response of Soil Bacterial Community Diversity and Co-occurrence Network to Foraging Intensity of Yaks in Alpine Meadow

doi:10.11733/j.issn.1007-0435.2023.01.002

Acta Agrestia Sinica ›› 2023, Vol. 31 ›› Issue (1): 9-18.DOI: 10.11733/j.issn.1007-0435.2023.01.002

Previous Articles Next Articles

Response of Soil Bacterial Community Diversity and Co-occurrence Network to Foraging Intensity of Yaks in Alpine Meadow

MENG Ling-xu, JIA Cai-ling, XU Chun-xue, ZHANG Xiao-wen, Huhe

College of Ecology and Environment, Inner Mongolia University, Hohhot, Inner Mongolia 010000, China

Received:2022-07-08 Revised:2022-08-26 Online:2023-01-15 Published:2023-02-07

高寒草甸土壤细菌群落多样性和共现性网络对牦牛觅食强度的响应

孟令旭, 贾彩玲, 许春雪, 张潇文, 呼和

内蒙古大学生态与环境学院, 内蒙古呼和浩特 010000

通讯作者: 呼和, E-mail:huhezhou@imu.edu.cn
作者简介:孟令旭(1998-)，男，汉族，山东临沂人，硕士研究生，主要从事微生物生态学和环境微生物学研究，E-mail：lxmeng0910@163.com
基金资助:
内蒙古自治区重点研发和成果转化计划(科技支撑东北振兴)课题(2022YFDZ)；内蒙古大学高层次人才启动基金(21800-5195116)；国家自然科学基金项目(31460029)资助

Abstract

Abstract: Yak foraging behavior is one of the most important factors affecting the Tibetan Plateau alpine meadow ecosystem,mainly through foraging,trampling,and dung deposition affecting the soil ecosystem. As an important component of soil ecosystems,soil bacterial communities play key roles in material cycling and energy flow. However,the mechanisms of bacterial community diversity and interactions in response to yak grazing are unclear. In this study,we analyzed the changes in bacterial community composition and its co-occurrence networks under different foraging intensities by long-term free grazing treatment of yaks in alpine meadows,and revealed the characteristics of soil bacterial community response to yak foraging behavior. The results showed that the dominant phyla of the bacterial community were Proteobacteria (30.66%~33.38%) and Actinobacteria (25.04%~28.99%),yak foraging significantly increased the relative abundance of Verrucomicrobia (2.46%~5.10%) and Gemmatimonadetes (1.91%~2.68%). In addition,yak foraging behavior significantly affected soil bacterial community beta diversity mainly by changing soil pH value,co-occurrence network complexity increased with foraging intensity,and an increase in the relative abundance of the Verrucomicrobia significantly reduced bacterial community beta diversity and network complexity. Yak foraging behavior can improve bacterial community stability,suggesting that appropriate foraging intensity can improve fragile alpine meadow ecosystems.

Key words: Yak foraging, Alpine meadow, Soil bacteria, Co-occurrence network, Soil physicochemical properties

摘要： 牦牛觅食行为是影响青藏高原高寒草甸生态系统的重要因素之一，主要通过采食、践踏和粪便沉积影响土壤生态系统。土壤细菌群落作为土壤生态系统中的重要组成部分，在物质循环和能量流动等方面发挥着关键作用。本文通过在高寒草甸对牦牛进行长期自由放牧处理，分析了不同觅食强度下的细菌群落组成及其共现性网络的变化，揭示了土壤细菌群落对牦牛觅食行为的响应特征。结果表明，细菌群落的优势菌门是变形菌门(Proteobacteria)(30.66%~33.38%)和放线菌门(Actinobacteria)(25.04%~28.99%)，牦牛觅食显著增加了疣微菌门(Verrucomicrobia)(2.46~5.10%)和芽单胞菌门(Gemmatimonadetes)(1.91%~2.68%)的相对丰度。牦牛觅食行为主要通过改变土壤pH值显著影响土壤细菌群落beta多样性，共现性网络复杂度随着觅食强度增加而增加，疣微菌门相对丰度的增加显著降低了细菌群落beta多样性和网络复杂度。牦牛觅食行为能够改善细菌群落稳定性，表明适当的觅食强度能够改善脆弱的高寒草甸生态系统。

关键词: 牦牛觅食, 高寒草甸草原, 土壤细菌, 共现性网络, 土壤理化性质

CLC Number:

S23.8+5

MENG Ling-xu, JIA Cai-ling, XU Chun-xue, ZHANG Xiao-wen, Huhe. Response of Soil Bacterial Community Diversity and Co-occurrence Network to Foraging Intensity of Yaks in Alpine Meadow[J]. Acta Agrestia Sinica, 2023, 31(1): 9-18.

孟令旭, 贾彩玲, 许春雪, 张潇文, 呼和. 高寒草甸土壤细菌群落多样性和共现性网络对牦牛觅食强度的响应[J]. 草地学报, 2023, 31(1): 9-18.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2023.01.002

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2023/V31/I1/9

References

[1] BARDGETT R D,VAN DER PUTTEN W H. Belowground Biodiversity and Ecosystem Functioning[J]. Nature,2014,515(7528):505-511
[2] PELLEGRINI M,PAGNANI G,BERNARDI M,et al. Cell-Free Supernatants of Plant Growth-Promoting Bacteria:A Review of Their Use As Biostimulant and Microbial Biocontrol Agents in Sustainable Agriculture[J]. Sustainablity,2020,12(23):9917
[3] 蒋婧,宋明华. 植物与土壤微生物在调控生态系统养分循环中的作用[J]. 植物生态学报,2010,34(8):979-988
[4] KOHLER F,HAMELIN J,GILLET F,et al. Soil Microbial Community Changes in Wooded Mountain Pastures due to Simulated Effects of Cattle Grazing[J]. Plant and Soil,2005,278(1-2):327-340
[5] GUITIAN R,BARDGETT R D. Plant and Soil Microbial Responses to Defoliation in Temperate Semi-Natural Grassland[J]. Plant and Soil,2000,220:271-277
[6] 王震洪,段昌群,侯永平,等. 植物多样性与生态系统土壤保持功能关系及其生态学意义[J]. 植物生态学报,2006(3):392-403
[7] 周睿,宋梅玲,王玉琴,等. 不同放牧方式下防除黄帚橐吾对高寒草地植物群落的影响[J]. 草地学报,2022,30(7):1819-1828
[8] ZHANG R,WANG Z,NIU S,et al. Diversity of Plant and Soil Microbes Mediates the Response of Ecosystem Multifunctionality to Grazing Disturbance[J]. Science of The Total Environment,2021,776:145730
[9] ZHANG R,WANG Z,HAN G,et al. Grazing Induced Changes in Plant Diversity is a Critical Factor Controlling Grassland Productivity in the Desert Steppe,Northern China[J]. Agriculture,Ecosystems & Environment,2018,265:73-83
[10] RHODES A C,RUTLEDGE J,DUPONT B,et al. Targeted Grazing of an Invasive Grass Improves Outcomes for Native Plant Communities and Wildlife Habitat[J]. Rangeland Ecology & Management,2021,75(1):41-50
[11] WU J,LI M,FIEDLER S,et al. Impacts of Grazing Exclusion on Productivity Partitioning Along Regional Plant Diversity and Climatic Gradients in Tibetan Alpine Grasslands[J]. Journal of Environmental Management,2019,231:635-645
[12] LURGI M,THOMAS T,WEMHEUER B,et al. Modularity and Predicted Functions of the Global Sponge-Microbiome Network[J]. Nature Communication,2019,10(1):992
[13] FAN X,FU Y,NIE Y,et al. Keystone Taxa-Mediated Bacteriome Response Shapes the Resilience of The Paddy Ecosystem to Fungicide Triadimefon Contamination[J]. Journal of Hazardous Materials,2021,417:126061
[14] WANG Y,HONG Y,TIAN Y,et al. Changes in Bacterial Community Composition and Soil Properties Altered the Response of Soil Respiration to Rain Addition in Desert Biological Soil Crusts[J]. Geoderma,2022,409:115635
[15] XUN W,LIU Y,LI W,et al. Specialized Metabolic Functions of Keystone Taxa Sustain Soil Microbiome Stability[J]. Microbiome,2021,9(1):35
[16] WANG X,BIAN Q,JIANG Y,et al. Organic Amendments Drive Shifts in Microbial Community Structure and Keystone Taxa Which Increase C Mineralization Across Aggregate Size Classes[J]. Soil Biology and Biochemistry,2021,153:108062
[17] ZHANG Y,WU X,CHEN C,et al. Application of Thifluzamide Alters Microbial Network Structure and Affects Methane Cycle Genes in Rice-Paddy Soil[J]. Science of The Total Environment,2022,838:155769
[18] WAGG C,SCHLAEPPI K,BANERJEE S,et al. Fungal-Bacterial Diversity and Microbiome Complexity Predict Ecosystem Functioning[J]. Nature Communication,2019,10(1):4841
[19] DE VRIES F T,LIIRI M E,BJØ RNLUND L,et al. Land Use Alters the Resistance and Resilience of Soil Food Webs to Drought[J]. Nature Climate Change,2012,2(4):276-280
[20] DE VRIES F T,GRIFFITHS R I,BAILEY M,et al. Soil Bacterial Networks are Less Stable Under Drought Than Fungal Networks[J]. Nature Communication,2018,9:3033
[21] 邱丽丽,李丹丹,张佳宝,等. 基于共现网络的关键微生物对秸秆还田土壤小麦产量的影响[J]. 土壤学报,2021:1-13
[22] YANG Y,WU L,LIN Q,et al. Responses of the Functional Structure of Soil Microbial Community to Livestock Grazing in the Tibetan Alpine Grassland[J]. Global Change Biology,2013,19(2):637-648
[23] 费璇,锁才序,向双,等. 青藏东缘高寒草甸植物群落结构及功能群特征对长期季节放牧的响应[J]. 草地学报,2022,30(8):1954-1963
[24] YU L,LIU S,WANG F,et al. Effects of Agricultural Activities on Energy-Carbon-Water Nexus of the Qinghai-Tibet Plateau[J]. Journal of Cleaner Production,2022,331:129995
[25] 柴林荣,孙义,王宏,等. 牦牛放牧强度对甘南高寒草甸群落特征与牧草品质的影响[J]. 草业科学,2018,35(1):18-26
[26] 申波,马青青,程云湘,等. 不同放牧制度对土壤种子库的影响:以青藏高原东缘高寒草甸为例[J]. 草业科学,2018,35(4):791-799
[27] CHEN L,JIANG Y,LIANG C,et al. Competitive Interaction with Keystone Taxa Induced Negative Priming Under Biochar Amendments[J]. Microbiome,2019,7:77
[28] MENG L,XU C,WU F,et al. Microbial Co-Occurrence Networks Driven by Low-Abundance Microbial Taxa During Composting Dominate Lignocellulose Degradation[J]. Science of The Total Environment,2022,845:157197
[29] HUANG R,CROWTHER T W,SUI Y,et al. High Stability and Metabolic Capacity of Bacterial Community Promote the Rapid Reduction of Easily Decomposing Carbon in Soil[J]. Communications Biology,2021,4(1):1376
[30] COBAN O,DE DEYN G B,VAN DER PLOEG M. Soil Microbiota As Game-Changers in Restoration of Degraded Lands[J]. Science,2022,375(6584):abe0725
[31] HUHE,CHEN X,HOU F,et al. Bacterial and Fungal Community Structures in Loess Plateau Grasslands with Different Grazing Intensities[J]. Frontiers in Microbiology,2017,8:606
[32] 侯扶江,任继周. 甘肃马鹿冬季放牧践踏作用及其对土壤理化性质影响的评价[J]. 生态学报,2003,23(3):486-495
[33] 任强,艾鷖,胡健,等. 不同强度牦牛放牧对青藏高原高寒草地土壤和植物生物量的影响[J]. 生态学报,2021,41(17):6862-6870
[34] 邵建翔,刘育红,马辉,等. 退化高寒草地浅层土壤理化性质Meta分析[J]. 草地学报,2022,30(6):1370-1378
[35] 郑佳华,赵萌莉,王琪,等. 利用方式对大针茅草原土壤微生物群落结构及多样性的影响[J]. 生态学报,2022(12):1-11
[36] MAESTRE F T,DELGADO-BAQUERIZO M,JEFFRIES T C,et al. Increasing Aridity Reduces Soil Microbial Diversity and Abundance in Global Drylands[J]. Proceedings of the National Academy of Sciences,2015,112(51):15684-15689
[37] SOKOL N W,SLESSAREV E,MARSCHMANN G L,et al. Life and Death in the Soil Microbiome:How Ecological Processes Influence Biogeochemistry[J]. Nature Reviews Microbiology,2022,20(7):415-430
[38] 王永宏,田黎明,艾鷖,等. 短期牦牛放牧强度对川西北高原高寒草甸土壤细菌群落的影响[J]. 生态学报,2022,42(4):1549-1559
[39] 李宏,张青青,江康威,等. 山地草甸不同放牧强度对土壤细菌群落特征的影响[J]. 中国草地学报,2021,43(11):37-44
[40] VESELÁ A B,FRANC M,PELANTOVÁ H,et al. Hydrolysis of Benzonitrile Herbicides by Soil Actinobacteria and Metabolite Toxicity[J]. Biodegradation,2010,21(5):761-770
[41] HARANTOVÁ L,MUDRÁK O,KOHOUT P,et al. Development of Microbial Community During Primary Succession in Areas Degraded by Mining Activities[J]. Land Degradation & Development,2017,28(8):2574-2584
[42] SALLES,FALCAO J,MALLON,et al. Microbial Invasions:The Process,Patterns,and Mechanisms[J]. Trends in Microbiology,2015,23(11):719-729
[43] BARBERAN A,BATES S T,CASAMAYOR E O,et al. Using Network Analysis to Explore Co-Occurrence Patterns in Soil Microbial Communities[J]. The ISME Journal,2012,6(2):343-351
[44] 杜子银,蔡延江,张斌,等. 牲畜排泄物返还对草地土壤氮转化和氧化亚氮(N₂O)排放的影响研究进展[J]. 生态学报,2022,42(1):45-57
[45] 吴宪,胡菏,王蕊,等. 化肥减量和有机替代对潮土微生物群落分子生态网络的影响[J]. 土壤学报,2021,59(2):545-556
[46] XUN W,HUANG T,LI W,et al. Alteration of Soil Bacterial Interaction Networks Driven by Different Long-Term Fertilization Management Practices in the Red Soil of South China[J]. Applied Soil Ecology,2017,120:128-134
[47] 马垒,赵文慧,郭志彬,等. 长期不同磷肥施用量对砂姜黑土真菌多样性、群落组成和种间关系的影响[J]. 生态学报,2019,39(11):4158-4167
[48] STEVENS R J Q U,LAUGHLIN R J. Measurement of Nitrous Oxide and Di-Nitrogen Emissions from Agricultural Soils[J]. Nutrient Cycling in Agroecosystems,1998,52(2-3):131-139
[49] LIU X,YANG T,SHI Y,et al. Strong Partitioning of Soil Bacterial Community Composition and Co-Occurrence Networks Along a Small-Scale Elevational Gradient on Zijin Mountain[J]. Soil Ecology Letters,2021,3(4):290-302
[50] 曲艳,宋倩,杨合龙,等. 呼伦贝尔草原不同利用方式对土壤微生物群落结构的影响[J]. 草地学报,2021,29(8):1621-1627

Response of Soil Bacterial Community Diversity and Co-occurrence Network to Foraging Intensity of Yaks in Alpine Meadow

高寒草甸土壤细菌群落多样性和共现性网络对牦牛觅食强度的响应

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	DU Chen-lu, QIAO Yi-nuo, ZHAO Li-rong, CHEN Han-wen, CAO Meng-yang, LIU Yu. The Characteristics of Root-Soil Complex in Patches of Alpine Meadow [J]. Acta Agrestia Sinica, 2023, 31(1): 202-209.
[2]	LI Lin-zhi, MA Yuan, ZHANG Xiao-yan, HAI Long, LIN Dong, ZHANG De-gang, ZHANG Hai-tao. Distribution Characteristics of Soil Aggregates and its Organic Carbon with Different Degradation Degrees in Alpine Meadow [J]. Acta Agrestia Sinica, 2023, 31(1): 210-219.
[3]	WANG Yu-qin, SONG Mei-ling, ZHOU Rui, WANG Hong-sheng, MA Yu-shou. Characteristics of Plant Community Structure of Different Ligularia virgaurea Density Patches in Alpine Meadow [J]. Acta Agrestia Sinica, 2022, 30(9): 2264-2272.
[4]	LI Ya-nan, LI Shao-yu, SUN Yu, HU Xue-feng, HE Qi-shen, ZHANG Bin, ZHAO Meng-li. Responses of Soil Enzyme Activities and Stoichiometry Characteristics of Desert Steep to Different Stocking Rates [J]. Acta Agrestia Sinica, 2022, 30(8): 2019-2026.
[5]	MA Kai-kai, XU Chang-lin, LI Ying, WEI Kong-tao, BAI Mei-mei, LIU Yuan-yuan, YU Xiao-jun. Effects of Different Rest Grazing Periods on Soil Seed Bank in Cold Season Pasture of Alpine Meadow [J]. Acta Agrestia Sinica, 2022, 30(8): 2100-2107.
[6]	CHAI Yu, LI Xi-lai, YU Jin-feng, YIXI Droma, SONG Xian, MA Pan-pan, DUAN Cheng-wei, XU Wen-yin. Effects of Different Applications of Organic Fertilizer in Degraded Alpine Meadow on Soil Aggregates and Organic Carbon in the Source Zone of Yellow River [J]. Acta Agrestia Sinica, 2022, 30(7): 1613-1620.
[7]	SONG Zhi-ping, WANG Xiao-li, WANG Yan-long, ZHOU Xuan-bo, XIE Le-le. Response of Ecological Niche of Alpine Meadow Population to Rest Grazing Time During Green Period in Three-River Source Region [J]. Acta Agrestia Sinica, 2022, 30(7): 1651-1658.
[8]	HUANG Rui-ling, WANG Xi-wen, MA Guo-hu, ZHU Jin-fu, ZHOU Hua-kun. Effects of Simulated Nitrogen Deposition on Soil Physicochemical Properties and Enzyme Activities in Alpine Wetland [J]. Acta Agrestia Sinica, 2022, 30(6): 1343-1349.
[9]	ZHANG Chun-hui, MA Zhen, REN Yan-mei, WU Lin, YAO Bu-qing, HUANG Xiao-tao, MA Li, GUO Jing, ZHOU Hua-kun. The Study on Drought Tolerance of Six Forbs during Seed Germination in an Alpine Meadow of Qinghai-Tibet Plateau [J]. Acta Agrestia Sinica, 2022, 30(5): 1159-1164.
[10]	ZHANG Cai-jun, WANG Xiao-yan, LIU Qian-qian, AN Kang, PU Qiang-sheng, WANG Zhi-cheng, SU Jun-hu. Effects of Human Activities on the Feeding Habits of Himalayan Marmots in Gannan Meadow [J]. Acta Agrestia Sinica, 2022, 30(5): 1219-1226.
[11]	ZAN Kan-zhuo, LIU Cong, ZHANG Yan-yan, Ashanjiang·Yiminijiang, BAI Jie, LONG Ming-xiu, WANG Ke-zhen, QU Yang, HE Shu-bin. Study on Soil Physical and Chemical Properties of Alfalfa,Wheat and Maize Rhizosphere in Guanzhong Area [J]. Acta Agrestia Sinica, 2022, 30(5): 1246-1252.
[12]	ZHANG Yu-peng, WU Xiao-tian, LI Xi-lai, XU Wen-yin, DONG Xin-pu, WANG Yuan, ZHANG Hui. Responses and Distributions of Degraded Alpine Meadow on the Physical and Chemical Properties of Soil in River Basin Units in the Source Region of Yellow River [J]. Acta Agrestia Sinica, 2022, 30(3): 503-512.
[13]	WANG Fang, LI Wei, LIU Xin, LI Wen-chen, ZHAO Jin-bo, ZHANG Zhi-hui, YANG Zhao. Bacteria Communities of Medicago sativa Rhizosphere Soil in Response to Composted Cow Manure [J]. Acta Agrestia Sinica, 2022, 30(3): 603-611.
[14]	FANG Hao-tian, WENG Bai-sha, CHANG Wen-juan, YANG Yu-heng, GONG Xiao-yan. Characteristics of Root Distribution in Alpine Meadows and the Relationship with Soil Temperature and Moisture [J]. Acta Agrestia Sinica, 2022, 30(3): 612-621.
[15]	WANG Xiao-yan, ZHANG Cai-jun, PU Qiang-sheng, AN Kang, ZHANG Qian, YANG Jing, SUN Xiao-mei, SU Jun-hu. Effects of Artificial Grassland Construction on Grassland Productivity and Soil Physicochemical Properties in Alpine Meadow of Gannan [J]. Acta Agrestia Sinica, 2022, 30(2): 288-296.