Genetic Diversity and Structure Analysis of Germplasm Resources Based on ISSR Markers in Astragalus sinicus L.

doi:10.11733/j.issn.1007-0435.2023.01.006

Acta Agrestia Sinica ›› 2023, Vol. 31 ›› Issue (1): 50-60.DOI: 10.11733/j.issn.1007-0435.2023.01.006

Genetic Diversity and Structure Analysis of Germplasm Resources Based on ISSR Markers in Astragalus sinicus L.

ZHANG Meng^1,3, SHI Peng-fei², LIU Chun-zeng¹, LI Ben-yin¹, DING Li², ZHANG Lin², ZHENG Chun-feng¹, ZHANG Cheng-lan¹, HE Chun-mei⁴, SHI Hai-fan⁵, LYU Yu-hu², CHANG Dan-na⁶, CAO Wei-dong⁶

1. Institute of Plant Nutrition, Agricultural Resource and Environmental Science, Henan Academy of Agricultural Sciences, Zhengzhou, Henan Province 450002, China;
2. Xinyang Academy of Agricultural Sciences, Xinyang, Henan Province 464000, China;
3. Institute of Crops Molecule Breeding, Henan Academy of Agricultural Sciences, Zhengzhou, Henan Province 450002, China;
4. Institute of Soil and Fertilizer, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian Province 350013, China;
5. College of Grassland Science, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China;
6. Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received:2022-09-04 Revised:2022-10-17 Online:2023-01-15 Published:2023-02-07

基于ISSR分子标记的紫云英种质资源遗传多样性及结构分析

张梦^1,3, 史鹏飞², 刘春增¹, 李本银¹, 丁丽², 张琳², 郑春风¹, 张成兰¹, 何春梅⁴, 施海帆⁵, 吕玉虎², 常单娜⁶, 曹卫东⁶

1. 河南省农业科学院植物营养与资源环境研究所, 河南郑州 450002;
2. 信阳市农业科学院, 河南信阳 464000;
3. 河南省作物分子育种研究院, 河南郑州 450002;
4. 福建省农业科学院土壤肥料研究所, 福建福州 350013;
5. 南京农业大学草业学院, 江苏南京 210095;
6. 中国农业科学院农业资源与农业区划研究所, 北京 100081

通讯作者: 刘春增, E-mail:liucz321@aliyun.com;曹卫东, E-mail:caoweidong@caas.cn
作者简介:张梦(1986-)，男，汉族，河南省沁阳人，助理研究员，博士，主要从事绿肥种质资源评价与扩繁研究，E-mail：458945208@qq.com
基金资助:
财政部和农业农村部国家绿肥产业技术体系(CARS-22)；河南省农业科学院自主创新项目(2022ZC74)资助

Abstract

Abstract: Astragalus sinicus L. is one of the most important green manure crops in China. In this study,the genetic diversity and structure of 77 A. sinicus germplasm lines were investigated based on ISSR molecular markers. The results showed that the genetic similarity coefficients of the 77 lines ranged from 0.567 7 to 0.868 6,which had been divided into 6 clusters. The population differentiation score (PhiPT) across the regions was 0.1363,at a significant level (P<0.001),among which the inter-population and intra-population variations accounted for 13.9% and 86.1% respectively. The primer pairs showed various abilities to identify these 77 lines,and ISSR fingerprinting was constructed based on amplification bands 3,8,9,10,12,and 15 by primer pairs of P809 and amplification bands 1,3,5,9,10,and 11 by primer pairs of P886,which could effectively distinguish all the 77 lines. It was discovered that the genetic structure of A. sinicus L. exhibited two subpopulations,one located in the northern regions including Henan,Anhui,Jiangsu and Shanghai with similar genetic structure,and the other in the southwest including Sichuan,Guangxi,Hunan and Jiangxi. The lines in Fujian were with mixed genetic background. It provided a basis for the utilization of resources of A. sinicus L in genetic studies and breeding.

Key words: Astragalus sinicus L., Germplasmresources, Geneticrelationship, Extraction of marker characteristics

摘要： 紫云英(Astragalus sinicus L.)是我国重要的稻田绿肥作物，为揭示不同地区种质资源遗传特征，本研究利用ISSR分子标记对77份紫云英种质资源进行了遗传多样性及结构分析。研究发现，77份供试紫云英种质资源遗传相似性系数为 0.567 7~0.868 6，可划分为6个聚类。不同地区群体间分化系数为 0.136 3，达到极显著水平(P<0.001)，其中种群间变异占比13.9%，种群内变异占比86.1%。不同ISSR引物扩增信息鉴别紫云英资源的能力存在差异，构建了以P809第3，8，9，10，12，15扩增位点以及P886第1，3，5，9，10，11扩增位点为特征的指纹图谱，能有效区分77份供试紫云英资源。以资源所在省份为单元进行群体分析，供试紫云英资源遗传结构呈现分化特征，河南-安徽-江苏-上海资源遗传结构相似，构成北方亚群；四川-广西-湖南-江西资源相似，构成西南方亚群；福建种质资源则呈现混合型特征。本研究结果可为我国紫云英种质资源的科学利用及新品种选育提供依据。

关键词: 紫云英, 种质资源, 遗传关系, 标记特征提取

CLC Number:

S543.9

ZHANG Meng, SHI Peng-fei, LIU Chun-zeng, LI Ben-yin, DING Li, ZHANG Lin, ZHENG Chun-feng, ZHANG Cheng-lan, HE Chun-mei, SHI Hai-fan, LYU Yu-hu, CHANG Dan-na, CAO Wei-dong. Genetic Diversity and Structure Analysis of Germplasm Resources Based on ISSR Markers in Astragalus sinicus L.[J]. Acta Agrestia Sinica, 2023, 31(1): 50-60.

张梦, 史鹏飞, 刘春增, 李本银, 丁丽, 张琳, 郑春风, 张成兰, 何春梅, 施海帆, 吕玉虎, 常单娜, 曹卫东. 基于ISSR分子标记的紫云英种质资源遗传多样性及结构分析[J]. 草地学报, 2023, 31(1): 50-60.

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Genetic Diversity and Structure Analysis of Germplasm Resources Based on ISSR Markers in Astragalus sinicus L.

基于ISSR分子标记的紫云英种质资源遗传多样性及结构分析

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