Acta Agrestia Sinica ›› 2023, Vol. 31 ›› Issue (1): 140-147.DOI: 10.11733/j.issn.1007-0435.2023.01.016

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Effects of the Seed Soaking with DA-6 on Germination Characteristics and Stress Tolerance of White Clover under Salt Stress

CAO Yi-qin, CHEN Bi-zhen, LI Zhou   

  1. College of Grassland Science, Sichuan Agricultural University, Chengdu, Sichuan Province, 611130, China
  • Received:2022-05-20 Revised:2022-10-08 Online:2023-01-15 Published:2023-02-07

胺鲜酯(DA-6)浸种对盐胁迫下白三叶种子萌发及抗盐性的影响

曹亦芹, 程碧真, 李州   

  1. 四川农业大学草业科技学院, 四川 成都 611130
  • 通讯作者: 李州, E-mail:lizhou1986814@163.com
  • 作者简介:曹亦芹(1998-),女,民族,山西太原人,硕士研究生,主要研究方向为植物逆境生理,E-mail:caoyiqin429@163.com
  • 基金资助:
    四川省科技创新(苗子工程)培育项目(2021064)资助

Abstract: Effects of 0.8 mmol·L-1 diethyl aminoethyl hexanote (DA-6) treatment on seeds germination of white clover (Trifolium repens L. 'Haifa’) were investigated under salt stress. One hundred mmol·L-1 NaCl was used as a salt stress treatment to provide a reference for the cultivation and management of turfgrass in urban greenery. Seeds soaking with DA-6 significantly increased the germination vigor,germination percentage,germination index,vitality index,stem length and fresh/dry weight of seedlings (P<0.05). Salt stress significantly induced the accumulation of free proline and soluble sugar,followed by a significantly lower cellular osmotic potential (P<0.05). However,pretreatment with DA-6 immersion under salt stress had no significant effect on the accumulation of free proline and soluble sugar in seedlings (P>0.05). In addition,salt stress led to a significant increase in the content of reactive oxygen species (superoxide anion and hydrogen peroxide),a membrane lipid peroxidation product malondialdehyde,and electrolyte leakage in seedlings (P<0.05). Our current study indicated that DA-6 soaking improved germination characteristics and stress tolerance of white clover seeds by enhancing antioxidant defense and reducing cellular oxidative damage under salt stress.

Key words: White clover, Diethyl aminoethyl hexanote, Salt stress, Osmoregulation, Antioxidant defense

摘要: 以白三叶(Trifolium repens L. 'Haifa’)为研究对象,研究了0.8 mmol·L-1 胺鲜酯(DA-6)浸种处理对白三叶种子在盐胁迫条件下萌发的影响,并对DA-6诱导的耐盐机理进行初步研究,为白三叶的栽培管理提供参考。结果表明,DA-6浸种显著提高了盐胁迫下种子的发芽势、发芽率、活力指数、发芽指数、茎长和鲜干重(P<0.05)。盐胁迫显著诱导了游离脯氨酸和可溶性糖的积累,降低了细胞渗透势(P<0.05)。此外,盐胁迫导致了萌发种子中活性氧(超氧阴离子和过氧化氢)和膜脂过氧化产物丙二醛含量显著增加,电导率显著升高(P<0.05)。DA-6浸种前处理显著提高了胁迫下抗氧化酶(SOD、POD和APX)的活性,增幅分别为33.0%、31.9%和49.1%,这有效地缓解了由盐胁迫引起的氧化损伤,有利于幼苗稳定细胞膜(P<0.05)。本研究表明DA-6浸种处理通过增强抗氧化防御并降低细胞氧化伤害,从而提高盐胁迫下白三叶种子的萌发特性和胁迫耐受性。

关键词: 白三叶, DA-6, 盐胁迫, 渗透调节, 抗氧化防御

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