Research Progress on Nitrite Transformation Pathway and its Influencing Factors in Silage

doi:10.11733/j.issn.1007-0435.2023.04.002

Acta Agrestia Sinica ›› 2023, Vol. 31 ›› Issue (4): 943-951.DOI: 10.11733/j.issn.1007-0435.2023.04.002

Research Progress on Nitrite Transformation Pathway and its Influencing Factors in Silage

AN Jiang-bo¹, LIU Ming-jian¹, LIU Wei¹, BAI Bao-chao¹, JING Zhi-wei², WANG Rui-feng², WANG Zhi-jun¹, GE Gen-tu¹, JIA Yu-shan¹

1. Colleg of Grassland Resources and Environment, Inner Mongolia Agricultural University/Key Laboratory of Forage Cultivation Processing and High Efficient Utilization, Ministry of Agriculture, Hohhot, Inner Mongolia 010019, China;
2. Inner Mongolia Yihe Lvjin Agricultural Development Co., Ltd., Chifeng, Inner Mongolia 025550, China

Received:2022-10-20 Revised:2022-11-29 Published:2023-04-28

青贮过程中亚硝酸盐转化途径及影响因素研究进展

安江波¹, 刘明健¹, 刘伟¹, 白宝超¹, 井志伟², 王瑞峰², 王志军¹, 格根图¹, 贾玉山¹

1. 内蒙古农业大学草原与资源环境学院, 农业农村部饲草栽培、加工与高效利用重点实验室/草地资源教育部重点实验室, 内蒙古呼和浩特 010019;
2. 内蒙古伊禾绿锦农业发展有限公司, 内蒙古赤峰 025550

通讯作者: 贾玉山,E-mail:jys_nm@sina.com
作者简介:安江波(1998-),男,汉族,内蒙古呼和浩特人,硕士研究生,主要从事饲草加工与贮藏研究,E-mail:an474608345@163.com
基金资助:
“国家乳业技术创新中心创建”重点项目(2021-国家乳创中心-1)；内蒙古自治区科技计划项目(2021GG0109)资助

Abstract

Abstract: Silage plays a very important role in the process of animal husbandry production,but the accumulation of nitrite during silage fermentation is seriously harmful to animal production performance,milk and meat quality. Nitrite poisoning is a common foodborne disease to livestock. Mild poisoning can affect the growth and development of livestock,severe poisoning can even lead to the death of livestock. To reduce the risk of nitrite in silage and improve the quality of silage and secure the safety of silage,this paper described the hazards,sources,transformation pathways,nitrite degradation pathways and control measures during the silage process,to provide an effective reference for nitrite degradation and regulation in silage feed.

Key words: Silage, Nitrite, Transformation pathways, Degradation pathway, Control measures

摘要： 青贮饲料在畜牧业生产过程中占有非常重要的地位，但是青贮发酵过程中亚硝酸盐积累会严重危害动物生产性能及奶肉品质。亚硝酸盐中毒是家畜在临床上常见的一种食源性的疾病，家畜轻度中毒会影响其生长发育，重度中毒甚至会导致家畜死亡。因此，为减少家畜因食用青贮引发亚硝酸盐中毒的现象，同时提高青贮质量安全，本文阐述了青贮亚硝酸盐的危害、来源、转化途径、青贮过程中亚硝酸盐降解途径及调控措施，以期为青贮中亚硝酸盐降解及调控提供有效参考。

关键词: 青贮, 亚硝酸盐, 转化途径, 降解途径, 调控措施

CLC Number:

S816.5+3

AN Jiang-bo, LIU Ming-jian, LIU Wei, BAI Bao-chao, JING Zhi-wei, WANG Rui-feng, WANG Zhi-jun, GE Gen-tu, JIA Yu-shan. Research Progress on Nitrite Transformation Pathway and its Influencing Factors in Silage[J]. Acta Agrestia Sinica, 2023, 31(4): 943-951.

安江波, 刘明健, 刘伟, 白宝超, 井志伟, 王瑞峰, 王志军, 格根图, 贾玉山. 青贮过程中亚硝酸盐转化途径及影响因素研究进展[J]. 草地学报, 2023, 31(4): 943-951.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2023.04.002

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2023/V31/I4/943

References

[1] 邱孝煊,黄东风,蔡顺香,等. 施肥对蔬菜硝酸盐累积的影响研究[J]. 中国生态农业学报,2004,12(2):116-119
[2] 贾玉山,于浩然,都帅,等. 天然牧草青贮添加剂研究进展[J]. 草地学报,2018,26(3):533-538
[3] 王晓娜. 蒙晋津京青贮饲料质量与安全性评价[D]. 北京:中国农业科学院,2011:11
[4] 中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会. GB 13078-2017,饲料卫生标准[S]. 北京:中国标准出版社,2017:8
[5] 王春林. 饲料中硝酸盐及亚硝酸盐中毒问题[J]. 黑龙江畜牧兽医,2000(9):19-20
[6] BRADLEY W,EPPSON H,BEATH O. Methylne Blue as an Antidote for Poisoning by Oat Hay and Other Plants Containing Nitrates[J]. Journal of the American Veterinary Medical Association,1940,96(754):41-42
[7] VEGA-VILLA K,PLUTA R,LONSER R,et al. Quantitative Systems Pharmacology Model of NO Metabolome and Methemoglobin Following Long-term Infusion of Sodium Nitrite in Humans[J]. CPT:Pharmacometrics and Systems Pharmacology,2013,2(7):1-10
[8] 马玉胜. 反刍动物亚硝酸盐中毒的原因与防治措施[J]. 湖北畜牧兽医,2006(2):30
[9] SCHRENK D,BIGNAMI M,BODIN L,et al. Risk Assessment of Nitrate and Nitrite in Feed[J]. EFSA Journal,2020,18(11):e06290
[10] LIN K,SHEN W,SHEN Z,et al. Estimation of the Potential for Nitrosation and Its Inhibition in Subjects from High-and Low-Risk Areas for Esophageal Cancer in Southern China[J]. International Journal of Cancer,2003,107(6):891-895
[11] 李智文,张乐,王丽娜. 硝酸盐、亚硝酸盐及N-亚硝基化合物与人类先天畸形[J]. 环境与健康杂志,2005(6):92-94
[12] 宗亚倩,韩博,段新慧,等. 混合青贮微生物多样性研究进展[J]. 草地学报,2022,30(10):2535-2540
[13] 张鸣珠,黄媛,吴长荣,等. 青贮原料表面微生物研究进展[J]. 中国饲料,2022(19):1-8
[14] CANFIELD D E,GLAZER A N,FALKOWSKI P G. The Evolution and Future of Earth's Nitrogen Cycle[J]. Science,2010,330(6001):192-196
[15] KNOWLES R. Denitrification[J]. Microbiological Reviews,1982,46(1):43-70
[16] ZUMFT W G. Cell Biology and Molecular Basis of Denitrification[J]. Microbiology and Molecular Biology Reviews,1997,61(4):533-616
[17] PHILIPPOT L. Denitrifying Genes in Bacterial and Archaeal Genomes[J]. Biochimica et Biophysica Acta (BBA)-Gene Structure and Expression,2002,1577(3):355-376
[18] TANIMOTO T,HATANO K I,KIM D H,et al. Co-Denitrification by the Denitrifying System of the Fungus Fusarium Oxysporum-ScienceDirect[J]. FEMS Microbiology Letters,1992,93(2):177-180
[19] JU X,XING L,GAO Z,et al. Processes and Factors Controlling N₂O Production in an Intensively Managed Low Carbon Calcareous Soil Under Sub-Humid Monsoon Conditions[J]. Environmental Pollution,2011,159(4):1007-1016
[20] ZHANG S,QIN W,BAI Y,et al. Linkages Between Anammox and Denitrifying Bacterial Communities and Nitrogen Loss Rates in High-elevation Rivers[J]. Limnology and Oceanography,2020,66(3):1-14
[21] 钟磊,王一喆,李鸣. 草地硝化和反硝化微生物功能群研究进展[J]. 中国农学通报,2018,34(3):6
[22] XIA S,LI J,WANG R,et al. Tracking Composition and Dynamics of Nitrification and Denitrification Microbial Community in a Biofilm Reactor by PCR-DGGE and Combining FISH with Flow Cytometry[J]. Biochemical Engineering Journal,2010,49(3):370-378
[23] BRAKER G,ZHOU J,WU L,et al. Nitrite Reductase Genes (nirK and nirS) as Functional Markers to Investigate Diversity of Denitrifying Bacteria in Pacific Northwest Marine Sediment Communities[J]. Applied and Environmental Microbiology,2000,66(05):2096-2104
[24] 董莲华,杨金水,袁红莉. 氨氧化细菌的分子生态学研究进展[J]. 应用生态学报,2008,19(6):8
[25] 冯惠. 基于AHL的群体感应强化受微污染水中微生物去除氨氮及机制研究[D]. 哈尔滨:哈尔滨工业大学,2017:9-13
[26] HOLT J G,KRIEG N R,SNEALTH P,et al. Bergey's Manual of Determinative Bacteriology[M].Baltimore:The Williams and Wilkins Company,1994:447-450
[27] KUENEN J G,ROBERTSON L A. Combined Nitrification-Denitrification Processes[J]. FEMS Microbiology Reviews,1994,15(2-3):109-117
[28] PEDERSEN H,DUNKIN K A,FIRESTONE M K. The Relative Importance of Autotrophic and Heterotrophic Nitrification in a Conifer Forest Soil as Measured by 15 N Tracer and Pool Dilution Techniques[J]. Biogeochemistry,1999,44(2):135-150
[29] MÉVEL G,PRIEUR D. Heterotrophic Nitrification by a Thermophilic Bacillus Species as Influenced by Different Culture Conditions[J]. Canadian Journal of Microbiology,2000,46(5):465-473
[30] KIM J S,KIM S J,LEE B H. Effect of Alcaligenes faecalis on Nitrous Oxide Emission and Nitrogen Removal in Three Phase Fluidized Bed Process[J]. Journal of Environmental Science and Health Part A,2004,39(7):1791-1804
[31] 张光亚,方柏山,闵航,等. 一株好氧反硝化菌的特征及系统进化分析[J]. 华侨大学学报：自然科学版,2004,25(1):4
[32] LIU Y,WANG Y,LI Y,et al. Nitrogen Removal Characteristics of Heterotrophic Nitrification-aerobic Denitrification by Alcaligenes faecalis C16[J]. Chinese Journal of Chemical Engineering,2015,23(5):827-834
[33] PAPEN H,BERG R V. A Most Probable Number Method (MPN) for the Estimation of Cell Numbers of Heterotrophic Nitrifying Bacteria in Soil[J]. Plant and Soil,1998,199(1):123-130
[34] 辛玉峰,曲晓华,袁梦冬,等. 一株异养硝化-反硝化不动杆菌的分离鉴定及脱氮活性[J]. 微生物学报,2011,51(12):1646-1654
[35] ROUT P R,BHUNIA P,DASH R R. Simultaneous Removal of Nitrogen and Phosphorous from Domestic Wastewater Using Bacillus Cereus GS-5 Strain Exhibiting Heterotrophic Nitrification,Aerobic Denitrification and Denitrifying Phosphorous Removal[J]. Bioresource Technology,2017,224(1):484-495
[36] 刘志培,刘双江. 硝化作用微生物的分子生物学研究进展[J]. 应用与环境生物学报,2004,10(4):5
[37] TIEDJE J M,SEXSTONE A J,MYROLD D D,et al. Denitrification:Ecological Niches,Competition and Survival[J]. Antonie Van Leeuwenhoek,1983,48(6):569-583
[38] 卜翠娜. 异化硝酸盐还原菌(DNRA)的环境分布及富集培养研究[D].济南:山东大学,2018:9
[39] KARTAL B,KUYPERS M,LAVIK G,et al. Anammox Bacteria Disguised as Denitrifiers:Nitrate Reduction to Dinitrogen Gas via Nitrite and Ammonium[J]. Environmental Microbiology,2007,9(3):635-642
[40] CHEN Z,DING W,XU Y,et al. Importance of Heterotrophic Nitrification and Dissimilatory Nitrate Reduction to Ammonium in a Cropland Soil:Evidences from a 15 N Tracing Study to Literature Synthesis[J]. Soil Biology and Biochemistry,2015,91:65-75
[41] SCHMIDT C S,RICHARDSON D J,BAGGS E M. Constraining the Conditions Conducive to Dissimilatory Nitrate Reduction to Ammonium in Temperate Arable Soils[J]. Soil Biology and Biochemistry,2011,43(7):1607-1611
[42] 李倩霞. 异化硝酸盐还原菌(DNRA)对不同碳源的适应性及其在自然环境中的分布研究[D]. 济南:山东大学,2020:59-60
[43] FUERST J A,SAGULENKO E. Beyond the Bacterium:Planctomycetes Challenge our Concepts of Microbial Structure and Function[J]. Nature Reviews Microbiology,2011,9(6):403-413
[44] JIA F,PENG Y,LI J,et al. Metagenomic Prediction Analysis of Microbial Aggregation in Anammox-Dominated Community[J]. Water Environment Research,2021,93(11):2549-2558
[45] MURPHY L S,SMITH G E. Nitrate Accumulations in Forage Crops[J]. Agronomy Journal,1967,59(2):171-174
[46] 苏希孟. 青绿饲料的中毒及预防措施[J]. 国外畜牧学(猪与禽),2009,29(1):85-87
[47] 蔡碧玉. 动物硝酸盐与亚硝酸盐中毒及其防治[J]. 福建畜牧兽医,2010,32(6):51-53
[48] 郭丽艳,唐垚,黄润秋,等. 市售酱腌菜中亚硝酸盐含量及影响因素分析[J]. 中国调味品,2022,47(11):164-167
[49] 韩丹蕊,孙启忠. 呼伦贝尔草原牧草青贮饲料硝酸盐与亚硝酸盐含量研究[J]. 草地学报,2014,22(6):1360-1364
[50] 冯鹏,孙启忠. 不同比例玉米与沙打旺混贮营养成分及有毒有害物质分析[J]. 畜牧兽医学报,2011,42(9):1264-1270
[51] 韩立英,玉柱. 施氮肥对全株玉米青贮饲料硝酸盐含量的影响[J]. 中国饲料,2010(20):37-39
[52] 杨晶晶,孙芸,吴庆宇,等. 受风灾倒伏玉米的青贮调制及其发酵品质影响因素[J]. 中国饲料,2022(1):88-97
[53] 李小梅. 多花黑麦草产量品质对氮肥运筹的响应及其微生物发酵机理研究[D]. 雅安:四川农业大学,2019:10-25
[54] 许庆方,玉柱,侯建刚,等. 灌溉对青贮玉米产量和品质的影响[J]. 中国饲料,2019(3):46-49
[55] HUANG T T,WU Z Y,ZHANG W X. Effects of Garlic Addition on Bacterial Communities and the Conversions of Nitrate and Nitrite in a Simulated Pickle Fermentation System[J]. Food Control,2020,113:107215
[56] 倪奎奎. 全株水稻青贮饲料中微生物菌群以及发酵品质分析[D]. 郑州:郑州大学,2016:11-12
[57] 陶莲,刁其玉. 青贮发酵对玉米秸秆品质及菌群构成的影响[J]. 动物营养学报,2016,28(1):198-207
[58] BAI C,PAN G,LENG R,et al. Effect of Ensiling Density and Storage Temperature on Fermentation Quality,Bacterial Community,and Nitrate Concentration of Sorghum-Sudangrass Silage[J]. Frontiers in Microbiology,2022,13:828320
[59] ZHAO M,ZHANG H,PAN G,et al. Effect of Exogenous Microorganisms on the Fermentation Quality,Nitrate Degradation and Bacterial Community of Sorghum-Sudangrass Silage[J]. Frontiers in Microbiology,2022,13:1052837
[60] MASUKO T,侯发远. 青贮期间硝酸盐消失与微生物群落的关系[J]. 四川草原,1983(4):90-96，14
[61] 冯鹏,温定英,孙启忠. 种植密度对玉米产量及青贮品质的影响[J]. 草业科学,2011,28(12):2203-2208
[62] SPOELSTRA S F. Nitrate in Silage[J]. Grass and Forage Science,1985,40(1):1-11
[63] 杨春哲,冉艳红. 乳酸菌在泡菜生产中的应用[J]. 中国食物与营养,2003(1):28-29
[64] 杨洁彬,郭兴华,张篪,等. 乳酸菌:生物学基础及应用[M]. 北京:中国轻工业出版社,1996:109-110
[65] KRESKE A C,BJORNSDOTTIR K,BREIDT JR F,et al. Effects of pH,Dissolved Oxygen,and Ionic Strength on the Survival of Escherichia Coli O157:H7 in Organic Acid Solutions[J]. Journal of Food Protection,2008,71(12):2404-2409
[66] HASHIMOTO T. The Cause on the Abnormal Accumulation of Nitrite in Pickles of Chinese Cabbage (Brassica Pekinesis Rupr.)[J]. Journal of the Japanese Society for Food Science and Technology (Japan),2001,48(6):409-415
[67] 张庆芳,迟乃玉,郑燕,等. 乳酸菌降解亚硝酸盐机理的研究[J]. 食品与发酵工业,2002(8):27-31
[68] LUO Y,LIU Y,REN T,et al. Sichuan Paocai Fermented by Mixed-Starter Culture of Lactic acid Bacteria[J]. Food Science and Nutrition,2020,8(10):5402-5409
[69] YU S M,ZHANG Y. Effects of Lactic Acid Bacteria on Nitrite Degradation during Pickle Fermentation [C]// Advanced Materials Research.Zhangjiajie:Trans Tech Publications Ltd,2013:1656-1660
[70] 吴征敏. 菠萝渣青贮条件优化及对雷州山羊瘤胃体外发酵特性影响的研究[D]. 湛江:广东海洋大学,2019:13
[71] FEI Y T,LIU D M,LUO T H,et al. Molecular Characterization of Lactobacillus Plantarum DMDL 9010,a Strain with Efficient Nitrite Degradation Capacity[J]. PLoS One,2014,9(11):e113792
[72] 沈昳潇,魏宝东. 不同乳酸菌株对大白菜腌渍过程亚硝酸盐含量变化及pH的影响[J]. 北方园艺,2010(22):160-162
[73] YANG H,ZOU H,QU C,et al. Dominant Microorganisms During the Spontaneous Fermentation of Suan Cai,a Chinese Fermented Vegetable[J]. Food Science and Technology Research,2014,20(5):915-926
[74] 马延岩. 发酵蔬菜中亚硝酸盐的生成及降解机理研究[D]. 天津:天津科技大学,2010:45
[75] ROCHA B S,GAGO B,RUI M B,et al. Diffusion of Nitric Oxide Through the Gastric Wall upon Reduction of Nitrite by Red Wine:Physiological Impact[J]. Nitric Oxide,2010,22(3):235-241
[76] BRANDAO G C,LIMA D C,FERREIRA S L C. The Chemical Generation of NO for the Determination of Nitrite by High-Resolution Continuum Source Molecular Absorption Spectrometry[J]. Talanta,2012,98:231-235
[77] DING Z,JOHANNINGSMEIER S D,PRICE R,et al. Evaluation of Nitrate and Nitrite Contents in Pickled Fruit and Vegetable Products[J]. Food Control,2018,90:304-311
[78] 张少颖. 不同处理方法对泡菜发酵过程中亚硝酸盐含量的影响[J]. 中国食品学报,2011,11(1):133-138
[79] 柴政,蔡津津,安思婧,等. 食醋对泡白菜亚硝酸盐含量的影响[J]. 中国调味品,2010,35(9):51-55
[80] 李春,王宝才,刘丽波. 亚硝酸盐降解影响因素的研究[J]. 食品工业,2010,31(4):7-9
[81] 邹辉,刘晓英,陈义伦,等. 泡菜(白菜)腌制过程中有机酸对亚硝酸盐含量的影响![J]. 食品与发酵工业,2013,39(11):29-32
[82] 杨晶. 植物乳杆菌Lactobacillus plantarum 5-7-3降解亚硝酸盐作用的研究[D]. 扬州:扬州大学,2019:38-39
[83] 徐柯,成林林,袁美,等. 柠檬酸对泡菜中硝酸盐、亚硝酸盐及其风味物质的影响[J]. 中国酿造,2019,38(10):77-83
[84] 商景天. 泡菜中降解亚硝酸盐菌种筛选及其降解机理研究[D]. 贵州:贵州大学,2019:46-47
[85] 蔡庆生. 植物生理学[M].北京:中国农业大学出版社,2014:54-55
[86] 丁少南. 植物乳杆菌中亚硝酸还原酶的研究[D]. 上海:上海师范大学,2013:5-9
[87] TIKHONOVA T V,SLUTSKY A, ANTIPOV A N,et al.Molecular and Catalytic Properties of a Novel Cytochrome C Nitrite Reductase from Nitrate-Reducing Haloalkaliphilic Sulfur-Oxidizing Bacterium Thioalkalivibrio Nitratireducens[J]. Biochimica Et Biophysica Acta,2006,1764(4):715-723
[88] MARIA P. Advances in Citric Acid Fermentation by Aspergillus Niger:Biochemical Aspects,Membrane Transport and Modeling[J]. Biotechnology Advances,2007,25(3):244-263
[89] 付豪,邓艳芳,黄丽玲,等. 亚硝酸盐还原酶高产菌株的筛选与产酶条件的研究[J]. 食品研究与开发,2021,42(10):179-185
[90] 刘萍. 亚硝酸还原酶的制备及亚硝酸还原酶制剂的制备方法:中国,CN101230337A[P]. 2008-07-30
[91] SCOTT K P,GRATZ S W,SHERIDAN P O,et al. The Influence of Diet on the Gut Microbiota[J]. Pharmacological Research:The Official Journal of the Italian Pharmacological Society,2013,69(1):52-60
[92] 刘文平,梁烜赫,张春宵,等. 植物氮素利用途径中硝酸盐转运基因的研究进展[J]. 中国土壤与肥料,2022(7):238-246
[93] 刘观忠. 成年奶牛的饲养管理技术[J]. 中国乳业,2019(1):47-53
[94] WRIGHT M J,DAVISON K L. Nitrate Accumulation in Crops and Nitrate Poisoning in Animals[J]. Advances in Agronomy,1964,16:197-247
[95] 杨汝馨,张涵,徐伟健,等. 不同施灌条件蔬菜种植土壤中硝酸盐运移研究[J]. 环境工程,2018,36(7):133-137
[96] 郝俊峰,于浩然,贾玉山,等. 青贮密度和青贮时间对紫花苜蓿发酵品质及营养成分的影响[J]. 草地学报,2022,30(9):2492-2496
[97] 李奕佳,谭兴和,郭红英,等. 食盐含量和原料含水量对发酵芥菜品质的影响[J]. 中国酿造,2016,35(12):35-39
[98] 段娜宁,王伟,魏希杰,等. 不同处理方式对高寒地区燕麦青贮乳酸菌数量及pH值的影响[J]. 青海畜牧兽医杂志,2021,51(6):37-45
[99] 凌洁玉,陈旭,陈思羽,等. 植物乳杆菌降解亚硝酸盐及其亚硝酸盐还原酶的研究[J]. 中国调味品,2017,42(7):72-75,89
[100] LI W T,CHE Z M,XING Y G,et al. Effect of Different Fermentation Conditions on the Nitrite Concentration of Carrot-Pickles Fermented by Lactobacillus Preparation[J]. Advanced Materials Research,2011,338:782-785
[101] 刘玮,邱崇顺,何宇星,等. 一株降解亚硝酸盐植物乳杆菌的生长特性研究及其在泡菜制作中的应用[J]. 现代食品科技,2022,38(9):1-8
[102] 于沛,王修俊,商景天,等. LP-7501S用于发酵萝卜中亚硝酸盐降解工艺的研究[J]. 中国调味品,2020,45(5):63-68
[103] 孙全敏,迟雪梅,马明昊,等. 一种筛选高效降解亚硝酸盐海洋低温细菌的方法[J]. 中国食品添加剂,2022,33(4):129-135

Research Progress on Nitrite Transformation Pathway and its Influencing Factors in Silage

青贮过程中亚硝酸盐转化途径及影响因素研究进展

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	GONG Hai-qiang, CHAI Ji-kuan, CHEN San-dong, YIN Guo-li, DENG Xiu-xia. The Screening and Identification of Lactic Acid Bacteria Against Aspergillus flavus [J]. Acta Agrestia Sinica, 2023, 31(2): 587-595.
[2]	QI He-xing, WANG Hai-chun, LU Guang-xin, LI Zong-ren, TIAN Feng, HE Chen-bang, XU Cheng-ti, ZOU Hai-tao, LEI Sheng-yan, LI Hong-fang, TANG Hai-peng. Isolation,Identification and Pathogenicity Analysis of Helminthosporioid Fungi from Silage Maize [J]. Acta Agrestia Sinica, 2023, 31(1): 40-49.
[3]	LIU Yi-chao, SI Qiang, LIU Ming-jian, LU Xuan, YAN Meng, GE Gen-tu, WANG Zhi-jun, JIA Yu-shan. Effect of Filling Density on Quality and Aerobic Stability of Leymus chinensis and Alfalfa Silage [J]. Acta Agrestia Sinica, 2023, 31(1): 263-271.
[4]	SUN Rong, LIU Yue, CHEN Ting, LYU Ren-long, ZHOU Han-lin, LI Mao, ZI Xue-juan. Effect of Lactic Acid Bacteria and Molasses on the Silage Quality and Bacterial Diversity of Paper Mulberry [J]. Acta Agrestia Sinica, 2023, 31(1): 280-286.
[5]	LI Hai-ping, GUAN Hao, JIA Zhi-feng, LIU Wen-hui, CHEN Shi-yong, XU Mei-ling, CHEN Sheng-cui, YAN Dong-hai, JIANG Yong-mei, GAN Li, ZHANG Jia-min, ZHOU Qing-ping. Effects of Wheat Bran and Lactic Acid Bacteria on Baled Oat Silage Quality and Aerobic Stability in the Alpine Region of Northwest Sichuan [J]. Acta Agrestia Sinica, 2023, 31(1): 302-310.
[6]	QI Shuai, QIN Wei-na, JIAO Ting, SHI Shang-li, GAO Yong-quan, WANG Hu-ning, LI Shu-yan. Evaluation on Physiological Characteristics and Water Use Efficiency for Different Silage Maize Varieties in Gansu Rain-Fed Area [J]. Acta Agrestia Sinica, 2022, 30(9): 2407-2414.
[7]	HAO Jun-feng, YU Hao-ran, JIA Yu-shan, WANG Zhi-jun, GE Gen-tu, LI Yu-yu, SUN Lin. Effects of Ensiling Density and Time on the Fermentation Quality and Nutrient Composition of Alfalfa Silage [J]. Acta Agrestia Sinica, 2022, 30(9): 2492-2496.
[8]	LONG Shi-he, LI Xue-feng, PAN Jun-xin, CHANG Ye, SHI Meng-li, HUANG Nai-xin, CUI Zhi-hai, WANG Jian. Study on the Silage Quality and Microbial Community Diversity of 'Reyan No.4’ King Grass Harvested at Different Growth Days [J]. Acta Agrestia Sinica, 2022, 30(7): 1900-1908.
[9]	ZHANG Shu-xing, WANG Xiao, MA Lin, WU Xin-ming, SHI Yong-hong, WANG Xue-min, ZHAO Wei. Comprehensive Evaluation of Performance and Forage Quality of Silage Maize in Farming-pastoral Ecotone [J]. Acta Agrestia Sinica, 2022, 30(6): 1517-1524.
[10]	XU Liu-xing, WU Dan, ZHANG Jian-guo. Yield, Nutritional Composition, Silage Fermentation Quality of Whole-plant and Their Correlation of Different Wheat Varieties Planted in Guangdong Province [J]. Acta Agrestia Sinica, 2022, 30(6): 1525-1533.
[11]	FU Zhi-hui, GE Gen-tu, JIA Yu-shan, WANG Zhi-jun, SUN Lin, NAN Ding-han, YUE Zheng-shan, YAN Feng, REN Xiu-zhen. Effects of Additives on Silage Quality and Microorganism Counts of Ryegrass [J]. Acta Agrestia Sinica, 2022, 30(5): 1296-1301.
[12]	ZHANG Hai-xuan, HUO Wen-jie, CHEN Lei, LIU Qiang, ZHANG Shuan-lin, WANG Cong, XU Qing-fang, GUO Gang. Effects of Additives on the Fermentation Quality and the Absorption of Amino Acids in the Small Intestine of Alfalfa Silage [J]. Acta Agrestia Sinica, 2022, 30(5): 1302-1309.
[13]	JIA Hong-ding, HUANG Li-juan, LIU Lei, SUN Rong-ji, ZHANG Zhi-fei, CHEN Gui-hua. Comparative Study on the Quality of Rice Straw Silage of Different Varieties [J]. Acta Agrestia Sinica, 2022, 30(5): 1310-1318.
[14]	ZHAO Mu-qier, WANG Zhi-jun, BAO Jian, SUN Lin, ZHANG Yong-hu, WEN Rui, WANG Wei, JIA Yu-shan, GE Gen-tu. Effect of Different Silage Additives on the Silage Quality of Forage Millet [J]. Acta Agrestia Sinica, 2022, 30(4): 1021-1026.
[15]	WANG Li, WANG Chuan-jie, PAN Min, CHENG Zhi-qiang, WEI Yuan-hao, HUA He-liang, YIN Zhi-tong, ZHAO Guo-qi, HUANG Qian-qian. Study on Silage Fermentation Quality and Rumen Degradation Characteristics of Four Silage Maize Varieties [J]. Acta Agrestia Sinica, 2022, 30(3): 568-574.