Effect of Invasion and Diffusion of Poisonous Weeds on Soil Microbial Diversity in Subalpine Meadow in Northwest Yunnan

doi:10.11733/j.issn.1007-0435.2023.07.009

Acta Agrestia Sinica ›› 2023, Vol. 31 ›› Issue (7): 1996-2004.DOI: 10.11733/j.issn.1007-0435.2023.07.009

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Effect of Invasion and Diffusion of Poisonous Weeds on Soil Microbial Diversity in Subalpine Meadow in Northwest Yunnan

NIU Qiong-mei¹, SHAN Gui-lian¹, LUO Qin¹, LIU Yang², LIU Jia³, XIE Yong⁴, DENG Shen-cai¹, YANG Xin¹, CHU Xiao-hui¹

1. Department of Grassland Science, Yunnan Agricultural University, Kunming, Yunnan Province 650201, China;
2. Forestry and Grassland Bureau of Yunnan Province, Kunming, Yunnan Province 650224, China;
3. Shangri La Forestry and Grassland Bureau, Diqing, Yunnan Province 674499, China;
4. Kunming Municipal Animal Disease Prevention and Control Center, Kunming, Yunnan Province 650223, China

Received:2022-12-22 Revised:2023-02-16 Online:2023-07-15 Published:2023-08-01

毒害草入侵扩散对滇西北亚高山草甸土壤微生物多样性的影响

牛琼梅¹, 单贵莲¹, 罗钦¹, 刘洋², 刘佳³, 谢勇⁴, 邓申才¹, 杨欣¹, 初晓辉¹

1. 云南农业大学草业科学系, 云南昆明 650201;
2. 云南省林业与草原局, 云南昆明 650224;
3. 香格里拉市林业和草原局, 云南迪庆 674499;
4. 昆明市动物疫病预防控制中心, 云南昆明 650223

通讯作者: 初晓辉,E-mail:478491637@qq.com
作者简介:牛琼梅(1997-),女,汉族,云南罗平人,硕士研究生,E-mail:2937196663@qq.com;单贵莲(1982-),女,汉族,云南沾益人,教授,博士,E-mail:shanguilian8203@126.com
基金资助:
云南省农业联合专项(202101BD070001-068)；云南省教育厅科学研究基金项目(2023Y0979)；云南农业大学第十六届科技创新创业行动基金项目(2023Y0284)；云南省万人计划青年拔尖人才专项项目资助

Abstract

Abstract: To explore the effect of poisonous weeds invasion on soil microorganisms in subalpine meadow in northwest Yunnan,the fixed monitoring site of shangri-La was selected as the research object,and three patches,which are dominated by Poa pratensis(set as control),Euphorbia jolkinii and Ligularia dictyoneura, were chosen as the sampling units,and the effect of poisonous weeds invasion and diffusion on soil microbial diversity of subalpine meadow was investigated in this study. The results showed as follows:compared with what in Poa pratensis patch,the density of soil bacteria,fungi and actinomycetes,the total OTUs and endemic OTUs,the α diversity and β diversity of soil bacteria and fungi were significantly increased in E. jolkinii patch and L. dictyoneura patch (P<0.05). At the level of bacterial phyla,the relative abundance of Acidobacteriota and Chloroflexi decreased,while that of Proteobacteria increased. At the fungal phylum level,the relative abundance of Basidiomycota decreased,while that of Ascomycota increased. At the genus level,the relative abundance of Archaeorhizomyces decreased,while that of Hygrocybe increased contrarily. In conclusion,the invasion and diffusion of E. jolkinii and L. dictyoneura increased the density of soil microbial flora and the number of OTUs,changed the composition and diversity of microbial species,and promoted the growth and reproduction of soil microorganisms.

Key words: Euphorbia jolkinii, Ligularia dictyoneura, Invasion and diffusion, Soil Microbial

摘要： 为探明毒害草入侵对滇西北亚高山草甸土壤微生物的影响。本研究以香格里拉市国家草原固定监测样地为研究对象，以样地内的草地早熟禾(Poa pratensis)(设为对照)、大狼毒(Euphorbia jolkinii)、网脉橐吾(Ligularia dictyoneura)斑块为取样单元，开展毒害草入侵扩散对亚高山草甸土壤微生物多样性的影响研究。结果表明：与早熟禾斑块相比，大狼毒和网脉橐吾斑块土壤细菌、真菌和放线菌菌群密度，土壤细菌和真菌总OTU数量、特有OTU数量、α多样性和β多样性均显著增加(P＜0.05)。在细菌门水平上，酸杆菌门(Acidobacteriota)、绿弯菌门(Chloroflexi)相对丰度降低，变形菌门(Proteobacteria)相对丰度增加。在真菌门水平上，担子菌门(Basidiomycota)相对丰度降低，子囊菌门(Ascomycota)相对丰度增加。在属水平上，古根菌属(Archaeorhizomyces)相对丰度降低，湿伞属(Hygrocybe)相对丰度增加。综上，大狼毒和网脉橐吾的入侵扩散增加了土壤微生物菌群密度及OTU数量，改变了微生物物种组成及多样性，促进了土壤微生物的生长繁殖。

关键词: 大狼毒, 网脉橐吾, 入侵扩散, 土壤微生物

CLC Number:

S154.3

NIU Qiong-mei, SHAN Gui-lian, LUO Qin, LIU Yang, LIU Jia, XIE Yong, DENG Shen-cai, YANG Xin, CHU Xiao-hui. Effect of Invasion and Diffusion of Poisonous Weeds on Soil Microbial Diversity in Subalpine Meadow in Northwest Yunnan[J]. Acta Agrestia Sinica, 2023, 31(7): 1996-2004.

牛琼梅, 单贵莲, 罗钦, 刘洋, 刘佳, 谢勇, 邓申才, 杨欣, 初晓辉. 毒害草入侵扩散对滇西北亚高山草甸土壤微生物多样性的影响[J]. 草地学报, 2023, 31(7): 1996-2004.

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Effect of Invasion and Diffusion of Poisonous Weeds on Soil Microbial Diversity in Subalpine Meadow in Northwest Yunnan

毒害草入侵扩散对滇西北亚高山草甸土壤微生物多样性的影响

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