Abstract: To clarify the effects of bacterial secondary symbionts on the biological characteristics of sexual generations of Acyrthosiphon pisum red morph,A. pisum red morph infected with both Hamiltonella and Regiella were used as initial aphids. First,clones uninfected with bacterial secondary symbionts were obtained using the antibiotic feeding method,then 3 artificially infected clones were prepared. Each infected clone was placed under a short daylight environment to reveal the sex ratio and then 9 different mating treatments all of which were sexual aphids were prepared and after mating the longevity of the sexual aphids,reproduction and fertilisation success rate of each mating treatment were counted,and the incubation period and hatching rate of fertilised eggs were further investigated. The results showed that the sex ratio becomes female biased and the longevity of sexual aphids reduced when infected with Hamiltonella or Regiella. Infection of oviparous females reduced the number of eggs,while infection of males resulted in reduced fertilisation rates. Whether the fertilised eggs infected with bacterial secondary symbionts or not had no significant effect on the hatching calendar,however,the hatching rate of fertilised eggs infected with bacterial secondary symbionts was significantly higher than that of uninfected eggs. There was no additive effect after simultaneous infection with Hamiltonella and Regiella. These results indicate that Hamiltonella and Regiella had a negative effect on the longevity of sexual aphids of the A. pisum red morph clone,however,their ability to enhance host fitness by regulating the sex ratio and increasing the hatching rate of fertilised eggs. There might be a competitive effect between Hamiltonella and Regiella.

Key words: Acyrthosiphon pisum, Bacterial secondary symbionts, Sexual generation, Development, Reproduction

摘要： 为研究次生共生菌对红色型豌豆蚜有性世代生物学特性的影响，使用同时感染Hamiltonella和Regiella的红色型豌豆蚜作为初始蚜虫，首先运用抗生素喂食法得到未感染克隆，再制备3个人工感染克隆。将各克隆置于短日照环境下调查性比，再使用性蚜制作9个交配组合，统计各组合中性蚜交配后寿命、单雌产卵数和受精成功率，并调查受精卵的孵化历期和孵化率。结果发现：感染Hamiltonella或Regiella后，豌豆蚜性比会变得偏雌，且性蚜寿命也会减短；当性雌蚜感染时，其单雌产卵数显著减少，而雄蚜感染会导致受精成功率下降；虽然次生共生菌对受精卵孵化历期无影响，但是感染次生共生菌的受精卵孵化率显著上升；同时感染Hamiltonella和Regiella后并未发生相加效应。因此，尽管Hamiltonella和Regiella对豌豆蚜性蚜寿命有负面影响，但它们能通过调节性比、提高受精卵孵化率的方式来增强宿主适合度。Hamiltonella和Regiella之间可能存在竞争效应。

关键词: 豌豆蚜, 次生共生菌, 有性世代, 发育, 繁殖