Comprehensive Evaluation of Cadmium Tolerance and Enrichment Ability of Sweet Sorghum at Seedling Stage

doi:10.11733/j.issn.1007-0435.2023.10.016

Acta Agrestia Sinica ›› 2023, Vol. 31 ›› Issue (10): 3040-3049.DOI: 10.11733/j.issn.1007-0435.2023.10.016

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Comprehensive Evaluation of Cadmium Tolerance and Enrichment Ability of Sweet Sorghum at Seedling Stage

HUANG Juan, ZHOU Yu, ZHANG Ya-qin, LI Ze-bi, WANG Qiu-yue, XI Jiang, WU Xiu-lin

Institute of Characteristic Crops Research, Chongqing Academy of Agricultural Sciences, Chongqing 402160, China

Received:2023-03-27 Revised:2023-06-29 Online:2023-10-15 Published:2023-11-02

甜高粱苗期镉耐性和富集能力综合评价

黄娟, 周瑜, 张亚勤, 李泽碧, 王秋月, 奚江, 吴修林

重庆市农业科学院特色作物研究所, 重庆 402160

通讯作者: 周瑜,E-mail:xinganmermer@163.com
作者简介:黄娟(1989-),女,汉族,重庆巴南人,硕士,助理研究员,主要从事高粱遗传改良与栽培生理研究,E-mail:hj.198800@163.com
基金资助:
重庆市自然科学基金面上项目（cstc2019jcyj-msxmX0125）；重庆市科研机构绩效激励引导专项（cstc2022jxjl80016）；重庆市技术创新与应用发展专项重点项目（cstc2021jscx-gksbX0014）；重庆市财政项目（cqaas2023sjczqn027）资助

Abstract

Abstract: The objective of this study was to screen out and identify the varieties of sweet sorghum (Sorghum bicolor L.) with strong concentration of Cd (Cadmium),and to provide excellent materials for the remediation of Cd contaminated soil. A sand culture pot experiment with Cd stress (5 mg·kg^-1 Cd²⁺) and control (0 mg·kg^-1) treatments was conducted to investigate the growth characteristics,physiological indicators,and Cd content in roots and shoots of seedlings of 26 sweet sorghum varieties. The Cd tolerance and enrichment ability of 26 sweet sorghum varieties was evaluated by principal components analysis,membership function analysis and systematic cluster analysis. The results showed that there were significant differences in root length,plant height,root fresh weight,root dry weight,shoots fresh weight,shoots dry weight,chlorophyll content,POD activity,SOD activity and MDA content between varieties and Cd treatment (P<0.01). The 10 individual indicators could be classified into 3 independent comprehensive components by principal components analysis. The cadmium-tolerance comprehensive evaluation value (D value) of different cultivars at seedling stage was obtained by membership function analysis. Three varieties with strong Cd tolerance were selected by cluster analysis of D value. Twenty-six sweet sorghum varieties were divided into 4 categories by euclidean distance method based on the D value,bioconcentration factor coefficient and transfer coefficient. In conclusion ‘Liaotian 6’ and ‘F10’ were suitable for phytoremediation in Cd contaminated soil because of their higher tolerance to Cd and better enrichment ability.

Key words: Sweet sorghum, Cadmium tolerance, Cadmium enrichment ability, Comprehensive evaluation

摘要： 为筛选和鉴定出镉（Cadmium，Cd）富集能力强的甜高粱（Sorghum bicolor L.）品种，以期为利用甜高粱修复Cd污染土壤提供优异材料。本研究以26个甜高粱品种为材料，采用沙培盆栽试验，设置Cd胁迫（5 mg·kg^-1 Cd²⁺）和对照（0 mg·kg^-1）2个处理，测定苗期甜高粱生长特性和生理指标以及根部、茎叶的Cd含量，以各单项指标的耐性指数作为衡量耐Cd性的依据，运用主成分分析、隶属函数法和系统聚类综合评价Cd耐性和富集能力。结果表明：根长、株高、根鲜重、根干重、茎叶鲜重、茎叶干重、叶绿素含量、POD活性、SOD活性、MDA含量在品种和Cd处理间均存在极显著差异（P<0.01）。主成分分析将10个单项指标转化成3个相互独立的综合指标，利用隶属函数法分析，得到不同品种苗期耐Cd性综合评价值（D值）；通过对D值进行聚类分析，筛选出3份耐Cd性强的品种；进一步采用欧氏距离法对D值、富集系数和转移系数进行聚类分析，将26个甜高粱品种划分成4类，其中‘辽甜6号’和‘F10’属于Cd耐性强且富集转移性强的品种，适用于修复土壤Cd污染。

关键词: 甜高粱, Cd耐性, Cd富集能力, 综合评价

CLC Number:

S156

HUANG Juan, ZHOU Yu, ZHANG Ya-qin, LI Ze-bi, WANG Qiu-yue, XI Jiang, WU Xiu-lin. Comprehensive Evaluation of Cadmium Tolerance and Enrichment Ability of Sweet Sorghum at Seedling Stage[J]. Acta Agrestia Sinica, 2023, 31(10): 3040-3049.

黄娟, 周瑜, 张亚勤, 李泽碧, 王秋月, 奚江, 吴修林. 甜高粱苗期镉耐性和富集能力综合评价[J]. 草地学报, 2023, 31(10): 3040-3049.

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Comprehensive Evaluation of Cadmium Tolerance and Enrichment Ability of Sweet Sorghum at Seedling Stage

甜高粱苗期镉耐性和富集能力综合评价

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