Characterization and Analysis of SWEET Gene Family and Response to Abiotic Stress in Triticosecale

doi:10.11733/j.issn.1007-0435.2023.11.010

Acta Agrestia Sinica ›› 2023, Vol. 31 ›› Issue (11): 3310-3321.DOI: 10.11733/j.issn.1007-0435.2023.11.010

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Characterization and Analysis of SWEET Gene Family and Response to Abiotic Stress in Triticosecale

LI Gen, NIU Kui-ju

College of Pratacultureal Science/Gansu Agricultural University/Key Laboratory of Grassland Ecosystem of Education Ministry/Sino-U. S. Center for Grazingland Ecosystem Sustainability, Lanzhou, Gansu Province 730070, China

Received:2023-07-27 Revised:2023-08-31 Online:2023-11-15 Published:2023-12-01

小黑麦SWEET家族基因鉴定及其在不同逆境下表达模式分析

李根, 牛奎举

甘肃农业大学草业学院/草业生态系统教育部重点实验室/甘肃省草业工程实验室/中-美草地畜牧可持续发展研究中心, 甘肃兰州 730070

通讯作者: 牛奎举,E-mail:niukj@gsau.edu.cn
作者简介:李根(2002-),男,汉族,山东青岛人,本科生,主要从事植物分子生物学的研究,E-mail:15265276700@163.com;
基金资助:
省部共建干旱生境作物学国家重点实验室开放基金课题(GSCS-2021-12)；甘肃省自然科学基金重点研发项目(20YF8NA129)资助

Abstract

Abstract: The SWEET (sugars will finally be exported transporter) family is a new class of sugar transporters discovered in recent years. It plays an important role in maintaining plant growth and development,physiological metabolism,stress response in adversity,and plant-pathogen interaction. To date,a comprehensive analysis of SWEET members in triticale (× Triticosecale) has not been carried out. Therefore,based on the transcriptome database of triticale,16 TwSWEET genes were identified by homology search. Bioinformatics analysis showed that the TwSWEET gene family of triticale could be divided into four branches in an evolutionary relationship,which was highly conserved. They contain 6~7 transmembrane helices(TMH) with a total of 10 motifs. Subcellular localization results showed that most TwSWEETs were located on the plasma membrane. The expression patterns of SWEETs genes in the roots and leaves of triticale under drought (20% PEG) and cold (4℃) stress were further analyzed according to qPCR technology. The prediction of subcellular localization results showed that most of the TwSWEETs genes responded to drought and cold stresses,and the relative expression levels of the genes were significantly up-regulated or down-regulated,such as TwSWEET2a,TwSWEET6a,TwSWEET12, TwSWEET16. These genes could be used as candidate genes for further molecular functional studies on the responses of triticale to drought or cold stresses. Overall,the present study provides a reference for further study on the roles of TwSWEETs genes in triticale under abiotic stress.

Key words: SWEET sugar transporter, Bioinformatics analysis, Abiotic stress, Expression pattern

摘要： SWEET(Sugars will eventually be exported transporter)是近年来新发现的一类糖转运体,它在维持植物体生长发育、生理代谢、逆境胁迫响应、植物与病原菌互作等方面具有重要作用。截至目前，小黑麦(×Triticosecale)SWEET家族成员尚未被鉴定。因此，本研究基于小黑麦的转录组数据库，通过同源性检索共鉴定出16条TwSWEETs基因，生物信息学分析表明小黑麦TwSWEETs基因家族在进化关系上可分为四个枝，具有较强的保守性。它们含有6~7个跨膜螺旋结构(Transmembrane helices,TMH)，共有10个motif，亚细胞定位结果预测显示多数TwSWEETs定位于质膜上。进一步利用qPCR技术分析了TwSWEETs基因在干旱(20% PEG)、寒冷(4℃)胁迫下小黑麦根系和叶片的表达模式，结果表明绝大多数TwSWEET基因的相对表达量在受到胁迫后会显著上调或下调(如TwSWEET2a，TwSWEET6a，TwSWEET12，TwSWEET16)，可作为小黑麦响应干旱或寒冷胁迫的候选基因。本研究结果将为深入解析小黑麦TwSWEET基因的功能及其调控小黑麦干旱、寒冷胁迫的响应机制提供理论依据。

关键词: SWEET糖转运蛋白, 生物信息学分析, 逆境胁迫, 表达模式

CLC Number:

S512.1

LI Gen, NIU Kui-ju. Characterization and Analysis of SWEET Gene Family and Response to Abiotic Stress in Triticosecale[J]. Acta Agrestia Sinica, 2023, 31(11): 3310-3321.

李根, 牛奎举. 小黑麦SWEET家族基因鉴定及其在不同逆境下表达模式分析[J]. 草地学报, 2023, 31(11): 3310-3321.

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Characterization and Analysis of SWEET Gene Family and Response to Abiotic Stress in Triticosecale

小黑麦SWEET家族基因鉴定及其在不同逆境下表达模式分析

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