The Effects of Different Chemical Reagents on Germination Characteristics of Allium senescens L. Seed

doi:10.11733/j.issn.1007-0435.2024.10.018

Acta Agrestia Sinica ›› 2024, Vol. 32 ›› Issue (10): 3176-3184.DOI: 10.11733/j.issn.1007-0435.2024.10.018

The Effects of Different Chemical Reagents on Germination Characteristics of Allium senescens L. Seed

FU Chu-han, SONG Xue, LI Hao-tian, SUN Xue-tong, LI Jia-hong, HUA Yi, ZHU Wen-bo, HAN Zhi-hao, JIA Ming-han, QI Jing-jing, XIAO Zheng-qin, QIN Li-gang

College of Animal Science, Northeast Agricultural University, Harbin, Heilongjiang Province 150030, China

Received:2024-05-15 Revised:2024-06-19 Published:2024-11-04

不同化学试剂处理对山韭种子萌发特性的影响

付楚涵, 宋雪, 李浩天, 孙雪铜, 李家红, 华毅, 朱文博, 韩志豪, 贾茗涵, 戚晶晶, 肖正琴, 秦立刚

东北农业大学动物科学技术学院, 黑龙江哈尔滨 150030

通讯作者: 秦立刚,E-mail:qinligang@neau.edu.cn
作者简介:付楚涵(2001-)，女，汉族，黑龙江齐齐哈尔人，硕士研究生，主要从事草地植物资源利用研究，E-mail:fuchuhan01@163.com
基金资助:
黑龙江省优秀青年基金(YQ2023C013)资助

Abstract

Abstract: The objective of this research was to study the effects of Carbendazim (MBC),Hydrogen peroxide (H₂O₂) and Potassium permanganate (KMnO₄) on the germination of Allium senescens L. seeds. To conduct the experiments,A. senescens seeds were used and subjected to various concentrations of MBC (100,200,300,400,500-fold dilution),H₂O₂ (0.5%,1.0%,1.5%,2.0%,2.5%) and KMnO₄ (0.1%,0.2%,0.4%,0.8%,1.6%). The changes of germination indexes,physiological indexes and contents of endogenous hormones in different exogenous additives were analyzed. The results showed that compared with CK,200 times MBC,1.0%H₂O₂,0.4%KMnO₄ significantly promoted seed germination(P＜0.05),and significantly increased soluble sugar content,lipase enzymatic activity,neutral protease enzymatic activity and Ethrel (ETH) content (P<0.05). The results of principal component analysis and correlation analysis showed that the germination potential,percentage of germination,germination index and vital index of the seeds treated with MBC and KMnO₄ had a positive correlation with the enzyme activity. The germination energy,percentage of germination,germination index and vital index of the seeds treated with H₂O₂ were linked to soluble sugar and soluble protein. The comprehensive analysis showed that 200 times MBC and 1.0% H₂O₂increased the endogenous ETH content of A. senescens plumule to improve the germination ability of A. senescens seeds(P＜0.05),and 0.4% KMnO₄ not only increased the endogenous ETH content of A. senescens (P＜0.05),but also increased the endogenous Gibberellins (GA₃) content to promote the seed germination(P＜0.05).

Key words: Allium senescens L., Chemical reagents, Seed germination, Principal component analysis

摘要： 为探明多菌灵(Carbendazim，MBC)、过氧化氢(Hydrogen peroxide，H₂O₂)、高锰酸钾(Potassium permanganate，KMnO₄)对山韭(Allium senescens L.)种子萌发过程的影响，本研究以山韭(Allium Senescens L.)种子为试验材料，分析不同浓度MBC(100,200,300,400,500 倍)、H₂O₂(0.5%,1.0%,1.5%,2.0%,2.5%)、KMnO₄(0.1%,0.2%,0.4%,0.8%,1.6%)处理下种子萌发指标、生理指标及内源激素的变化规律。结果表明：与CK相比，200倍MBC、1.0% H₂O₂、0.4% KMnO₄对山韭种子的萌发有显著促进作用(P＜0.05)，可溶性糖含量、脂肪酶活性、中性蛋白酶活性、乙烯(Ethrel，ETH)含量均显著提高(P＜0.05)。主成分分析和相关性分析表明，MBC和KMnO₄处理的山韭种子发芽率、发芽势、发芽指数和活力指数与酶活性呈正相关关系；H₂O₂处理的山韭种子发芽率、发芽势、发芽指数和活力指数与可溶性糖、可溶性蛋白有正相关关系。综合分析表明，200倍MBC、1.0% H₂O₂主要通过显著提高山韭胚芽内源ETH含量(P＜0.05)，而0.4% KMnO₄通过显著提高内源ETH含量和内源赤霉素(Gibberellins，GA₃)含量(P＜0.05)来促进山韭种子萌发。

关键词: 山韭, 化学试剂, 种子萌发, 主成分分析

CLC Number:

S543.9

FU Chu-han, SONG Xue, LI Hao-tian, SUN Xue-tong, LI Jia-hong, HUA Yi, ZHU Wen-bo, HAN Zhi-hao, JIA Ming-han, QI Jing-jing, XIAO Zheng-qin, QIN Li-gang. The Effects of Different Chemical Reagents on Germination Characteristics of Allium senescens L. Seed[J]. Acta Agrestia Sinica, 2024, 32(10): 3176-3184.

付楚涵, 宋雪, 李浩天, 孙雪铜, 李家红, 华毅, 朱文博, 韩志豪, 贾茗涵, 戚晶晶, 肖正琴, 秦立刚. 不同化学试剂处理对山韭种子萌发特性的影响[J]. 草地学报, 2024, 32(10): 3176-3184.

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The Effects of Different Chemical Reagents on Germination Characteristics of Allium senescens L. Seed

不同化学试剂处理对山韭种子萌发特性的影响

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